bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 13, 2023
Abstract
Cultivated
meat,
a
sustainable
alternative
to
traditional
livestock
farming,
has
gained
attention
for
its
potential
environmental
and
health
benefits.
However,
concerns
about
microplastic
contamination
pose
challenges,
especially
when
sourcing
cells
from
marine
organisms
prone
bioaccumulation.
Additionally,
the
pervasive
presence
of
microplastics
in
laboratory
settings,
ingredients,
during
production,
increases
risk
unintentional
contamination.
This
study
focused
on
Atlantic
mackerel
(
Scomber
scombrus
)
skeletal
muscle
cell
lines
examine
effects
exposure,
represented
by
fluorescent
polyethylene
microspheres
(10-45
µm)
performance
including
proliferation,
viability,
gene
expression,
differentiation
processes
critical
cultivated
meat
production.
The
results
revealed
significant
impacts
attachment
proliferation
at
concentrations
1
µg/mL,
10
50
µg/mL.
Notably,
µg/mL
concentration
exerted
most
pronounced
viability
both
phases.
While
indicated
that
size
influence
remained
unaffected,
additional
contributing
factors
require
further
investigation.
These
findings
underscore
necessity
thoroughly
exploring
microplastic-cell
interactions
ensure
food
safety
safeguard
within
burgeoning
industry.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 17, 2023
Abstract
The
evolution
of
the
circular
bioeconomy
will
require
realisation
new
value
from
waste
streams
generated
all
manufacturing
processes.
Bioprocessing
recombinant
proteins
and
functional
foods
using
animal
microbial
fermentations
are
fast
growing
industries
with
increasing
volumes
spent
culture
media
resulting
in
significant
resource
inefficiency.
Here
Chinese
hamster
ovary
cell
(CSM)
Trametes
versicolor
fungal
(FSM)
were
used
as
model
to
feed
Escherichia
coli
secondary
fermentations.
Expression
a
fusion
protein
was
measure
these
feedstuff.
E.
cultures
fed
either
stream
bioreactors
produced
equivalent
yields
those
rich
microbiological
media.
Separately
CSM
tested
replacement
for
fermentation
producing
beta
alpha
glucans.
This
waste-fed
resulted
2-fold
higher
glucan
yield
than
when
standard
corn
steep
liquor
feed.
Quantitative
analysis
content
mass
spectrometry
hplc
methods
showed
differences
elemental
composition
profiles,
quantities
individual
amino
acids
carboxylic
levels
carbon
sources.
These
findings
underline
versatility
E.coli
utilisation
feedstuff
show
that
range
applications
CHO
not
limited
feeding
bacterial
fermentation.
Exploration
cellular
bioprocesses
can
efficiently
use
be
valuable
step
towards
increased
efficiency
bioprocessing
industries.
(249/250).
Significance
Statement
expanding
scale
biomolecular
products
developed
activities
cells
is
responsible
production
hundreds
millions
litres
annually.
Recycling
this
has
potential
decrease
impact
associated
high
water
energy
consumption
contributing
meeting
targets
Paris
2050
Agreement
UN
Sustainable
Development
Goals.
work
shows
distinct
reused
sustain
high-level
notable
finding
add
process
by
its
productivity.
Efficient
valorisation
an
urgent
scientifically
objective.
(120/120)
