The microbiota of wooden cheese ripening boards is a rich source of antimicrobial-producing bacteria against Listeria monocytogenes DOI Creative Commons
Yuxing Chen,

Ibrahim Zúñiga Chaves,

Garret Suen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

ABSTRACT Wooden boards are essential tools in cheese ripening and there accumulating observations suggesting their antimicrobial effect against foodborne bacterial pathogens, such as Listeria monocytogenes . However, poor recovery of bacteria from wood can confound quantification pathogen burdens. To assess L. survival on wooden boards, we applied a disruptive grinding method tracked native board-associated counts controls. Our data revealed that declines clean zones but replicate areas where is suitable cheese. microbiota analysis diverse communities board surfaces, with prominent presence Brevibacterium , Brachybacterium Staphylococcus genera. We further identified seven species inhibit belonging to Bacillus Serratia phyla, well Lactococcus lactis focused safensis isolate novel biocontrol agent candidate, found it potently via secreted factors. genomic, bioinformatic, biochemical analyses indicate those factors likely peptides encoded by multiple biosynthetic gene clusters, several which unique B. have not been characterized. A sub-inhibitory concentration supernatant induces significant down-regulation prophage elements up-regulation stress response Taken together, our findings the rich source antimicrobial-producing potential applications control strategies. IMPORTANCE Despite stringent food safety measures, outbreaks remain frequent high hospitalization mortality rates. Removal processing environments extremely challenging, because this ubiquitous encodes wide array mechanisms enable thrive under harsh conditions. study used causing noticeable decline population following surface inoculation. Bacterial diverse, harbor many produce compounds example new isolate. Therefore, promising for future discovery efforts.

Language: Английский

Determination of an Effective Sanitizing Procedure for Listeria innocua in Personal Protective Equipment Used in Dairy Facilities DOI Creative Commons

Karen Nieto-Flores,

Luis Sabillón, Jayne Stratton

et al.

Journal of Food Protection, Journal Year: 2025, Volume and Issue: unknown, P. 100455 - 100455

Published: Jan. 1, 2025

The presence of Listeria monocytogenes in the dairy environment remains a food safety challenge. source microbial contamination may include employees and their personal protective equipment (PPE). This study investigated effectiveness cleaning protocols (i.e., detergents mechanical action) three chemical sanitizers commonly employed at facilities against innocua on different types gloves, aprons, boots. Coupons made PPE material were inoculated with two-strain cocktail L. suspended either Phosphate Buffered Saline (PBS) or skim milk to determine potential effect organic matter. In general, peroxyacetic acid (0.20% (v/v)) was more effective reducing counts aprons gloves compared chlorine quaternary ammonium 200 ppm. Depending type sanitizer, reductions ranged from 1.95 - 4.72 1.52 4.60 log CFU/in2 respectively. comparison, achieved 0.93 2.32 reduction boot soles, no significant differences observed among sanitizers. PVC (vinyl) lower than nitrile latex gloves. Sanitizers less boots wider deeper lugs those shallow lugs. matter significantly reduced antimicrobial efficacy all (<1 reduction). However, inclusion without action ≥3 PPE. highlights importance scrubbing as an essential step reduce

Language: Английский

Citations

1
Phage‐derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens DOI Creative Commons
Dahee Choi,

Sangryeol Ryu,

Minsuk Kong

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 3, 2025

Abstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing. Similarly, development rapid, sensitive, accurate pathogen detection tools prerequisite ensuring food safety. Phage‐derived proteins have become innovative combating these because their potent activity host specificity. Phage are relatively free from regulation compared phages per se, there no concerns about transduction harmful genes. With recent progress in next‐generation sequencing technology, analysis phage genomes has more accessible, numerous with potential biocontrol been identified. This review provides comprehensive overview protein research on safety 2006 present, pivotal period marked by certification Generally Recognized As Safe (GRAS). Emphasizing advancements, we investigated diverse applications various purposes. While highlighting successful implementation proteins, also current bottlenecks propose strategies overcome challenges. By summarizing state phage‐derived contributes deeper understanding effective detecting pathogens.

Language: Английский

Citations

1
From Traditional Typing to Genomic Precision: Whole-Genome Sequencing of Listeria monocytogenes Isolated from Refrigerated Foods in Chile DOI Creative Commons
Julio Parra‐Flores,

Beatriz Daza-Prieto,

Pamela Chavarria

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(2), P. 290 - 290

Published: Jan. 16, 2025

Ready-to-eat (RTE) foods are the most common sources of Listeria monocytogenes transmission. Whole-genome sequencing (WGS) enhances investigation foodborne outbreaks by enabling tracking pathogen and prediction genetic traits related to virulence, stress, antimicrobial resistance, which benefit food safety management. The aim this study was evaluate efficacy WGS in typing 16 L. strains isolated from refrigerated Chile, highlighting its advantages identification improvement epidemiological surveillance safety. Using cgMLST, a cluster identified comprising 2 with zero allele differences among evaluated. Ninety-four percent isolates (15/16) were serotype 1/2b, 88% them (14/16) ST5. All shared identical virulence genes adhesion (ami, iap, lapB), stress resistance (clpCEP), invasion (aut, iapcwhA, inlAB, lpeA), toxin production (hly), intracellular regulation (prfA), only 13 exhibiting bcrBC qacJ gene, confer quaternary ammonium. pCFSAN010068_01 plasmids prevalent, insertion sequences (ISLs) composite transposons (cns) detected 87.5% strains. presence various antibiotic genes, along thermal shocks disinfectants, may provide ST5 enhanced environmental hygiene treatments used studied plant.

Language: Английский

Citations

0
Antilisterial activity of Thymus vulgaris essential oil: In vitro, in situ, and in silico investigations DOI
Abdelaziz Ed‐Dra, Luca Nalbone,

Abdelaaty A. Shahat

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107557 - 107557

Published: April 1, 2025

Language: Английский

Citations

0
Exploration of the biodiversity and mining novel target genes of Listeria monocytogenes strains isolated from beef through comparative genomics analysis DOI Creative Commons
Bo Zhang,

Wenjie Sun,

Xiaoxu Wang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

L. monocytogenes is a significant foodborne pathogen. This study aims to explore the biodiversity and evolutionary characteristics of isolated from beef through pan-genome analysis, provide important reference value for its specific molecular detection. conducted an in-depth analysis virulence genes, antimicrobial resistance environmental genes 344 strains beef. Pan-genomic revealed that have open genomes, providing solid genetic basis adaptation different environments. MLST most prevalent types were ST9 CC9. A total 50 detected in these strains, with 26 such as inlA , inlB plcA plcB prfA present all strains. The four antibiotic norB lin mprF FosX indicating high fluoroquinolones, lincosamides, peptides, phosphonic acid antibiotics. 416 potential target identified pan-genomic screening, which then further filtered using hub gene selection method mining novel genes. Ultimately, 10 highly connected selected: bglF_2 tilS group_2105 group_2431 oleD ndk flgG purB pbpB fni . These play crucial role pathogenesis PCR results demonstrated excellent specificity Moreover, artificial contamination experiment, was able effectively detect samples. Therefore, holds detection

Language: Английский

Citations

0
Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment DOI Creative Commons

Nadja Pracser,

Eva M. Voglauer,

Sarah Thalguter

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 10, 2024

The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the environment, where contamination products regularly occurs. diverse repertoire stress resistance genes, potential colonize biofilms, and support co-existing microbiota have been proposed as root causes for survival L. environments. In this study, 71 sites were sampled after cleaning disinfection European frozen vegetable facility, in-house clones persisted years. innocua detected by culture-dependent method at 14 sampling sites, primarily on conveyor belts associated parts. presence determined quantification bacterial load analysis extracellular matrix components (carbohydrates, proteins, DNA) was confirmed nine (12.7%). two cases, biofilm. Furthermore, we explored resident microbial community environment biofilm-positive well co-occurrence taxa with 16S rRNA gene sequencing. Pseudomonas , Acinetobacter Exiguobacterium dominated environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned Enterobacterales ( Enterobacter Serratia unclassified Enterobacteriaceae ) Carnobacterium found be significantly higher abundant -positive samples. Several ASVs less compared -negative Acinetobacter, Janthinobacterium Brevundimonas key players more relatively biofilms. Further, composition varied between different areas surface materials.

Language: Английский

Citations

3
Challenges and opportunities for risk‐ and systems‐based control of Listeria monocytogenes transmission through food DOI Creative Commons
Alexandra Belias, Samantha Bolten, Martin Wiedmann

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(6)

Published: Nov. 1, 2024

Abstract Listeria monocytogenes contamination of ready‐to‐eat (RTE) food products and food‐associated built environments (e.g., processing facilities) represents a safety issue with major public health business risk implications. A number factors make L. control particular challenge, including (i) its frequent presence in different environments, (ii) propensity for establishing persistence (iii) ability to grow under variety stressful conditions, (iv) cause severe illness, particularly immunocompromised individuals pregnant people. Key sources RTE foods are environments. However, raw materials can also be an important source, without “kill step” fresh produce, dairy products, cold‐smoked seafood). While certain deli meats, soft cheeses, produce) have commonly been linked listeriosis outbreaks, cases, recalls, will influence the specific given represents, likelihood contamination, support growth, consumer‐related (including consumption by or individuals). Consequently, risk‐based approach presents most appropriate strategy minimize impact . challenges include development implementation systems that prevent minimizing material sources, effective root analysis procedures, growth finished product, (v) consumer education.

Language: Английский

Citations

3
Inhibitory effect of Lactococcus lactis Nagasaki-SU6 isolated from algal beach cast on Listeria monocytogenes during and after fermentation of cow- and soymilk DOI Creative Commons
Takashi Kuda,

Akitoshi Fujii,

Ayaka Nakamura

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 58, P. 103762 - 103762

Published: Feb. 22, 2024

Language: Английский

Citations

2
Precise pathogen quantification by CRISPR-Cas: a sweet but tough nut to crack DOI
Zhihao Yao,

Wanglu Li,

Kaiyu He

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Sept. 17, 2024

Pathogen detection is increasingly applied in medical diagnosis, food processing and safety, environmental monitoring. Rapid, sensitive, accurate pathogen quantification the most critical prerequisite for assessing protocols preventing risks. Among various methods evolved, those based on clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) have been developed as important strategies due to their distinct advantages of rapid target recognition, programmability, ultra-specificity, potential scalability point-of-care testing (POCT). However, arguments concerns quantitative capability CRISPR-based are ongoing. Herein, we systematically overview according principles, properties, application scenarios. Notably, review future challenges perspectives address precise by CRISPR-Cas. We hope insights presented this will benefit development methods.

Language: Английский

Citations

1
Environmental monitoring of Listeria monocytogenes contamination in dairy processing facilities combining culturing technique and molecular methods DOI Creative Commons
Sara Salza, Gianluca Piras, Rita Melillo

et al.

LWT, Journal Year: 2024, Volume and Issue: 211, P. 116870 - 116870

Published: Oct. 9, 2024

Language: Английский

Citations

1