The Endoplasmic Reticulum Unfolded Protein Response in Neurodegenerative Disorders and Its Potential Therapeutic Significance

Frontiers in Molecular Neuroscience, Journal Year: 2017, Volume and Issue: 10

Published: June 15, 2017

In eukaryotic cells, the endoplasmic reticulum (ER) is cell compartment involved in secretory protein translocation and quality control of folding. Different conditions can alter ER function, resulting accumulation unfolded or misfolded proteins within lumen. Such a condition, known as stress, elicits an integrated adaptive response (UPR) that aims to restore proteostasis pathway. Conversely, prolonged stress insufficient response, UPR signalling causes death. dysfunctions are contribute neuronal degeneration several human diseases, including Alzheimer, Parkinson Huntington disease amyotrophic lateral sclerosis. The correlations between its signal transduction pathway with neuropathological changes well established. addition, much evidence suggests genetic pharmacological modulation could represent effective strategy for minimising progressive loss neurodegenerative diseases. Here, we review recent results describing main cellular mechanisms linking neurodegeneration. Furthermore, provide up-to-date panoramic view currently pursued strategies ameliorating toxic effects unfolding by targeting

Language: Английский

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Regulation of neurogenesis by calcium signaling

Cell Calcium, Journal Year: 2016, Volume and Issue: 59(2-3), P. 124 - 134

Published: March 1, 2016

Language: Английский

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A Time Course Analysis of the Electrophysiological Properties of Neurons Differentiated from Human Induced Pluripotent Stem Cells (iPSCs)

PLoS ONE, Journal Year: 2014, Volume and Issue: 9(7), P. e103418 - e103418

Published: July 29, 2014

Many protocols have been designed to differentiate human embryonic stem cells (ESCs) and induced pluripotent (iPSCs) into neurons. Despite the relevance of electrophysiological properties for proper neuronal function, little is known about evolution over time important parameters in iPSC-derived Yet, understanding development basic characteristics neurons critical evaluating their usefulness translational research. Therefore, we analyzed forebrain differentiated from iPSCs, day 31 55 after initiation differentiation. We assayed developmental progression various properties, including resting membrane potential, action sodium potassium channel currents, somatic calcium transients synaptic activity. During maturation neurons, potential became more negative, expression voltage-gated channels increased, capable generating potentials following adequate depolarization and, at 48-55, 50% were firing response a prolonged depolarizing current step, which 30% produced multiple potentials. The percentage exhibiting miniature excitatory post-synaptic currents increased with significant increase frequency amplitude. These changes associated an Ca2+ transient frequency. Co-culturing mouse glial enhanced as compared pure cultures. This study demonstrates importance properly status newly generated when using cell technology, mature time.

Language: Английский

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Induction of Calcium Influx in Cortical Neural Networks by Nanomagnetic Forces

ACS Nano, Journal Year: 2016, Volume and Issue: 10(2), P. 2331 - 2341

Published: Jan. 25, 2016

Nanomagnetic force stimulation with ferromagnetic nanoparticles was found to trigger calcium influx in cortical neural networks without observable cytotoxicity. Stimulated showed an average of 20% increment fluorescence signals and a heightened frequency spiking. These effects were also confined spatially areas engineered high magnetic field gradients. Furthermore, blockage N-type channels inhibited the stimulatory nanomagnetic forces, suggesting role mechano-sensitive ion mediating influx.

Language: Английский

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Triboelectric Nanogenerator Accelerates Highly Efficient Nonviral Direct Conversion and In Vivo Reprogramming of Fibroblasts to Functional Neuronal Cells

Advanced Materials, Journal Year: 2016, Volume and Issue: 28(34), P. 7365 - 7374

Published: June 15, 2016

Triboelectric nanogenerators (TENGs) can be an effective cell reprogramming platform for producing functional neuronal cells therapeutic applications. stimulation accelerates nonviral direct conversion of induced from fibroblasts, increases the efficiency, and induces highly matured phenotypes with improved electrophysiological functionalities. TENG devices may also used biomedical in vivo reprogramming. As a service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed re-organized online delivery, but not copy-edited or typeset. Technical support issues arising (other than missing files) should addressed Please note: The publisher is responsible content functionality any Any queries content) directed corresponding author article.

Language: Английский

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Human neuronal maturation comes of age: cellular mechanisms and species differences

Nature reviews. Neuroscience, Journal Year: 2023, Volume and Issue: 25(1), P. 7 - 29

Published: Nov. 23, 2023

Language: Английский

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Information Processes

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 197 - 325

Published: Jan. 1, 2024

Language: Английский

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Defects DuringMecp2Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms

Cerebral Cortex, Journal Year: 2015, Volume and Issue: 26(6), P. 2517 - 2529

Published: May 15, 2015

MeCP2 is associated with several neurological disorders; of which, Rett syndrome undoubtedly represents the most frequent. Its molecular roles, however, are still unclear, and data from animal models often describe adult, symptomatic stages, while functions during embryonic development remain elusive. We pattern timing Mecp2 expression in neocortex highlighting its low but consistent virtually all cells show unexpected occurrence transcriptional defects null samples at a stage largely preceding onset overt symptoms. Through deregulated ionic channels glutamatergic receptors, lack early neuronal maturation leads to reduction responsiveness stimuli. suggest that such features concur morphological alterations begin affecting neurons around perinatal age become evident later adulthood. indicate as key modulator mechanisms regulating cerebral cortex development. Neurological phenotypes MECP2 patients could thus be cumulative result different adverse events already present stages when no obvious signs pathology worsened by impairments central nervous system maintenance functionality.

Language: Английский

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Genetic and biochemical changes of the serotonergic system in migraine pathobiology

The Journal of Headache and Pain, Journal Year: 2017, Volume and Issue: 18(1)

Published: Feb. 13, 2017

Migraine is a brain disorder characterized by piercing headache which affects one side of the head, located mainly at temples and in area around eye. imparts substantial suffering to family addition sufferer, particularly as it three times more women than men most prevalent between ages 25 45, years child rearing. typically occurs individuals with genetic predisposition aggravated specific environmental triggers. Attempts study biochemistry migraine began early 1960s were primarily directed serotonin metabolism after an increase 5-hydroxyindoleacetic acid (5-HIAA), main metabolite was observed urine migraineurs. Genetic biochemical studies have focused on neurotransmitter serotonin, considering receptor binding, transport synthesis investigated serotonergic mediators including enzymes, receptors well intermediary metabolites. These been assayed blood, CSF accessible fluids. More recently PET imaging technology integrated metabolomics systems biology platform are being applied biology. The general trend that patients alterations detected biological fluids different from controls, however interpretation significance these peripheral changes unresolved. In this review we present system metabolic routes for discuss results regard brain, cerebrospinal fluid, saliva, platelets, plasma patients.

Language: Английский

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Chitosan-based hydrogel implants enriched with calcium ions intended for peripheral nervous tissue regeneration

Carbohydrate Polymers, Journal Year: 2015, Volume and Issue: 136, P. 764 - 771

Published: Oct. 5, 2015

Language: Английский

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