Toward an integrated omics approach for plant biosynthetic pathway discovery in the age of AI

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Navigating the challenges of engineering composite specialized metabolite pathways in plants

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels target metabolites, alternative strategies such as pathway engineering heterologous systems like microbes (e.g., bacteria and fungi) or cell‐free can be employed. Another approach is plant engineering, which aims to either enhance the native production original reconstruct model system. Although increasing metabolite promising strategy, plants exotic pose significant challenges for genetic manipulation. Effective requires comprehensive prior knowledge genes enzymes involved, well precursor, intermediate, branching, final metabolites. Thus, thorough elucidation biosynthetic closely linked successful metabolic host systems. In this review, we highlight recent advances engineering. We focus on efforts engineer complex, multi‐step pathways that require expression at least eight transient three stable transformation. Reports complex stably transformed remain relatively scarce. discuss major hurdles overcoming them, followed by an overview achievements, challenges, solutions reconstitution through Recent including computer‐based predictions offer platforms sustainable plants.

Language: Английский

Synthetic Biology in Natural Product Biosynthesis

Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Synthetic biology has played an important role in the renaissance of natural products research during post-genomics era. The development and integration new tools have transformed workflow product discovery engineering, generating multidisciplinary interest field. In this review, we summarize recent developments biosynthesis from three different aspects. First, advances bioinformatics, experimental, analytical to identify associated with predicted biosynthetic gene clusters (BGCs) will be covered. This followed by extensive review on heterologous expression bacterial, fungal plant organisms. native host-independent paradigm identification, pathway characterization, enzyme is where synthetic most prominent role. Lastly, strategies engineer pathways for structural diversification complexity generation discussed, including assembly-line megasynthase precursor-directed modification, combinatorial biosynthesis.

Language: Английский