Energetic and genomic potential for hydrogenotrophic, formatotrophic, and acetoclastic methanogenesis in surface-expressed serpentinized fluids of the Samail Ophiolite DOI Creative Commons
Alta Howells, Laura Quinn,

Marília gabryela vieira da silva

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 31, 2025

Serpentinization, the reaction of water with ultramafic rock, produces reduced, hyperalkaline, and H2-rich fluids that support a variety hydrogenotrophic microbial metabolisms. Previous work indicates occurrence methanogenesis in from actively serpentinizing Samail Ophiolite Sultanate Oman. While those contain abundant H2 to fuel (CO2 + 4H2 ➔ CH4 2H2O), concentration CO2 is very low due hyperalkalinity (> pH 11) geochemistry fluids. As result, species such as formate acetate may be important alternative methanogenic substrates. In this study we quantified impact inorganic carbon, availability for metabolisms, across range fluid chemistries, terms (1) potential diffusive flux substrates cell, (2) Affinity (Gibbs energy change) associated metabolism, (3) "inventory" per kg fluid. parallel, assessed genomic conduct three modes same set consider results within quantitative framework availability. We find formatotrophic affords higher (greater energetic yield) than acetoclastic pristine serpentinized and, agreement previous studies, evidence methanogen genus Methanobacterium carry out methanogenesis, possibility even using bicarbonate supply CO2. Acetoclastic also shown energetically favorable these fluids, report first detection family Methanosarcinaceae, which forms distinct clade genome seafloor hydrothermal vent field, Lost City. These demonstrate applicability an interpreting ecology systems.

Language: Английский

Energetic and genomic potential for hydrogenotrophic, formatotrophic, and acetoclastic methanogenesis in surface-expressed serpentinized fluids of the Samail Ophiolite DOI Creative Commons
Alta Howells, Laura Quinn,

Marília gabryela vieira da silva

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 31, 2025

Serpentinization, the reaction of water with ultramafic rock, produces reduced, hyperalkaline, and H2-rich fluids that support a variety hydrogenotrophic microbial metabolisms. Previous work indicates occurrence methanogenesis in from actively serpentinizing Samail Ophiolite Sultanate Oman. While those contain abundant H2 to fuel (CO2 + 4H2 ➔ CH4 2H2O), concentration CO2 is very low due hyperalkalinity (> pH 11) geochemistry fluids. As result, species such as formate acetate may be important alternative methanogenic substrates. In this study we quantified impact inorganic carbon, availability for metabolisms, across range fluid chemistries, terms (1) potential diffusive flux substrates cell, (2) Affinity (Gibbs energy change) associated metabolism, (3) "inventory" per kg fluid. parallel, assessed genomic conduct three modes same set consider results within quantitative framework availability. We find formatotrophic affords higher (greater energetic yield) than acetoclastic pristine serpentinized and, agreement previous studies, evidence methanogen genus Methanobacterium carry out methanogenesis, possibility even using bicarbonate supply CO2. Acetoclastic also shown energetically favorable these fluids, report first detection family Methanosarcinaceae, which forms distinct clade genome seafloor hydrothermal vent field, Lost City. These demonstrate applicability an interpreting ecology systems.

Language: Английский

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