Listeria monocytogenes Challenge Testing of Ready-to-Eat Uncured Vacuum-Packed Pork Bars with Dried Plasma DOI Creative Commons
Paweł Pniewski, Krzysztof Anusz, Michał Tracz

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(24), P. 12055 - 12055

Published: Dec. 23, 2024

Newly developed formulas of ready-to-eat (RTE) products, despite conforming to the latest nutritional trends involving low-processed and high-protein may pose a risk promoting growth Listeria monocytogenes during shelf life. Food safety experts recommend challenge tests assess potential L. monocytogenes, which will ultimately provide microbiological evidence determine food limit. The present study evaluated whether RTE uncured vacuum-packed pork bars with dried plasma met 100 CFU/g level at end life under certain storage conditions aimed develop predictive models for in above product. were artificially inoculated mixture three strains two different inoculum densities 2 log 5 stored temperatures (2, 4, 6 °C) then subjected evaluation specific time intervals up 21 days. (Δ-value) contaminated was 0.36, 0.14, 0.91 2, °C, respectively, while inoculum, they −0.36, −0.40, 0.64 respectively. Statistically higher (p < 0.05) detected than CFU/g. results indicate that this type product must be classified as category: “Ready-to-eat foods able support other those intended infants special medical purposes” (Category 1.2. according EU Regulation 2073/2005). newly created can also describe an environment where factors, such temperature, pH, aw, change time. showed density statistically reduced values compared lower density.

Language: Английский

Studying Heat Stress Adaptation of Listeria Spp. Under Dynamic Conditions by Redox Potential Measurement-Based Method DOI
Oktay Haykir, Dóra Tőzsér, Csilla Mohácsi‐Farkas

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0

Influence of slope, material, and temperature on Listeria monocytogenes and Pseudomonas aeruginosa mono- and dual-species biofilms DOI
Tessa Tuytschaever, Christine Faille, Katleen Raes

et al.

Biofouling, Journal Year: 2024, Volume and Issue: 40(8), P. 467 - 482

Published: July 25, 2024

Understanding factors influencing Listeria monocytogenes biofilms aid in developing more effective elimination/prevention strategies. This study examined the effect of temperature (4 °C, 21 30 °C), materials (stainless steel 316 L with 2B and 2 R finishes, glass, polypropylene), slope (0°/horizontal or 90°/vertical) on mono- dual-species using two L. strains one Pseudomonas aeruginosa strain. All were grown 10% TSB for 24 h analyzed culture-based methods. Additionally, architecture monospecies was studied fluorescence microscopy. Overall, P. showed higher biofilm formation potential (6.2 log CFU/cm2) than (4.0 CFU/cm2). Temperature greatly influenced varied monocytogenes. The predominantly biofilms, cell counts increasing by up to CFU/cm2. Surface material had little impact formation. highlights varying effects different parameters multispecies importance surface geometry.

Language: Английский

Citations

0

Heat resistance of Listeria monocytogenes persistent cells following osmotic stress in cooked pork sausages DOI
Luca Nalbone,

Salvatore Forgia,

Graziella Ziino

et al.

International Journal of Food Science & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Summary Persistent bacteria are subset of cells capable surviving bactericidal treatments longer. They originate in response to environmental stressors and show a longer lag phase compared the rest population they part of. Impact persistent on food safety role common preservation techniques their formation still unclear. This study aims investigate whether osmotic stress induces Listeria monocytogenes resisting during cooking cycle pork sausages. Lag‐phase duration at single‐cell level strain grown 6%‐NaCl broth using image processing software was first investigated. Then, trend inactivation curve evaluated experimentally contaminated sausages cooked 65 °C for 11 min. Results showed that salt triggers characterised by increased heat resistance, posing concern especially ready‐to‐eat foods.

Language: Английский

Citations

0

Modelling growth of two Listeria monocytogenes strains, persistent and non-persistent: effect of temperature DOI Creative Commons
Ľubomí­r Valí­k,

Jana Minarovičová,

Eva Kaclı́ková

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(24), P. e40936 - e40936

Published: Dec. 1, 2024

Better growth is a phenotypic trait that can contribute to persistence of

Language: Английский

Citations

0

Listeria monocytogenes Challenge Testing of Ready-to-Eat Uncured Vacuum-Packed Pork Bars with Dried Plasma DOI Creative Commons
Paweł Pniewski, Krzysztof Anusz, Michał Tracz

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(24), P. 12055 - 12055

Published: Dec. 23, 2024

Newly developed formulas of ready-to-eat (RTE) products, despite conforming to the latest nutritional trends involving low-processed and high-protein may pose a risk promoting growth Listeria monocytogenes during shelf life. Food safety experts recommend challenge tests assess potential L. monocytogenes, which will ultimately provide microbiological evidence determine food limit. The present study evaluated whether RTE uncured vacuum-packed pork bars with dried plasma met 100 CFU/g level at end life under certain storage conditions aimed develop predictive models for in above product. were artificially inoculated mixture three strains two different inoculum densities 2 log 5 stored temperatures (2, 4, 6 °C) then subjected evaluation specific time intervals up 21 days. (Δ-value) contaminated was 0.36, 0.14, 0.91 2, °C, respectively, while inoculum, they −0.36, −0.40, 0.64 respectively. Statistically higher (p < 0.05) detected than CFU/g. results indicate that this type product must be classified as category: “Ready-to-eat foods able support other those intended infants special medical purposes” (Category 1.2. according EU Regulation 2073/2005). newly created can also describe an environment where factors, such temperature, pH, aw, change time. showed density statistically reduced values compared lower density.

Language: Английский

Citations

0