Melanocortin system activates carotid body arterial chemoreceptors in hypertension DOI Creative Commons
Audrys G. Pauža, Pratik Thakkar, Xin Shen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 25, 2024

Abstract Background The body’s internal milieu is controlled by a system of interoceptors coupled to motor outflows that drive compensatory adaptive responses. These include the arterial chemoreceptors, best known for sensing oxygen. In cardiometabolic diseases, such as essential hypertension, carotid bodies (CB) exhibit heightened reflex sensitivity and tonic activity without an apparent stimulus. mechanisms behind CB sensitization in these conditions are not well understood. Methods Guided functional genomics, range assays used interrogate downstream intracellular interorgan signalling pathways involved chemosensory function. Results Here, we report presence Melanocortin 4 receptor (MC4R) mammalian show its elevated expression experimental hypertension. We demonstrate melanocortin agonists activate cells, modulating afferent influence both resting chemoreflex-evoked sympathetic ventilatory activity. Transcriptional analysis hypertensive implicates activation Mash1 ( Ascl1 ) regulatory network driving Mc4r expression. Conclusions Collectively, our data indicate primarily pathophysiological role chemosensation, contributing excess disease.

Language: Английский

Pioneer factors: roles and their regulation in development DOI Creative Commons
Amandine Barral, Kenneth S. Zaret

Trends in Genetics, Journal Year: 2023, Volume and Issue: 40(2), P. 134 - 148

Published: Nov. 7, 2023

Language: Английский

Citations

36
Context-specific functions of chromatin remodellers in development and disease DOI
Sai Gourisankar, A. Krokhotin,

Wendy Wenderski

et al.

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 25(5), P. 340 - 361

Published: Nov. 24, 2023

Language: Английский

Citations

23
Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation DOI
Pratik Singh, Wei Yong Gu, Shariq Madha

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(7), P. 1038 - 1057.e11

Published: May 10, 2024

Language: Английский

Citations

5
SWI/SNF-type complexes–transcription factor interplay: a key regulatory interaction DOI Creative Commons
Anna Maassen, Jarosław Steciuk,

Magdalena Wilga

et al.

Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 10, 2025

Abstract ATP-dependent switch/sucrose nonfermenting-type chromatin remodeling complexes (SWI/SNF CRCs) are multiprotein machineries altering structure, thus controlling the accessibility of genomic DNA to various regulatory proteins including transcription factors (TFs). SWI/SNF CRCs highly evolutionarily conserved among eukaryotes. There three main subtypes CRCs: canonical (cBAF), polybromo (pBAF), and noncanonical (ncBAF) in humans their functional Arabidopsis counterparts SYD-associated (SAS), MINU-associated (MAS), BRAHMA (BRM)-associated (BAS). Here, we highlight importance interplay between TFs human summarize recent advances demonstrating role important processes. We discuss possible mechanisms involved CRCs-dependent transcriptional control gene expression. indicate that may serve as a valuable model for identification SWI/SNF–TF interactions postulate further exploration CRCs-interplay, especially context particular CRC subtypes, TF type, well cell/tissue conditions, others, will help address questions related specificity sequence events occurring on target genes. Graphical

Language: Английский

Citations

0
Transcription factor dynamics, oscillation, and functions in human enteroendocrine cell differentiation DOI Open Access
Pratik Singh, Wei Yong Gu, Shariq Madha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 9, 2024

Enteroendocrine cells (EECs), which secrete serotonin (enterochromaffin cells, EC) or a dominant peptide hormone, serve vital physiologic functions. As with any adult human lineage, the basis for terminal cell diversity remains obscure. We replicated EEC differentiation

Language: Английский

Citations

4
SWI/SNF Complex Connects Signaling and Epigenetic State in Cells of Nervous System DOI
Victor Chmykhalo, Roman V. Deev, Artemy T. Tokarev

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown

Published: July 13, 2024

Language: Английский

Citations

4
Pioneer factors outline chromatin architecture DOI Creative Commons

Juan Carlos Gómora-García,

Mayra Furlan-Magaril

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 93, P. 102480 - 102480

Published: Feb. 12, 2025

Language: Английский

Citations

0
Epigenetic control of cell identities from epiblast to gastrulation DOI Creative Commons
Katrin M. Schüle, Simone Probst

FEBS Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

Epigenetic modifications of chromatin are essential for the establishment cell identities during embryogenesis. Between embryonic days 3.5–7.5 murine development, major lineage decisions made that discriminate extraembryonic and tissues, primary germ layers formed, thereby laying down basic body plan. In this review, we cover contribution dynamic by DNA methylation, changes accessibility, histone modifications, in combination with transcription factors control gene expression programs different types. We highlight differences regulation enhancer promoter marks discuss their requirement specification. Importantly, many cases, lineage‐specific targeting epigenetic modifiers is carried out pioneer or master factors, sum mediate landscape cell‐type‐specific thus, identities.

Language: Английский

Citations

0
Viral-mediated Oct4 overexpression and inhibition of Notch signaling synergistically induce neurogenic competence in mammalian Müller glia DOI Open Access
Nguyet Le,

Sherine Awad,

Isabella Palazzo

et al.

Published: April 2, 2025

Retinal Müller glia in cold-blooded vertebrates can reprogram into neurogenic progenitors to replace neurons lost injury, but mammals lack this ability. While recent studies have shown that transgenic overexpression of bHLH factors and glial-specific disruption NFI family transcription Notch signaling induce competence mammalian glia, induction neurogenesis wildtype has thus far proven elusive. Here we report viral-mediated the pluripotency factor Oct4 ( Pou5f1 ) induces transdifferentiation mouse bipolar neurons, synergistically stimulates glial-derived parallel with loss function. Single cell multiomic analysis shows leads widespread changes gene expression chromatin accessibility, inducing activity both Rfx4 Yamanaka Sox2 Klf4. This study demonstrates retinal Muller identifying mechanisms could be used cell-based therapies for treating dystrophies.

Language: Английский

Citations

0
Viral-mediated Oct4 overexpression and inhibition of Notch signaling synergistically induce neurogenic competence in mammalian Müller glia DOI Open Access
Nguyet Le,

Sherine Awad,

Isabella Palazzo

et al.

Published: April 2, 2025

Retinal Müller glia in cold-blooded vertebrates can reprogram into neurogenic progenitors to replace neurons lost injury, but mammals lack this ability. While recent studies have shown that transgenic overexpression of bHLH factors and glial-specific disruption NFI family transcription Notch signaling induce competence mammalian glia, induction neurogenesis wildtype has thus far proven elusive. Here we report viral-mediated the pluripotency factor Oct4 ( Pou5f1 ) induces transdifferentiation mouse bipolar neurons, synergistically stimulates glial-derived parallel with loss function. Single cell multiomic analysis shows leads widespread changes gene expression chromatin accessibility, inducing activity both Rfx4 Yamanaka Sox2 Klf4. This study demonstrates retinal Muller identifying mechanisms could be used cell-based therapies for treating dystrophies.

Language: Английский

Citations

0