Phase boundaries promote chemical reactions through localized fluxes
The Journal of Chemical Physics,
Journal Year:
2025,
Volume and Issue:
162(2)
Published: Jan. 9, 2025
One
of
the
hypothesized
functions
biomolecular
condensates
is
to
act
as
chemical
reactors,
where
reactions
can
be
modulated,
i.e.,
accelerated
or
slowed
down,
while
substrate
molecules
enter
and
products
exit
from
condensate.
Similarly,
components
themselves
that
take
part
in
architectural
integrity
might
modified
by
active
(energy
consuming,
non-equilibrium)
processes,
e.g.,
ATPase
chaperones
kinases
phosphatases.
In
this
work,
we
study
how
presence
spatial
inhomogeneities,
such
case
liquid–liquid
phase
separation,
affects
results
directional
flows
matter,
which
are
one
hallmarks
non-equilibrium
processes.
We
establish
minimal
conditions
for
existence
currents,
furthermore
find
these
fluxes
maximal
at
condensate
interface.
These
propose
some
most
efficient
factories
due
their
interfaces
rather
than
volumes
could
suggest
a
possible
biological
reason
observed
abundance
small
non-fusing
inside
cell,
thus
maximizing
surface
associated
fluxes.
Language: Английский
Principles of metabolic pathway control by biomolecular condensates in cells
Nature Chemical Engineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 12, 2025
Language: Английский
The mechanobiology of biomolecular condensates
Biophysics Reviews,
Journal Year:
2025,
Volume and Issue:
6(1)
Published: March 1, 2025
The
central
goal
of
mechanobiology
is
to
understand
how
the
mechanical
forces
and
material
properties
organelles,
cells,
tissues
influence
biological
processes
functions.
Since
first
description
biomolecular
condensates,
it
was
hypothesized
that
they
obtain
are
tuned
their
functions
inside
cells.
Thus,
represent
an
intriguing
playground
for
mechanobiology.
idea
condensates
exhibit
diverse
adaptive
highlights
need
different
states
respond
external
whether
these
responses
linked
physiological
roles
within
cell.
For
example,
liquids
buffer
dissipate,
while
solids
store
transmit
stress,
relaxation
time
a
viscoelastic
can
act
as
frequency
filter.
Hence,
liquid-solid
transition
condensate
in
force
transmission
pathway
determine
signals
transduced
in-between
affecting
differentiation,
neuronal
network
dynamics,
behavior
stimuli.
Here,
we
review
our
current
understanding
molecular
drivers
rigidity
phase
transitions
set
forth
complex
cellular
environment.
We
will
then
summarize
technical
advancements
were
necessary
insights
into
rich
fascinating
finally,
highlight
recent
examples
connection
specific
Our
provide
comprehensive
summary
field
on
cells
harness
regulate
mechanics
achieve
Language: Английский
Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
26(1), P. 363 - 373
Published: Dec. 2, 2024
Accurate
determination
of
biomolecular
condensate
volume
reveals
that
destabilization
condensates
can
lead
to
either
swelling
or
shrinking
condensates,
giving
fundamental
insights
into
the
regulation
cellular
condensates.
Determination
and
coacervate
protocells
is
essential
investigate
their
precise
composition
impact
on
(bio)chemical
reactions
are
localized
inside
It
not
a
straightforward
task,
as
have
tiny
volumes,
highly
viscous,
prone
wetting.
Here,
we
examine
different
strategies
determine
introduce
two
new
methods,
with
which
volumes
1
μL
less
(volume
fraction
0.4%)
be
determined
standard
deviation
0.03
μL.
Using
these
show
depends
degree
physical
cross-linking.
These
observations
supported
by
Flory–Huggins
theory
profound
effects
in
cell
biology.
Language: Английский