Biomolecules,
Journal Year:
2024,
Volume and Issue:
15(1), P. 38 - 38
Published: Dec. 31, 2024
Nicotinamide
adenine
dinucleotide
(NAD)
is
a
critical
cofactor
in
mitochondrial
energy
production.
The
NADH/NAD+
ratio,
reflecting
the
balance
between
NADH
(reduced)
and
NAD+
(oxidized),
key
marker
for
severity
of
diseases.
We
recently
developed
streamlined
LC-MS/MS
method
precise
measurement
NAD+.
Utilizing
this
technique,
we
quantified
levels
fibroblasts
derived
from
pediatric
patients
Leigh
syndrome
mouse
model
which
respiratory
chain
complex
I
subunit
Ndufs4
knocked
out
(KO).
In
patient-derived
fibroblasts,
did
not
differ
significantly
those
healthy
controls
(p
=
0.79);
however,
were
elevated
0.04),
indicating
increased
reductive
stress.
This
increase,
observed
despite
comparable
total
NAD(H)
groups,
was
attributed
to
levels.
Similarly,
model,
KO
group
0.002),
further
suggesting
that
elevation
drives
expected
outperform
conventional
assays,
such
as
lactate,
providing
simpler
more
reliable
means
assessing
disease
progression.
Reproduction,
Journal Year:
2024,
Volume and Issue:
167(6)
Published: March 12, 2024
In
light
of
the
increasing
age
first-time
fathers,
this
article
summarizes
current
scientific
knowledge
base
on
reproductive
aging
in
male,
including
sperm
quality
and
health
impacts
for
offspring.
The
emerging
role
NAD
decline
is
highlighted.
Aging,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 2, 2025
Werner
syndrome
(WS),
caused
by
mutations
in
the
RecQ
helicase
WERNER
(WRN)
gene,
is
a
classical
accelerated
aging
disease
with
patients
suffering
from
several
metabolic
dysfunctions
without
cure.
While,
as
we
previously
reported,
depleted
NAD+
causes
accumulation
of
damaged
mitochondria,
leading
to
compromised
metabolism,
how
mitochondrial
changes
WS
and
impact
on
pathologies
were
unknown.
We
show
that
loss
WRN
increases
senescence
mesenchymal
stem
cells
(MSCs)
likely
related
dysregulation
pathways.
In
line
this,
augmentation,
via
supplementation
nicotinamide
riboside,
reduces
improves
profiles
MSCs
knockout
(WRN-/-)
primary
fibroblasts
derived
compared
controls.
Moreover,
deficiency
results
decreased
(measured
indirectly
mitochondrially-expressed
PARP
activity),
altered
expression
key
salvage
pathway
enzymes,
including
NMNAT1
NAMPT;
ChIP-seq
data
analysis
unveils
potential
co-regulatory
axis
between
NMNATs,
important
for
chromatin
stability
DNA
metabolism.
However,
restoration
or
cellular
not
sufficient
reinstall
proliferation
immortalized
siRNA-mediated
knockdown
WRN,
highlighting
an
indispensable
role
even
affluent
environment.
Further
cell
animal
studies
are
needed
deepen
our
understanding
underlying
mechanisms,
facilitating
drug
development.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: Sept. 11, 2024
Mitochondrial
quality
control
is
finely
tuned
by
mitophagy,
the
selective
degradation
of
mitochondria
through
autophagy,
and
mitochondrial
biogenesis.
Removal
damaged
essential
to
preserve
cellular
bioenergetics
prevent
detrimental
events
such
as
sustained
mitoROS
production,
pro-apoptotic
cytochrome
c
release
or
mtDNA
leakage.
The
array
tools
available
study
mitophagy
very
limited
but
in
constant
development.
Almost
a
decade
ago,
we
developed
method
assess
flux
using
MitoTracker
Deep
Red
combination
with
lysosomal
inhibitors.
Now,
novel
tandem-fluorescence
reporter
Zeitschrift für Komplementärmedizin,
Journal Year:
2024,
Volume and Issue:
16(04), P. 28 - 33
Published: July 1, 2024
Summary
In
der
Pathobiochemie
von
Neurodegeneration
und
Seneszenz
spielen
genomische
Instabilität,
Verkürzung
Telomere,
epigenetische
Mechanismen
sowie
reaktive
Sauerstoff-
Stickstoffspezies
eine
zentrale
Rolle.
Seneszente
Zellen
sind
charakteristisch
für
die
Alterung.
Sie
regulieren
antiapoptotische
Signalwege
verhindern
so
Absterben
Elimination
mit
Läsionen
an
DNA
Zellorganellen.
Altersforschung
stehen
Senolytika
im
Mittelpunkt,
Apoptose
oder
Lyse
seneszenter
induzieren
zudem
neuroprotektiv
wirken.
Das
senolytischeFlavonolFisetin
kommt
in
verschiedenen
Obst-
Gemüsesorten
vor
kann
seit
1904
synthetisiert
werden.
Es
schützt
Nervenzellen
Neurotoxizität,
Inflammation
apoptotischer
Degeneration;
Wirkung
muss
noch
durch
weitere
Studien
belegt
Auch
Senomorphika
wie
Metformin
werden
zur
Unterdrückung
eingesetzt.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: Sept. 20, 2024
Nicotinamide
adenine
dinucleotide
(NAD
+
)
is
crucial
for
cellular
energy
production,
serving
as
a
coenzyme
in
oxidation-reduction
reactions.
It
also
supports
enzymes
involved
processes
such
DNA
repair,
aging,
and
immune
responses.
Lower
NAD
levels
have
been
associated
with
various
diseases,
highlighting
the
importance
of
replenishing
NAD+.
phosphoribosyltransferase
(NAMPT)
plays
critical
role
salvage
pathway,
which
helps
sustain
levels,
particularly
high-energy
tissues
like
skeletal
muscle.This
review
explores
how
NAMPT-driven
pathway
influences
muscle
health
functionality
type
2
diabetes
mellitus
(T2DM),
injury.
The
offers
insights
into
enhancing
through
exercise
boosters
strategies
to
improve
performance.
findings
suggest
significant
potential
using
this
diagnosis,
monitoring,
treatment
conditions.
Cold Spring Harbor Perspectives in Medicine,
Journal Year:
2024,
Volume and Issue:
14(8), P. a041725 - a041725
Published: April 2, 2024
S.
Jay
Olshansky1
and
James
L.
Kirkland2
1School
of
Public
Health,
University
Illinois
at
Chicago,
60612,
USA
2Department
Physiology
Biomedical
Engineering,
Division
General
Internal
Medicine,
Mayo
Clinic,
Rochester,
Minnesota
55905,
Correspondence:
sjayo{at}uic.edu;
kirkland.james{at}mayo.edu
