A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types DOI Creative Commons
Yoav Ben‐Simon, Marcus Hooper, Sujatha Narayan

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We use transcriptomic epigenomic cortical cell-type taxonomies from mouse human define marker genes putative enhancers create a large toolkit transgenic lines enhancer adeno-associated viruses (AAVs) selective targeting populations. report creation evaluation fifteen driver lines, two reporter >1,000 different AAV vectors covering most subclasses cells. tools reported here have been made publicly available, along with scaled process tool creation, evaluation, modification, they will enable diverse experimental strategies toward brain function.

Language: Английский

Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits DOI Creative Commons
Avery C. Hunker, Morgan Wirthlin,

Gursajan Gill

et al.

Neuron, Journal Year: 2025, Volume and Issue: 113(10), P. 1507 - 1524.e17

Published: May 1, 2025

We present an enhancer-AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct- indirect-pathway MSNs, Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable cell-type-specific labeling under experimental contexts. demonstrate direct pathway circuit-selective optogenetic perturbation behavior multiplex interneuron targeted analysis cellular features. Lastly, show conserved in vivo activity exemplary MSN enhancers rats macaques. This collection enhancer AAVs offers greater versatility compared available transgenic lines can readily be applied type circuit studies mammalian species beyond the mouse model.

Language: Английский

Citations

0
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types DOI Creative Commons
Yoav Ben‐Simon, Marcus Hooper, Sujatha Narayan

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We use transcriptomic epigenomic cortical cell-type taxonomies from mouse human define marker genes putative enhancers create a large toolkit transgenic lines enhancer adeno-associated viruses (AAVs) selective targeting populations. report creation evaluation fifteen driver lines, two reporter >1,000 different AAV vectors covering most subclasses cells. tools reported here have been made publicly available, along with scaled process tool creation, evaluation, modification, they will enable diverse experimental strategies toward brain function.

Language: Английский

Citations

0