NADH Reductive Stress and Its Correlation with Disease Severity in Leigh Syndrome: A Pilot Study Using Patient Fibroblasts and a Mouse Model DOI Creative Commons
Tamaki Ishima,

Natsuka Kimura,

Mizuki Kobayashi

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 15(1), P. 38 - 38

Published: Dec. 31, 2024

Nicotinamide adenine dinucleotide (NAD) is a critical cofactor in mitochondrial energy production. The NADH/NAD+ ratio, reflecting the balance between NADH (reduced) and NAD+ (oxidized), key marker for severity of diseases. We recently developed streamlined LC-MS/MS method precise measurement NAD+. Utilizing this technique, we quantified levels fibroblasts derived from pediatric patients Leigh syndrome mouse model which respiratory chain complex I subunit Ndufs4 knocked out (KO). In patient-derived fibroblasts, did not differ significantly those healthy controls (p = 0.79); however, were elevated 0.04), indicating increased reductive stress. This increase, observed despite comparable total NAD(H) groups, was attributed to levels. Similarly, model, KO group 0.002), further suggesting that elevation drives expected outperform conventional assays, such as lactate, providing simpler more reliable means assessing disease progression.

Language: Английский

Senolytika und Senomorphika im Kampf gegen neurodegenerative Erkrankungen DOI
Uwe Gröber,

Hans-Peter Friedrichsen

Zeitschrift für Orthomolekulare Medizin, Journal Year: 2024, Volume and Issue: 22(01), P. 4 - 10

Published: April 1, 2024

Zusammenfassung In der Pathobiochemie von Neurodegeneration und Seneszenz spielen genomische Instabilität, Verkürzung Telomere, epigenetische Mechanismen sowie reaktive Sauerstoff- Stickstoffspezies eine zentrale Rolle. Seneszente Zellen sind charakteristisch für die Alterung. Sie regulieren antiapoptotische Signalwege verhindern so Absterben Elimination mit Läsionen an DNA Zellorganellen. Altersforschung stehen Senolytika im Mittelpunkt, Apoptose oder Lyse seneszenter induzieren zudem neuroprotektiv wirken. Das senolytische Flavonol Fisetin kommt in verschiedenen Obst- Gemüsesorten vor kann seit 1904 synthetisiert werden. Es schützt Nervenzellen Neurotoxizität, Inflammation apoptotischer Degeneration; Wirkung muss noch durch weitere Studien belegt Auch Senomorphika wie Metformin werden zur Unterdrückung eingesetzt.

Citations

0
Markers of Mitochondrial Function and DNA Repair Associated with Physical Function in Centenarians DOI Creative Commons
Inés Sánchez-Román, Beatriz Ferrando,

Camilla Myrup Holst

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(8), P. 909 - 909

Published: July 26, 2024

Mitochondrial dysfunction and genomic instability are key hallmarks of aging. The aim this study was to evaluate whether maintenance physical capacities at very old age is associated with To investigate this, we measured mitochondrial bioenergetics, DNA (mtDNA) copy number repair capacity in peripheral blood mononuclear cells from centenarians. In addition, circulating levels NAD+/NADH, brain-derived neurotrophic factor (BDNF) carbonylated proteins were plasma these parameters correlated capacities. Centenarians without disabilities had lower respiration values including ATP production, reserve capacity, maximal non-mitochondrial oxygen-consumption rate higher mtDNA than centenarians moderate severe (

Language: Английский

Citations

0
Urolithin A and nicotinamide riboside differentially regulate innate immune defenses and metabolism in human microglial cells DOI Creative Commons
Helena Borland Madsen, Cláudia Maria Navarro,

Emilie Gasparini

et al.

Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16

Published: Nov. 27, 2024

During aging, many cellular processes, such as autophagic clearance, DNA repair, mitochondrial health, metabolism, nicotinamide adenine dinucleotide (NAD+) levels, and immunological responses, become compromised. Urolithin A (UA) Nicotinamide Riboside (NR) are two naturally occurring compounds known for their anti-inflammatory protective properties, yet the effects of these natural substances on microglia cells have not been thoroughly investigated. As both UA NR considered safe dietary supplements, it is equally important to understand function in normal disease states.

Language: Английский

Citations

0
The Role of NAD+ Metabolism in Cardiovascular Diseases: Mechanisms and Prospects DOI
Huimin Li, Qingxun Hu, Deqiu Zhu

et al.

American Journal of Cardiovascular Drugs, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

Citations

0
NADH Reductive Stress and Its Correlation with Disease Severity in Leigh Syndrome: A Pilot Study Using Patient Fibroblasts and a Mouse Model DOI Creative Commons
Tamaki Ishima,

Natsuka Kimura,

Mizuki Kobayashi

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 15(1), P. 38 - 38

Published: Dec. 31, 2024

Nicotinamide adenine dinucleotide (NAD) is a critical cofactor in mitochondrial energy production. The NADH/NAD+ ratio, reflecting the balance between NADH (reduced) and NAD+ (oxidized), key marker for severity of diseases. We recently developed streamlined LC-MS/MS method precise measurement NAD+. Utilizing this technique, we quantified levels fibroblasts derived from pediatric patients Leigh syndrome mouse model which respiratory chain complex I subunit Ndufs4 knocked out (KO). In patient-derived fibroblasts, did not differ significantly those healthy controls (p = 0.79); however, were elevated 0.04), indicating increased reductive stress. This increase, observed despite comparable total NAD(H) groups, was attributed to levels. Similarly, model, KO group 0.002), further suggesting that elevation drives expected outperform conventional assays, such as lactate, providing simpler more reliable means assessing disease progression.

Language: Английский

Citations

0