Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Cross-linking and covalent labeling are common tools for protein structural elucidation by mass spectrometry. However, despite the importance of electrostatic interactions in proteins, there not many probing both acids amines. Therefore, we introduce novel solution-phase amine-to-acid cross-linkers single reagent amine acid labels utilizing carbodiimide chemistry. sites were determined using top-down enzymatic digestion approaches. Ubiquitin was chosen as a model isotopically labeled 13C-15N-glycine cross-linking/covalent glycyl-l-proline with spectrometry proof concept. Alpha synuclein, an intrinsically disordered that plays role multiple neurological disorders, also cross-linked/covalent these methods/reagents. We expect characterization techniques will play gaining insight into functionally pathologically important tendencies proteins.
Language: Английский