Insights into putative alginate lyases from epipelagic and mesopelagic communities of the global ocean

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 8, 2025

Alginate lyases and oligoalginate catalyze the cleavage of glycosidic bonds alginate, an acidic polysaccharide synthesized by brown algae other organisms. These enzymes are highly diverse, currently classified into 15 families Carbohydrate-Active Enzyme (CAZy) database. We explored structural taxonomic diversity, biogeographic distribution genes transcripts, potential environmental drivers putative alginate-degrading from picoplanktonic communities upper layers global ocean. The identified sequences were first analyzed using sequence similarity networks to assess their relationship with CAZy members. Sequences related PL5, PL6, PL7, PL17, PL38 had higher gene transcript abundances, temperature being a key driver structuring community members carrying alginate lyase genes. PL5 homologs included variants in residue active site, assigned 'Candidatus Pelagibacter' showed high abundances that negatively correlated inorganic phosphorus concentrations. Flavobacteriia and/or Gammaproteobacteria classes dominated PL17 families, particular those closely uncultured Polaribacter Alteromonas australica. In family, while taxa Planctomycetota, Verrucomicrobiota, Bacteroidota phyla highest relative abundance at most regions depths, expression levels observed latitudes Eukaryota (e.g., Phaeocystis antarctica). Overall, uncovered this study could be involved various physiological processes, including assimilation biosynthesis.

Language: Английский

Insights into the seasonal changes in the taxonomic and functional diversity of bacteria in the eastern Arabian Sea: Shotgun metagenomics approach

Marine Environmental Research, Journal Year: 2024, Volume and Issue: 199, P. 106616 - 106616

Published: June 18, 2024

Language: Английский