Maize TIR-only Proteins ZmTIR1 and ZmTIR2, but not ZmTIR3 Confer Auto-active Hypersensitive Response Likely by Forming Condensation DOI Creative Commons
Zhen Kang, Qidong Ge,

M. Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

SUMMARY Nucleotide binding, leucine-rich-repeat (NLR) proteins are the major intracellular receptors for defending against pathogen infection. The recognition between NLRs and secreted effectors often triggers a localized programmed cell death termed hypersensitive response (HR). Despite significant progresses have been achieved in understanding canonical with N-terminal Toll/interleukin-1 receptor (TIR) domains, molecular mechanisms underlying TIR-only plant immune responses remain unclear. In this study, we identified six TIR-containing maize, including three proteins. Functional analysis showed that ZmTIR1 ZmTIR2, but not ZmTIR3, confer autoactive HR when transiently expressed N. benthamiana . autoactivity conferred by ZmTIR2 depends on EDS1-PAD4-RNL module their putative NADase activities. Interestingly, predominantly localize punctate dots likely form condensation, while ZmTIR3 mainly localizes cytoplasm nucleus. Two specific amino acids BB-loop region were to be required ZmTIR1- ZmTIR2-mediated condensation formation auto-HR. Furthermore, ZmTIR induced Cochliobolus heterostrophus , causal agent of southern leaf blight (SLB) knock-down expression or decreased resistance SLB maize. Our study reveals novel mechanism monocot maize responses.

Language: Английский

Activation and inhibition of helper NLRs in TIR signaling of higher plants DOI
Hainan Tian, Yuelin Zhang

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Maize TIR-only Proteins ZmTIR1 and ZmTIR2, but not ZmTIR3 Confer Auto-active Hypersensitive Response Likely by Forming Condensation DOI Creative Commons
Zhen Kang, Qidong Ge,

M. Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

SUMMARY Nucleotide binding, leucine-rich-repeat (NLR) proteins are the major intracellular receptors for defending against pathogen infection. The recognition between NLRs and secreted effectors often triggers a localized programmed cell death termed hypersensitive response (HR). Despite significant progresses have been achieved in understanding canonical with N-terminal Toll/interleukin-1 receptor (TIR) domains, molecular mechanisms underlying TIR-only plant immune responses remain unclear. In this study, we identified six TIR-containing maize, including three proteins. Functional analysis showed that ZmTIR1 ZmTIR2, but not ZmTIR3, confer autoactive HR when transiently expressed N. benthamiana . autoactivity conferred by ZmTIR2 depends on EDS1-PAD4-RNL module their putative NADase activities. Interestingly, predominantly localize punctate dots likely form condensation, while ZmTIR3 mainly localizes cytoplasm nucleus. Two specific amino acids BB-loop region were to be required ZmTIR1- ZmTIR2-mediated condensation formation auto-HR. Furthermore, ZmTIR induced Cochliobolus heterostrophus , causal agent of southern leaf blight (SLB) knock-down expression or decreased resistance SLB maize. Our study reveals novel mechanism monocot maize responses.

Language: Английский

Citations

0