Neuroscience, Journal Year: 2023, Volume and Issue: 525, P. 47 - 50
Published: July 6, 2023
Language: Английский
Molecular and Cellular Endocrinology, Journal Year: 2020, Volume and Issue: 518, P. 110986 - 110986
Published: Aug. 21, 2020
Language: Английский
Citations
78
Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)
Published: Sept. 23, 2021
Abstract The four described species of Danionella are tiny, transparent fishes that mature at sizes between 10–15 mm, and represent some the most extreme cases vertebrate progenesis known to date. miniature adult size larval appearance , combined with a diverse behavioral repertoire linked sound production by males, have established as an important model for neurophysiological studies. external similarity different has offered challenge taxonomic identification using traditional characters, leading confusion over identity species. Using morphological molecular approaches, we show here extensively studied is not D. translucida but represents undescribed species, cerebrum n. sp. externally almost identical differs trenchantly in several internal characters. Molecular analyses confirm distinctiveness suggest two even sister taxa. Analysis evolution sexual dimorphisms associated Weberian apparatus reveals significant increases complexity from simpler condition found dracula complex conditions mirifica .
Language: Английский
Citations
77
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(10)
Published: Feb. 26, 2024
Motion is the basis of nearly all animal behavior. Evolution has led to some extraordinary specializations propulsion mechanisms among invertebrates, including mandibles dracula ant and claw pistol shrimp. In contrast, vertebrate skeletal movement considered be limited by speed muscle, saturating around 250 Hz. Here, we describe unique mechanism which
Language: Английский
Citations
9
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: Dec. 4, 2023
Due to the size and opacity of vertebrate brains, it has until now been impossible simultaneously record neuronal activity at cellular resolution across entire adult brain. As a result, scientists are forced choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging coarse-grained resolution. Bridging gap these spatial scales understanding remains major challenge in neuroscience. Here, we introduce blazed oblique plane perform brain-wide recording an vertebrate. Contrary common belief, find that inferences population near-independent scale: set randomly sampled neurons comparable predictive power as same number macrovoxels. Our work thus links with scope, challenges prevailing view macroscale methods generally inferior microscale techniques underscores value multiscale approaches studying activity.
Language: Английский
Citations
17
Nature, Journal Year: 2024, Volume and Issue: 631(8019), P. 118 - 124
Published: June 19, 2024
Abstract Locating sound sources such as prey or predators is critical for survival in many vertebrates. Terrestrial vertebrates locate by measuring the time delay and intensity difference of pressure at each ear 1–5 . Underwater, however, physics makes interaural cues very small, suggesting that directional hearing fish should be nearly impossible 6 Yet, has been confirmed behaviourally, although mechanisms have remained unknown decades. Several hypotheses proposed to explain this remarkable ability, including possibility evolved an extreme sensitivity minute differences might compare with particle motion signals 7,8 However, experimental challenges long hindered a definitive explanation. Here we empirically test these models transparent teleost Danionella cerebrum , one smallest 9,10 By selectively controlling motion, dissect sensory algorithm underlying acoustic startles. We find both are indispensable behaviour their relative phase controls its direction. Using micro-computed tomography optical vibrometry, further show D. structures implement mechanism. shares more than 15% living vertebrate species, widespread mechanism inferring
Language: Английский
Citations
6
Scientific Data, Journal Year: 2019, Volume and Issue: 6(1)
Published: Aug. 26, 2019
Abstract Studying neuronal circuits at cellular resolution is very challenging in vertebrates due to the size and optical turbidity of their brains. Danionella translucida , a close relative zebrafish, was recently introduced as model organism for investigating neural network interactions adult individuals. remains transparent throughout its life, has smallest known vertebrate brain possesses rich repertoire complex behaviours. Here we sequenced, assembled annotated genome employing hybrid Illumina/Nanopore read library well RNA-seq embryonic, larval mRNA. We achieved high assembly continuity using low-coverage long-read data large fraction transcriptome. This dataset will pave way molecular research targeted genetic manipulation this novel organism.
Language: Английский
Citations
30
Disease Models & Mechanisms, Journal Year: 2022, Volume and Issue: 15(12)
Published: Nov. 18, 2022
Danionella cerebrum is a new vertebrate model that offers an exciting opportunity to visualize dynamic biological processes in intact adult animals. Key advantages of this include its small size, life-long optical transparency, genetic amenability and short generation time. Establishing reliable method for longitudinal vivo imaging D. while maintaining viability will allow in-depth image-based studies various involved development, disease onset progression, wound healing, aging live animal. Here, both prolonged confocal using custom-designed 3D-printed chambers described. Two transgenic lines were created test the system, i.e. Tg(mpeg1:dendra2) Tg(kdrl:mCherry-caax). The first line was used macrophages microglia, second spatial registration. By approach, differences immune cell morphology behavior during homeostasis as well response stab or two-photon-induced brain injury observed fish over course several days.
Language: Английский
Citations
15
Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 43(45), P. 7456 - 7462
Published: Nov. 8, 2023
Environmentally appropriate social behavior is critical for survival across the lifespan. To support this flexible behavior, brain must rapidly perform numerous computations taking into account sensation, memory, motor-control, and many other systems. Further complicating process, individuals distinct behaviors adapted to unique demands of each developmental stage; indeed, newborn would not be in adulthood vice versa. However, our understanding neural circuit transitions supporting these behavioral has been limited. Recent advances dissection tools, as well adaptation tools use at early time points, helped uncover several novel mechanisms developmentally behavior. This review, associated Minisymposium, bring together neuroscience research model organisms ages. Together, work highlights regulated functional roles sensory cortex, prefrontal amygdala, habenula, thalamus interaction from infancy adulthood. These studies underscore need synthesis varied ages advance
Language: Английский
Citations
8
Developmental Dynamics, Journal Year: 2019, Volume and Issue: 248(11), P. 1144 - 1154
Published: June 22, 2019
Differences in postembryonic developmental trajectories can profoundly alter adult phenotypes and life histories. Thyroid hormone (TH) regulates metamorphosis many vertebrate taxa with multiphasic ecologies, alterations to TH metabolism underlie notable cases of paedomorphosis amphibians. We tested the requirement for multiple processes zebrafish, which has a monophasic ecology, asked if production was compromised paedomorphic Danionella.
Language: Английский
Citations
23
Developmental Dynamics, Journal Year: 2020, Volume and Issue: 250(4), P. 601 - 611
Published: Dec. 16, 2020
Abstract Background Species of Danionella rank among the smallest all vertebrates and their miniature size is correlated with an extreme case progenesis, resulting in tiny, transparent sexually mature individuals. Progenesis has affected entire skeleton , which 60 skeletal elements are absent, including some skull roofing bones. This lack a roof combined presence fully formed hearing sound producing apparatus led to being used as important model for neurophysiological studies. Results Using both based PGi analyses we investigate sequence heterochrony development dracula close relatives. Extreme heterochronic shifts affect appearance bony . includes delay most chondral bones, reduction or loss dermal bones that would otherwise form roof. In contrast, formation anterior region vertebral column, functionally Weberian apparatus, greatly accelerated. Conclusions Here show anatomical conditions favor brain research result have acted differentially across skeleton.
Language: Английский
Citations
21