Differentially expressed and alternately spliced genes as a novel tool for genotoxicity: a computerized study in ATT-myc transgenic mice for the recognition of genotoxic and non-genotoxic chemical DOI Creative Commons
Mansour A. Alghamdi,

Eman Mohamad El Nashar,

Mahmoud Elalfy

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 28, 2025

Transgenic mice and gene expression in analyses were employed to evaluate hazardous chemicals. Mice received weekly doses of NDEA (75 mg/kg) for six weeks twice-weekly BHT (300 eight weeks. Gene splicing alterations the livers transgenic each treatment examined using MouseExon10ST array. Six hybridizations revealed 645 genes with significant changes, 181 showed both (p < 0.01). Furthermore, 2021 demonstrated exon-group interactions, indicating potential alternative splicing. Pathway analysis identified enriched groups GOMolFn, GOProcess, GOCellLoc, classes, a higher representation alternatively spliced expressed Among top was TAT, which encodes mitochondrial enzyme tyrosine aminotransferase, involved metabolism recognized as novel tumor suppressor linked hepatocellular carcinoma (HCC). Additionally, HNF-4, transcription factor, plays crucial role TAT expression. This method can be used identify genotoxic compounds att-myc model short-term toxicity.

Language: Английский

Differentially expressed and alternately spliced genes as a novel tool for genotoxicity: a computerized study in ATT-myc transgenic mice for the recognition of genotoxic and non-genotoxic chemical DOI Creative Commons
Mansour A. Alghamdi,

Eman Mohamad El Nashar,

Mahmoud Elalfy

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 28, 2025

Transgenic mice and gene expression in analyses were employed to evaluate hazardous chemicals. Mice received weekly doses of NDEA (75 mg/kg) for six weeks twice-weekly BHT (300 eight weeks. Gene splicing alterations the livers transgenic each treatment examined using MouseExon10ST array. Six hybridizations revealed 645 genes with significant changes, 181 showed both (p < 0.01). Furthermore, 2021 demonstrated exon-group interactions, indicating potential alternative splicing. Pathway analysis identified enriched groups GOMolFn, GOProcess, GOCellLoc, classes, a higher representation alternatively spliced expressed Among top was TAT, which encodes mitochondrial enzyme tyrosine aminotransferase, involved metabolism recognized as novel tumor suppressor linked hepatocellular carcinoma (HCC). Additionally, HNF-4, transcription factor, plays crucial role TAT expression. This method can be used identify genotoxic compounds att-myc model short-term toxicity.

Language: Английский

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