Temporal evolution of insecticide resistance and bionomics in Anopheles funestus, a key malaria vector in Uganda DOI Creative Commons
Ambrose Oruni, Magellan Tchouakui, Carlos S. Djoko Tagne

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 30, 2024

Insecticide resistance escalation is decreasing the efficacy of vector control tools. Monitoring paramount in order to understand its evolution and devise effective counter-solutions. In this study, we monitored insecticide patterns, population bionomics genetic variants associated with over 3 years from 2021 2023 Uganda. Anopheles funestus s.s was predominant species Mayuge but evidence hybridization other An. group. Sporozoite infection rates were relatively very high a peak 20.41% March 2022. Intense pyrethroid seen against pyrethroids up 10-times diagnostic concentration partial recovery susceptibility PBO synergistic assays. Among bednets, only PBO-based nets (PermaNet 3.0 Top Olyset Plus) chlorfenapyr-based net (Interceptor G2) had mortality rates. Mosquitoes fully susceptible chlorfenapyr organophosphates, moderately resistant clothianidin carbamates. The allele frequency key P450, CYP9K1, marker constantly that for CYP6P9A/b low. Interestingly, report first detection alleles Ace1 gene (RS = ~ 13%) Rdl 21%, RR 4%) qRT-PCR revealed Cytochrome P450s CYP6P9A, CYP6P9b, CYP6P5 CYP6M7 consistently upregulated while glutathione-S-transferase (GSTE2) showed low expression. Our study shows complexity patterns underlying mechanisms, hence constant consistent spatial temporal monitoring crucial rapidly detect changing profiles which informing deployment counter interventions.

Language: Английский

Temporal evolution of insecticide resistance and bionomics in Anopheles funestus, a key malaria vector in Uganda DOI Creative Commons
Ambrose Oruni, Magellan Tchouakui, Carlos S. Djoko Tagne

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 30, 2024

Insecticide resistance escalation is decreasing the efficacy of vector control tools. Monitoring paramount in order to understand its evolution and devise effective counter-solutions. In this study, we monitored insecticide patterns, population bionomics genetic variants associated with over 3 years from 2021 2023 Uganda. Anopheles funestus s.s was predominant species Mayuge but evidence hybridization other An. group. Sporozoite infection rates were relatively very high a peak 20.41% March 2022. Intense pyrethroid seen against pyrethroids up 10-times diagnostic concentration partial recovery susceptibility PBO synergistic assays. Among bednets, only PBO-based nets (PermaNet 3.0 Top Olyset Plus) chlorfenapyr-based net (Interceptor G2) had mortality rates. Mosquitoes fully susceptible chlorfenapyr organophosphates, moderately resistant clothianidin carbamates. The allele frequency key P450, CYP9K1, marker constantly that for CYP6P9A/b low. Interestingly, report first detection alleles Ace1 gene (RS = ~ 13%) Rdl 21%, RR 4%) qRT-PCR revealed Cytochrome P450s CYP6P9A, CYP6P9b, CYP6P5 CYP6M7 consistently upregulated while glutathione-S-transferase (GSTE2) showed low expression. Our study shows complexity patterns underlying mechanisms, hence constant consistent spatial temporal monitoring crucial rapidly detect changing profiles which informing deployment counter interventions.

Language: Английский

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