Natural compound Alternol actives multiple endoplasmic reticulum stress-responding pathways contributing to cell death DOI Creative Commons
Wang Liu, Chenchen He, Changlin Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 20, 2024

Background: Alternol is a small molecular compound isolated from the fermentation of mutant fungus obtained Taxus brevifolia bark. Our previous studies showed that treatment induced reactive oxygen species (ROS)-dependent immunogenic cell death. This study conducted comprehensive investigation to explore mechanisms involved in Alternol-induced Methods: Prostate cancer PC-3, C4-2, and 22RV1 were used this study. interaction with heat shock proteins (HSP) was determined using CETSA assay. Alternol-regulated ER stress assessed Western blot Extracellular adenosine triphosphate (ATP) measured ATPlite Luminescence Assay System. Results: results interacted multiple cellular chaperone increased their expression levels, including endoplasmic reticulum (ER) hypoxia up-regulated 1 (HYOU1) protein 90 alpha family class B member (HSP90AB1), as well cytosolic A 8 (HSPA8). These data represented potential cause unfolded response (UPR) after treatment. Further revealed triggered ROS-dependent responses via R-like kinase (PERK), inositol-requiring enzyme 1α (IRE1α). The double-stranded RNA-dependent (PKR) but not activating transcription factor 6 (ATF6) cascades, leading ATF-3/ATF-4 activation, C/EBP-homologous (CHOP) overexpression, X-box binding XBP1 splicing induction. In addition, inhibition these cascades blunted extracellular release, one classical hallmarks Conclusion: Taken together, our demonstrate

Language: Английский

The lipid side of unfolded protein response DOI Creative Commons
Wojciech Białek, Anita Hryniewicz‐Jankowska,

Paulina Czechowicz

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2024, Volume and Issue: 1869(7), P. 159515 - 159515

Published: June 5, 2024

Although our current knowledge of the molecular crosstalk between ER stress, unfolded protein response (UPR), and lipid homeostasis remains limited, there is increasing evidence that dysregulation either or profoundly affects other. Most research regarding UPR signaling in human diseases has focused on causes consequences disrupted folding. The itself consists very complex pathways function to not only maintain homeostasis, but just as importantly, modulate biogenesis allow adjust promote cell survival. Lipid known activate many aspects UPR, complexity this a major barrier. disequilibrium lipotoxicity are be important contributors numerous pathologies, including insulin resistance, liver disease, cardiovascular diseases, neurodegenerative cancer. Despite their medical significance continuous research, however, mechanisms synthesis during stress conditions, impact fate decisions, remain poorly understood. Here we summarize view connections altered metabolism, UPR.

Language: Английский

Citations

7

Aruncus Dioicus Var. Kamtschaticus Extract Prevents Ocular Endoplasmic Reticulum Stress, Inflammation, and Oxidative Stress In Vitro DOI
Sun‐Young Jeong, Miae Doo, K.L. Paul Sung

et al.

Journal of Medicinal Food, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

The aim of this study was to determine the anti-inflammatory and anti-endoplasmic reticulum (ER) stress effects Aruncus dioicus var. kamtschaticus (ADK) extract on ARPE-19 cells. Pretreatment with ADK effectively mitigated thapsigargin (Tg)-induced increases in vascular endothelial growth factor protein secretion intracellular calcium levels. Furthermore, pretreatment suppressed ocular ER stress-related expression a dose-dependent manner, inhibited loss tight junctions, interleukin-6 gene expression. Moreover, significantly prevented lipopolysaccharide-inducible proinflammatory cytokine at transcription level phosphorylation proteins involved mitogen-activated kinase-nuclear kappa-light-chain-enhancer activated B cells (NFκB) axis posttranslational level. Additionally, enhanced antioxidant activity, as evidenced by increased heme oxygenase-1 2,2-diphenyl-1-picrylhydrazyl radical scavenging ferric-reducing power. In conclusion, protected from stress, inflammation, oxidative demonstrating its potential nutraceutical intervention for diseases.

Language: Английский

Citations

0

Corticosterone effects induced by stress and immunity and inflammation: mechanisms of communication DOI Creative Commons

Jingyu Xu,

B Wang,

Haiqing Ao

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

The body instinctively responds to external stimuli by increasing energy metabolism and initiating immune responses upon receiving stress signals. Corticosterone (CORT), a glucocorticoid (GC) that regulates secretion along the hypothalamic-pituitary-adrenal (HPA) axis, mediates neurotransmission humoral regulation. Due widespread expression of receptors (GR), effects CORT are almost ubiquitous in various tissue cells. Therefore, on one hand, is molecular signal activates body’s system during other due chemical properties GCs, anti-inflammatory act as stabilizers control response stress. Inflammation manifestation activation. plays dual roles this process both promoting inflammation exerting As hormone, levels fluctuate with degree duration stress, determining its changes it induces. essential for resist diseases maintain homeostasis, imbalance being key factor development diseases. understanding role mechanisms action immunity crucial. This review addresses important issue summarizes interactions between system.

Language: Английский

Citations

0

Natural compound Alternol actives multiple endoplasmic reticulum stress-responding pathways contributing to cell death DOI Creative Commons
Wang Liu, Chenchen He, Changlin Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 20, 2024

Background: Alternol is a small molecular compound isolated from the fermentation of mutant fungus obtained Taxus brevifolia bark. Our previous studies showed that treatment induced reactive oxygen species (ROS)-dependent immunogenic cell death. This study conducted comprehensive investigation to explore mechanisms involved in Alternol-induced Methods: Prostate cancer PC-3, C4-2, and 22RV1 were used this study. interaction with heat shock proteins (HSP) was determined using CETSA assay. Alternol-regulated ER stress assessed Western blot Extracellular adenosine triphosphate (ATP) measured ATPlite Luminescence Assay System. Results: results interacted multiple cellular chaperone increased their expression levels, including endoplasmic reticulum (ER) hypoxia up-regulated 1 (HYOU1) protein 90 alpha family class B member (HSP90AB1), as well cytosolic A 8 (HSPA8). These data represented potential cause unfolded response (UPR) after treatment. Further revealed triggered ROS-dependent responses via R-like kinase (PERK), inositol-requiring enzyme 1α (IRE1α). The double-stranded RNA-dependent (PKR) but not activating transcription factor 6 (ATF6) cascades, leading ATF-3/ATF-4 activation, C/EBP-homologous (CHOP) overexpression, X-box binding XBP1 splicing induction. In addition, inhibition these cascades blunted extracellular release, one classical hallmarks Conclusion: Taken together, our demonstrate

Language: Английский

Citations

3