Strong Effects of Increased Hydrostatic Pressure on Polysaccharide‐Hydrolyzing Enzyme Activities in Coastal Seawater and Sediments DOI Creative Commons
C. Chad Lloyd,

J.P. Balmonte,

Ronnie N. Glud

et al.

Journal of Geophysical Research Biogeosciences, Journal Year: 2025, Volume and Issue: 130(2)

Published: Feb. 1, 2025

Abstract Heterotrophic microorganisms are responsible for transforming and respiring a substantial fraction of the organic matter produced by phytoplankton in surface ocean. Much this is composed polysaccharides, high‐molecular weight (HMW) sugars. To initiate degradation must produce extracellular enzymes right structural specificity to hydrolyze these complex structures. date, most measurements enzyme activities made at situ temperatures, but atmospheric pressure. However, previous studies have shown that hydrostatic pressure can impact functionality enzymes. Since deep sea communities may be seeded microbes from shallow waters, we aimed determine if affects performance coastal waters. extent which enzymatic microbial affected pressure, quantified seven polysaccharides under pressures ranging 0.1 MPa (atmospheric) 40 (equivalent 4,000 m). Enzyme pelagic were inhibited with increased while benthic more resistant Addition HMW resulted freely‐dissolved (<0.2 μm) strongly suggesting pressure‐resistant cell‐surface attached. Because inhibition varied polysaccharide, surmise complexity polysaccharide—and therefore number distinct required hydrolysis—is likely closely associated inhibition.

Language: Английский

Strong Effects of Increased Hydrostatic Pressure on Polysaccharide‐Hydrolyzing Enzyme Activities in Coastal Seawater and Sediments DOI Creative Commons
C. Chad Lloyd,

J.P. Balmonte,

Ronnie N. Glud

et al.

Journal of Geophysical Research Biogeosciences, Journal Year: 2025, Volume and Issue: 130(2)

Published: Feb. 1, 2025

Abstract Heterotrophic microorganisms are responsible for transforming and respiring a substantial fraction of the organic matter produced by phytoplankton in surface ocean. Much this is composed polysaccharides, high‐molecular weight (HMW) sugars. To initiate degradation must produce extracellular enzymes right structural specificity to hydrolyze these complex structures. date, most measurements enzyme activities made at situ temperatures, but atmospheric pressure. However, previous studies have shown that hydrostatic pressure can impact functionality enzymes. Since deep sea communities may be seeded microbes from shallow waters, we aimed determine if affects performance coastal waters. extent which enzymatic microbial affected pressure, quantified seven polysaccharides under pressures ranging 0.1 MPa (atmospheric) 40 (equivalent 4,000 m). Enzyme pelagic were inhibited with increased while benthic more resistant Addition HMW resulted freely‐dissolved (<0.2 μm) strongly suggesting pressure‐resistant cell‐surface attached. Because inhibition varied polysaccharide, surmise complexity polysaccharide—and therefore number distinct required hydrolysis—is likely closely associated inhibition.

Language: Английский

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