De Novo Long-Read Genome Assembly and Annotation of the Luna Moth (Actias luna) Fully Resolves Repeat-Rich Silk Genes DOI Creative Commons
Amanda Markee, R. Keating Godfrey, Paul B. Frandsen

et al.

Genome Biology and Evolution, Journal Year: 2024, Volume and Issue: 16(7)

Published: July 1, 2024

Abstract We present the first long-read de novo assembly and annotation of luna moth (Actias luna) provide full characterization heavy chain fibroin (h-fibroin), a long highly repetitive gene (>20 kb) essential in silk fiber production. There are >160,000 described species moths butterflies (Lepidoptera), but only within last 5 years have we begun to recover high-quality annotated whole genomes across order that capture h-fibroin. Using PacBio HiFi reads, produce reference genome for this species. The assembled has length 532 Mb, contig N50 16.8 an L50 14 contigs, 99.4% completeness (BUSCO). Our using Bombyx mori protein A. RNAseq evidence captured total 20,866 genes at 98.9% with 10,267 functionally proteins full-length h-fibroin 2,679 amino acid residues.

Language: Английский

SGDAcn is a suppressor for silk gland endoreplication and development DOI Open Access
Lin Wu, Li Zhao, Yuting Feng

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract Silkworm silk gland cells undergo multiple rounds of endoreplication, a process in which the genome is duplicated without cell division, leading to cellular polyploidization. This results accumulation genomic DNA, serving as foundation for rapid proteins synthesis. For first time, we report previously uncharacterized gene, SGDAcn , silkworm that clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR‐associated nuclease 9‐mediated knockout posterior increased size and enhanced production. facilitated progression endoreplication by upregulating expression various cyclin genes promoting energy metabolism, substantial increase fibroin gene its transcription factor Dimm well stimulation ribosome biogenesis messenger RNA translation enhancement eukaryotic initiation factors protein Our findings demonstrate influences growth, nucleolus through ‐EGFR/PI3K/AKT ‐NF‐ κ B signaling pathways. Overall, acts negative regulator development, affecting synthesis, thus modulating These mechanisms might be conserved growth progression, making an attractive target genetic editing improve yield silkworms potentially mammalian regulation.

Language: Английский

Citations

0
High throughput CRISPR approaches: New solutions for challenging problems DOI

Gargi Bindal,

Chitra Seetharam Misra,

Mandeep Singh

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118245 - 118245

Published: March 1, 2025

Language: Английский

Citations

0
De Novo Long-Read Genome Assembly and Annotation of the Luna Moth (Actias luna) Fully Resolves Repeat-Rich Silk Genes DOI Creative Commons
Amanda Markee, R. Keating Godfrey, Paul B. Frandsen

et al.

Genome Biology and Evolution, Journal Year: 2024, Volume and Issue: 16(7)

Published: July 1, 2024

Abstract We present the first long-read de novo assembly and annotation of luna moth (Actias luna) provide full characterization heavy chain fibroin (h-fibroin), a long highly repetitive gene (>20 kb) essential in silk fiber production. There are >160,000 described species moths butterflies (Lepidoptera), but only within last 5 years have we begun to recover high-quality annotated whole genomes across order that capture h-fibroin. Using PacBio HiFi reads, produce reference genome for this species. The assembled has length 532 Mb, contig N50 16.8 an L50 14 contigs, 99.4% completeness (BUSCO). Our using Bombyx mori protein A. RNAseq evidence captured total 20,866 genes at 98.9% with 10,267 functionally proteins full-length h-fibroin 2,679 amino acid residues.

Language: Английский

Citations

1