Avian photoreceptor homologies and the origin of double cones DOI Creative Commons
Yu Liu, Emily G. Hurley,

Yohey Ogawa

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Birds possess the most complex photoreceptor system among vertebrates, with one rod and six cone types, including four single cones (violet, blue, green, red) two constituent cells of double (DC-P DC-A). The evolutionary relationships avian photoreceptors to those other vertebrate taxa have not been systematically explored. Here, we perform single-cell RNA sequencing (scRNA-seq) on retinas newly hatched chickens trace cell-type homologies across species. Analysis differentially expressed transcription factors (TFs) suggests that rods types correspond cognate cell in fish placental mammals, whereas a distinct origin. We propose DC-P arose from an ancestral red cone, as revealed by expression fate determinants thyroid hormone receptor β (THRB) SAMD7, DC-A may arisen blue suggested TFs FOXQ2 SKOR1. These signatures are shared green anole lizard (Anolis carolinensis), suggesting conservation throughout Sauropsida. Consistent our hypothesis, CRISPR-mediated knockout THRB causes loss DC-P, but DC-A, appearance supernumerary cones, suggestive direct transfating. Furthermore, cis-regulatory analysis separate enhancers control opsin consistent origins. Taken together, studies suggest origins respectively.

Language: Английский

Avian photoreceptor homologies and the origin of double cones DOI Creative Commons
Yu Liu, Emily G. Hurley,

Yohey Ogawa

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Birds possess the most complex photoreceptor system among vertebrates, with one rod and six cone types, including four single cones (violet, blue, green, red) two constituent cells of double (DC-P DC-A). The evolutionary relationships avian photoreceptors to those other vertebrate taxa have not been systematically explored. Here, we perform single-cell RNA sequencing (scRNA-seq) on retinas newly hatched chickens trace cell-type homologies across species. Analysis differentially expressed transcription factors (TFs) suggests that rods types correspond cognate cell in fish placental mammals, whereas a distinct origin. We propose DC-P arose from an ancestral red cone, as revealed by expression fate determinants thyroid hormone receptor β (THRB) SAMD7, DC-A may arisen blue suggested TFs FOXQ2 SKOR1. These signatures are shared green anole lizard (Anolis carolinensis), suggesting conservation throughout Sauropsida. Consistent our hypothesis, CRISPR-mediated knockout THRB causes loss DC-P, but DC-A, appearance supernumerary cones, suggestive direct transfating. Furthermore, cis-regulatory analysis separate enhancers control opsin consistent origins. Taken together, studies suggest origins respectively.

Language: Английский

Citations

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