Evaluation of the prognostic value of lncRNA UCA1 combined with extravascular lung water index and lung ultrasound score in patients with acute lung injury DOI Creative Commons

Zhao-Peng Jiang,

Jiaqi Wu,

Ming Wan

et al.

The Clinical Respiratory Journal, Journal Year: 2023, Volume and Issue: 17(7), P. 654 - 662

Published: June 15, 2023

Acute lung injury (ALI) is a common and rapidly developing critical inflammatory disease in clinic. This study investigated the predictive value of lncRNA UCA1, extravascular water index (EVLWI), ultrasound score (LUS) predicting overall outcome patients with ALI.Patients ALI were recruited for detecting content EVLWI, LUS. All cataloged into survival group death according to prognosis. The discrepancy LUS was compared two groups. prognostic significance LUS, their combination estimated by logistic regression receiver operating characteristic (ROC) curve.The levels EVLWI elevated group. UCA1 positively correlated scores scores. independent indicators prognosis ALI. ROC curve reflected that could forecast endpoint events whereas combined approach had highest accuracy.Highly expressed biomarker forecasting It high accuracy when EVLWI.

Language: Английский

The Role of lncRNAs in Sepsis-Induced Acute Lung Injury: Molecular Mechanisms and Therapeutic Potential DOI
Haisong Qi,

Ying Gu,

Ling Wang

et al.

Archives of Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown, P. 110407 - 110407

Published: April 1, 2025

Language: Английский

Citations

0
Regulation of mitochondrial autophagy by lncRNA MALAT1 in sepsis-induced myocardial injury DOI Creative Commons

Guangqing Huang,

Xu Zhao, Yong Bai

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Nov. 1, 2024

Sepsis-induced myocardial injury (SIMI) is a severe complication of sepsis, contributing significantly to mortality. Mitochondrial dysfunction and dysregulated autophagy are implicated in SIMI pathogenesis. Long non-coding RNA MALAT1 has been associated with various diseases, including but its role remains unclear.

Language: Английский

Citations

2
Circulating Exosomes from Septic Mice Activate NF-κB/MIR17HG Pathway in Macrophages DOI Creative Commons
Shao‐Chun Wu,

Cheng‐Shyuan Rau,

Yi-Chan Wu

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 534 - 534

Published: Feb. 27, 2024

Circulating exosomes derived from polymicrobial sepsis contain various non-coding RNAs and proteins. Isobaric tags for a relative or absolute quantitation proteomic analysis of the exosomal content revealed 70 dysregulated proteins in circulating septic mice. Next-generation sequencing was used to profile long RNA expression primary cultured macrophages treated with obtained blood C57BL/6 mice, it discovered that nuclear factor-kappa B (NF-κB)/miR-17-92a-1 cluster host gene (MIR17HG) pathways were activated macrophages. The inhibition MIR17HG by interference resulted significantly decreased cell viability. pull-down assays ten protein targets bind MIR17HG. Interaction networks pulled down constructed using GeneMANIA, their functions mainly involved ribonucleoprotein granules, type I interferons, regulation organelle assembly, biosynthesis acetyl coenzyme A, as signal transducer activator transcription (STAT) phosphorylation, mRNA splicing. Furthermore, inhibited expression, resulting survival. In conclusion, this work considerable overexpression sepsis-affected animals. This study’s findings assist us comprehending role modulating inflammatory responses mediating pathogenic during sepsis.

Language: Английский

Citations

1
Down‐regulating lncRNA KCNQ1OT1 relieves type II alveolar epithelial cell apoptosis during one‐lung ventilation via modulating miR‐129‐5p/HMGB1 axis induced pulmonary endothelial glycocalyx DOI
Zhenghuan Song, Zhongqiu Wang, Jiaqin Cai

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(6), P. 3578 - 3596

Published: March 15, 2024

Endothelial glycocalyx (EG) maintains vascular homeostasis and is destroyed after one-lung ventilation (OLV)-induced lung injury. Long noncoding RNAs (lncRNAs) are critically involved in various injuries. This study aimed to investigate the role regulatory mechanism of KCNQ1 overlapping transcript 1 (KCNQ1OT1) OLV-induced injury LPS-induced type II alveolar epithelial cell (AECII) apoptosis.

Language: Английский

Citations

1
MiR-98-3p alleviates lipopolysaccharide-induced pulmonary microvascular endothelial barrier dysfunction by targeting DKK3 in sepsis-induced acute lung injury DOI Open Access
Dan Zhong, Cong Luo, Neng Wang

et al.

The Journal of Toxicological Sciences, Journal Year: 2024, Volume and Issue: 49(7), P. 289 - 299

Published: Jan. 1, 2024

Background: Endothelial barrier dysfunction is critical for the pathogenesis of sepsis-induced acute lung injury (ALI). Lipopolysaccharide (LPS)-stimulated human pulmonary microvascular endothelial cells (HPMECs) are widely used as cell model sepsis-associated ALI exploration dysfunction. Dickkopf (DKK) family proteins were reported to mediate functions in various diseases. The present study explored effect Dickkopf-3 (DKK3) on permeability, angiogenesis, and tight junctions LPS-stimulated HPMECs. Methods: RT-qPCR was required detecting DKK3 miR-98-3p expression. angiogenesis HPMECs evaluated by tube formation assays. Monolayer permeability examined Transwell rhodamine protein expression measured via western blotting. Luciferase reporter assay verify interaction between DKK3. Results: LPS treatment inhibited angiogenetic ability while increasing upregulated level reduced LPS-treated knockdown alleviated HPMEC triggered stimulation. MiR-98-3p targeted Overexpression protects from LPS-induced dysfunction, protective reversed overexpression. Conclusions: ameliorates LPS-evoked targeting

Language: Английский

Citations

1
Evaluation of the prognostic value of lncRNA UCA1 combined with extravascular lung water index and lung ultrasound score in patients with acute lung injury DOI Creative Commons

Zhao-Peng Jiang,

Jiaqi Wu,

Ming Wan

et al.

The Clinical Respiratory Journal, Journal Year: 2023, Volume and Issue: 17(7), P. 654 - 662

Published: June 15, 2023

Acute lung injury (ALI) is a common and rapidly developing critical inflammatory disease in clinic. This study investigated the predictive value of lncRNA UCA1, extravascular water index (EVLWI), ultrasound score (LUS) predicting overall outcome patients with ALI.Patients ALI were recruited for detecting content EVLWI, LUS. All cataloged into survival group death according to prognosis. The discrepancy LUS was compared two groups. prognostic significance LUS, their combination estimated by logistic regression receiver operating characteristic (ROC) curve.The levels EVLWI elevated group. UCA1 positively correlated scores scores. independent indicators prognosis ALI. ROC curve reflected that could forecast endpoint events whereas combined approach had highest accuracy.Highly expressed biomarker forecasting It high accuracy when EVLWI.

Language: Английский

Citations

2