Culturable Plastisphere from the 75° N Subarctic Transect as a Potential Vector of Pathogens and Antibiotic-Resistant Bacteria

Journal of Marine Science and Engineering, Journal Year: 2025, Volume and Issue: 13(3), P. 448 - 448

Published: Feb. 26, 2025

Plastic pollution is a global emerging concern, but in the Arctic Ocean, role of plastisphere as potential carrier pathogens and antibiotic-resistant bacteria unknown yet. An initial assessment spread these target through their colonization plastic particles, attributed to micro-sized fraction (less than 5 mm, named microplastics, MPs), was carried out across 75° N transect (Greenland Sea). To fill knowledge gaps regarding bacterial community associated withmicroplastics (MPs)—belonging so-called “plastisphere”—and risks related spread, our study focused on abundance taxonomic composition plastisphere, including pathogenic bacteria, using culture-dependent approach. MPs particles were collected Manta net, decimal dilutions cultured Marine agar plates estimate culturable heterotrophic bacteria. For search species (Escherichia coli, Enterococcus spp., Salmonella potentially Vibrio Staphylococcus aureus), small volumes inoculated into selective culture media aspread plate directly or after enrichment. Screening antibiotic susceptibility profiles isolates performed assess presence The dominated by members phyla Gammaproteobacteria Actinobacteria, with assigned genera Psychrobacter, Pseudoalteromonas, Shewanella, Arthrobacter. Selective enrichments resulted detection pathogens, mostly identified Vibrios examined samples. pointed that multiple also isolated, suggesting need shed light human animal health deriving from remote cold regions well.

Language: Английский

Beyond blooms: A novel time series analysis framework predicts seasonal keystone species and sheds light on Arctic pelagic ecosystem stability

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

ABSTRACT A thorough understanding of ecosystem functioning in the Arctic Ocean, a region under severe threat by climate change, requires detailed studies on linkages between biodiversity and stability. The identification keystone species with special relevance for stability is great importance, yet difficult to achieve established community assessments. In case microbes, metabarcoding metagenomics offer fundamental insights into structure function, remain limited regarding ecological individual taxa. To overcome this limitation, we have developed an analytical approach based three different methods: Co-Occurrence Networks, Convergent Cross Mapping, Energy Landscape Analysis. These methods enable seasonal communities microbial ecosystems, elucidate their interactions, predict potential stable configurations varying environmental conditions. Combining outcomes these allowed us define 38 Fram Strait that represent trophic modes within food web, might signify indicator functionality impact change. Our research reveals clear pattern phytoplankton composition, distinct assemblages characterizing phases carbon fixation (polar day) consumption night). Species interactions exhibited strong seasonality, significant influence summer winter but not vice versa. Spring harbored two groups: consumers (heterotrophs), strongly linked polar night, photoautotrophs (mainly Bacillariophyta). groups are causally related, suggesting “winter reset” selective effects facilitates new blooming period, allowing survivors dark phase emerge. Analysis showed more than communities. summary, landscape can be categorized phases: production governed specialized organisms highly responsive variability, heterotrophic dominated generalist enhanced resilience.

Language: Английский

Beyond blooms: the winter ecosystem reset determines microeukaryotic community dynamics in the Fram Strait

Communications Earth & Environment, Journal Year: 2024, Volume and Issue: 5(1)

Published: Oct. 29, 2024

Abstract The Arctic Ocean is undergoing a major transition as result of global warming, with uncertain consequences for its ecosystems. Our study introduces an integrated analytical approach using co-occurrence networks, convergent cross-mapping, and energy landscape analysis. Applied to four years amplicon data from Fram Strait, located at the boundary between Atlantic waters, our method identifies keystone species in seasonal microbial communities, elucidates causal interactions, predicts stable configurations across changing environments. We find strong evidence “winter reset”, implying that organisms representing spring bloom are largely determined by prevailing environmental conditions during winter. In addition, analysis suggests winter communities may adapt more readily expected Atlantification than summer communities. These results highlight utility innovative time-series analyses disentangling ecosystem dynamics. This provides critical insights into ecological dynamics, resilience aids understanding responses change.

Language: Английский