Highly Efficient Production of Ergothioneine from Glycerol through Gene Mining and CRISPR-Cas9 Synthetic Biology Technology in Saccharomyces cerevisiae DOI

Yinghao Yu,

Hanqing Liu, Shan Zhang

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(43), P. 15901 - 15912

Published: Oct. 17, 2024

Language: Английский

Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana DOI
Clement Kiing Fook Wong, Noor Baity Saidi, Ganesan Vadamalai

et al.

Journal of Applied Microbiology, Journal Year: 2019, Volume and Issue: 127(2), P. 544 - 555

Published: May 11, 2019

This study sought to investigate the effect of bioformulation on biocontrol efficacy, microbial viability and storage stability a consortium Pseudomonas aeruginosa DRB1 Trichoderma harzianum CBF2 against Foc Tropical Race 4 (Foc-TR4).Four bioformulations consisting dry (pesta granules, talc powder alginate beads) liquid formulations were evaluated for their ability control Foc-TR4, sustain populations after application maintain during storage. All tested reduced disease severity (DS) by more than 43·00% with pesta granules producing highest reduction in DS 66·67% lowest area under progress curve value (468·75) glasshouse trial. Microbial abundant rhizosphere, rhizoplane within roots bananas as compared powder, beads 84 days inoculation (DAI). The both 180 at 4°C was greatest granule formulation.The formulation suitable carrier biological agents (BCA) without compromising population other used this study.Pesta could be utilized formulate BCA consortia into biofertilizers. further investigated possible applications agricultural field settings.

Language: Английский

Citations

56

Production of lipids and carotenoids by Rhodotorula gracilis ATCC 10788 yeast in a bioreactor using low-cost wastes DOI
Anna M. Kot, Stanisław Błażejak, Marek Kieliszek

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2020, Volume and Issue: 26, P. 101634 - 101634

Published: May 4, 2020

Language: Английский

Citations

56

Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria DOI Creative Commons
Chao Zhong, Christina Ukowitz, Konrad J. Domig

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2020, Volume and Issue: 68(32), P. 8557 - 8567

Published: July 20, 2020

Short-chain cello-oligosaccharides (COS; degree of polymerization, DP ≤ 6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show the intensification scale up (20 mL; gram scale) COS production 93 g/L product in 82 mol % yield (0.5 M). The were comprised 3 (33 wt %), 4 (34 5 (24 6 (9 %) involved minimal loss (≤10 insoluble fractions. After isolation (≥95% purity; ≥90% yield), examined for growth promotion probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides, cellobiose, showed 4.1-fold stimulation cell density Clostridium butyricum, Lactococcus lactis subsp. lactis, Lactobacillus paracasei paracasei, rhamnosus but less with Bifidobacterium sp. This study shows as selectively functional carbohydrates prebiotic potential demonstrates enzymatic production.

Language: Английский

Citations

55

Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues DOI Creative Commons
Marta Mota,

Paula Múgica,

Isabel Sá‐Correia

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(7), P. 687 - 687

Published: June 29, 2022

Exploration of yeast diversity for the sustainable production biofuels, in particular biodiesel, is gaining momentum recent years. However, sustainable, and economically viable bioprocesses require strains exhibiting: (i) high tolerance to multiple bioprocess-related stresses, including various chemical inhibitors present hydrolysates from lignocellulosic biomass residues; (ii) ability efficiently consume all major carbon sources present; (iii) capacity produce lipids with adequate composition yields. More than 160 non-conventional (non-

Language: Английский

Citations

30

Bioleaching for metals removal from mine tailings flotation fractions DOI Creative Commons
Tamara A. Schueler, Axel Schippers, Daniel Goldmann

et al.

Hydrometallurgy, Journal Year: 2024, Volume and Issue: 225, P. 106286 - 106286

Published: Feb. 20, 2024

This study investigated two bioleaching strategies for removing heavy metals from three mine tailings fractions generated by flotation processes. On the one hand, with microbial consortia of acidophilic mesophiles and moderate thermophiles efficiently extracted Co, Cu, Zn, As, while leaching Pb was facilitated through use organic acids produced a heterotrophic bacterium fungus. Approximately 100% 68% 63% 31% Cu were bioleached barite (BT) sample after 14 days, whereas concentrate (BC) results showed about 70% Zn 45% removal at same period. The sulfide (SC) underwent both consortia, over 28 days. Approximately, 67% 28% 56% 6% Mn using mesophiles, efficiency 72% 50% 36% 5% in 20 L bioreactors. other explored Gluconobacter oxydans fungus Penicillium simplicissimum production gluconic acid citric acid, respectively. Additionally, besides glucose-based media, glycerol crystal sugar tested as alternative cheaper carbon sources. metabolic activity P. allowed maximum 39–43% BT days glycerol-based medium, BC sample, extraction around 60% medium. A lower achieved G. samples. 34% 39% reached within 7 when glucose used source. Further optimization should address enhancement – especially reduction costs related to media formulation fungal biomass on larger scale.

Language: Английский

Citations

9

Reuterin, Phenyllactic Acid, and Exopolysaccharides as Main Antifungal Molecules Produced by Lactic Acid Bacteria: A Scoping Review DOI Creative Commons
Andrea Ponzio, Annalisa Rebecchi,

Rosanna Zivoli

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(5), P. 752 - 752

Published: Feb. 29, 2024

The primary goal of this scoping review is to collect, analyze, and critically describe information regarding the role main compounds (reuterin, phenyllactic acid, exopolysaccharides) produced by LAB that possess antifungal properties provide some suggestions for further research. use lactic acid bacteria (LAB) mitigate spoilage extend shelf life foodstuffs has a long history. Recently, there been growing interest in unique these additions which they are applied. In recent studies biopreservation, significant attention given microorganisms their metabolites. This fascinating discipline aims not only replace traditional preservation systems, but also improve overall quality final product. biologically active by-products synthesized under certain conditions (time, temperature, aerobiosis, acidity, water activity, etc.), can be enacted through one oldest approaches food processing: fermentation (commonly used dairy bakery sectors). study delves into biosynthetic pathways synthesized, with particular emphasis on what known about mechanisms action against molds relation type food.

Language: Английский

Citations

7

Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose DOI
Frédéric Carly, Sébastien Steels,

Samuel Telek

et al.

Bioresource Technology, Journal Year: 2017, Volume and Issue: 247, P. 963 - 969

Published: Sept. 28, 2017

Language: Английский

Citations

50

Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges DOI Creative Commons
Luís C. Martins, Catarina C. Monteiro,

Paula M. Semedo

et al.

Applied Microbiology and Biotechnology, Journal Year: 2020, Volume and Issue: 104(15), P. 6527 - 6547

Published: May 30, 2020

Abstract Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to implementation efficient bioprocesses however manyfold thoroughly discussed this review paper, mainly at biological level. most important yeast cell factory platform advanced biorefineries is currently Saccharomyces cerevisiae , but species cannot naturally catabolise main sugars present pectin-rich hydrolysates, particular d -galacturonic acid l -arabinose. However, there non- (non-conventional yeasts) considered advantageous alternatives whenever they can express highly interesting metabolic pathways, natively assimilate a wider range carbon sources or exhibit higher tolerance relevant bioprocess-related stresses. For reason, interest non-conventional yeasts biomass-based gaining momentum. This paper focuses on valorisation by exploring that vast versatility use substrates their hydrolysates robustness cope multiple stresses encountered. major progresses made related isolation, selection, sugar catabolism, engineering S. -derived strains bioconversion residue discussed. reported examples value-added products synthesised different using reviewed. Key Points • Review yeasts. Catabolic pathways hydrolysates. Multiple affect productivity. Yeast improve bioconversion.

Language: Английский

Citations

48

Successful biosynthesis of natural antioxidant ergothioneine in Saccharomyces cerevisiae required only two genes from Grifola frondosa DOI Creative Commons
Yinghao Yu, Hong-Yu Pan, Li‐Qiong Guo

et al.

Microbial Cell Factories, Journal Year: 2020, Volume and Issue: 19(1)

Published: Aug. 18, 2020

Abstract Background Ergothioneine (EGT) has a unique antioxidant ability and diverse beneficial effects on human health. But the content of EGT is very low in its natural producing organisms such as Mycobacterium smegmatis mushrooms. Therefore, it necessary to highly efficient heterologous production food-grade yeasts Saccharomyces cerevisiae . Results Two biosynthetic genes were cloned from mushroom Grifola frondosa successfully heterologously expressed EC1118 strain this study. By optimization fermentation conditions engineered S. EC1118, 11.80 mg/L was obtained. With daily addition 1% glycerol culture medium process, can reach up 20.61 mg/L. Conclusion A successful de novo system containing only two developed This provides promising prospects for large scales

Language: Английский

Citations

41

What makes Komagataella phaffii non-conventional? DOI Creative Commons
Özge Ata, Burcu Gündüz Ergün, Patrick Fickers

et al.

FEMS Yeast Research, Journal Year: 2021, Volume and Issue: 21(8)

Published: Nov. 23, 2021

The important industrial protein production host Komagataella phaffii (syn Pichia pastoris) is classified as a non-conventional yeast. But what exactly makes K. non-conventional? In this review, we set out to address the main differences 'conventional' yeast Saccharomyces cerevisiae, but also pinpoint other yeasts used in biotechnology. Apart from its methylotrophic lifestyle, Crabtree-negative species. even within methylotrophs, possesses distinct regulatory features such glycerol-repression of methanol-utilization pathway or lack nitrate assimilation. Rewiring transcriptional networks regulating carbon (and nitrogen) source utilization clearly contributes our understanding genetic events occurring during evolution mechanisms mating-type switching and triggers morphogenic phenotypes represent further examples for how distinguished model S. cerevisiae. With respect heterologous production, high secretory capacity secretes only low amounts endogenous proteins. Different Golgi apparatus stacked like mammals. While it tempting speculate that architecture correlated secretion levels different N-glycan structures observed phaffii, there recent evidence against this. We conclude with unique has lot potential explore both fundamental research questions applications.

Language: Английский

Citations

36