bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 15, 2024
ABSTRACT
Wooden
boards
are
essential
tools
in
cheese
ripening
and
there
accumulating
observations
suggesting
their
antimicrobial
effect
against
foodborne
bacterial
pathogens,
such
as
Listeria
monocytogenes
.
However,
poor
recovery
of
bacteria
from
wood
can
confound
quantification
pathogen
burdens.
To
assess
L.
survival
on
wooden
boards,
we
applied
a
disruptive
grinding
method
tracked
native
board-associated
counts
controls.
Our
data
revealed
that
declines
clean
zones
but
replicate
areas
where
is
suitable
cheese.
microbiota
analysis
diverse
communities
board
surfaces,
with
prominent
presence
Brevibacterium
,
Brachybacterium
Staphylococcus
genera.
We
further
identified
seven
species
inhibit
belonging
to
Bacillus
Serratia
phyla,
well
Lactococcus
lactis
focused
safensis
isolate
novel
biocontrol
agent
candidate,
found
it
potently
via
secreted
factors.
genomic,
bioinformatic,
biochemical
analyses
indicate
those
factors
likely
peptides
encoded
by
multiple
biosynthetic
gene
clusters,
several
which
unique
B.
have
not
been
characterized.
A
sub-inhibitory
concentration
supernatant
induces
significant
down-regulation
prophage
elements
up-regulation
stress
response
Taken
together,
our
findings
the
rich
source
antimicrobial-producing
potential
applications
control
strategies.
IMPORTANCE
Despite
stringent
food
safety
measures,
outbreaks
remain
frequent
high
hospitalization
mortality
rates.
Removal
processing
environments
extremely
challenging,
because
this
ubiquitous
encodes
wide
array
mechanisms
enable
thrive
under
harsh
conditions.
study
used
causing
noticeable
decline
population
following
surface
inoculation.
Bacterial
diverse,
harbor
many
produce
compounds
example
new
isolate.
Therefore,
promising
for
future
discovery
efforts.
LWT,
Journal Year:
2024,
Volume and Issue:
198, P. 116040 - 116040
Published: April 1, 2024
Human
listeriosis
is
a
foodborne
disease
caused
by
Listeria
monocytogenes
contamination.
Most
cases
occur
due
to
consumption
of
ready-to-eat
(RTE)
foods.
Stewed
meat
in
seasoning,
type
pre-packaged
food,
popular
traditional
product
China.
We
used
whole
genome
sequencing
analyze
core
multilocus
sequence
typing
(cgMLST),
serogroups
and
(ST)
67
L.
isolates.
The
serogroup
IIb-ST5
was
predominantly
isolated
from
different
sources
linked
stewed
seasoning
Brand
A,
B,
C
with
<7
allelic
differences
cgMLST,
suggesting
that
this
kind
food
may
be
high-risk
causing
sporadic
listeriosis.
Furthermore,
isolates
batches
products
processed
the
same
processing
plant
had
<5
five
differences,
confirming
contamination
occurred
during
manufacture.
Additionally,
16
Plant
A
were
clone
(0
difference),
which
confirmed
strong
ability
survive
environment.
Thus,
increased
frequent
monitoring,
as
well
constant
technical
support
for
control
plants
necessary.
Foods,
Journal Year:
2024,
Volume and Issue:
13(9), P. 1364 - 1364
Published: April 28, 2024
Traditional
and
regional
foods
have
been
increasing
in
popularity
among
consumers
Poland
for
many
years.
The
observed
trend
of
searching
natural
authentic
taste
encourages
producers
to
craft
products
from
raw
milk,
including
Koryciński
cheeses.
aim
this
study
was
assess
the
microbiological
hazards
resulting
presence
pathogenic
bacteria
cheeses
available
retail
trade.
tests
were
carried
out
using
accredited
methods,
detection
Salmonella
spp.,
enumeration
Listeria
monocytogenes,
coagulase-positive
staphylococci,
staphylococcal
enterotoxins
food
when
number
staphylococci
sample
exceeded
limit
105
cfu/g.
research
material
consisted
45
conducted
revealed
that
spp.
not
detected
any
examined
However,
present
68.9%
samples.
In
as
15
tested
cheeses,
level
S.
aureus
contamination
above
cfu/g;
therefore,
these
samples
enterotoxins.
found
one
cheese.
four
L.
monocytogenes
102
cfu/g,
specified
Regulation
2073/2005
on
criteria
foodstuffs.
obtained
results
confirm
validity
monitoring
quality
need
increase
awareness
ensuring
proper
hygienic
conditions
production,
increased
risk
associated
with
unpasteurized
milk
products.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(4), P. 785 - 785
Published: April 12, 2024
Listeria
monocytogenes
poses
a
threat
to
both
human
and
animal
health.
This
work
describes
an
L.
outbreak
in
Portuguese
rabbit
farm,
detailing
the
isolates’
clinical
manifestations,
necropsy
findings,
phenotypic
genomic
profiles.
Clinical
signs,
exclusively
observed
does,
included
lethargy
reproductive
signs.
Post-mortem
examination
of
does
revealed
splenomegaly,
hepatomegaly
with
reticular
pattern,
pulmonary
congestion,
haemorrhagic
lesions
uterus,
thickening
uterine
wall
purulent
greyish
exudates.
Positive
samples
were
identified
fattening
maternity
units
across
different
samples,
encompassing
environmental
samples.
Core-genome
Multi
Locus
Sequence
Typing
(cgMLST)
analysis
confirmed
outbreak,
16
sequenced
isolates
(lineage
II,
CC31,
ST325)
clustering
within
≤2
allelic
difference
(AD)
threshold.
Antimicrobial
susceptibility
testing
for
five
antibiotics
that
15
out
19
resistant
sulfamethoxazole-trimethoprim
(SXT).
Concordantly,
all
SXT-resistant
found
harbour
plasmid
containing
trimethoprim-resistance
gene
(dfrD),
along
loci
linked
resistance
lincosamides
(lnuG),
macrolides
(mphB),
polyether
ionophores
(NarAB
operon).
All
carried
antibiotic
resistance-related
genes
tetM,
fosX,
lin,
norB,
lmrB,
sul,
mprF.
The
cluster
comprises
from
environment,
which
underscores
ubiquitous
presence
emphasizes
importance
biosecurity
measures.
Despite
limited
data
on
listeriosis
farming,
this
reveals
its
significant
impact
welfare
production.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 15, 2024
ABSTRACT
Wooden
boards
are
essential
tools
in
cheese
ripening
and
there
accumulating
observations
suggesting
their
antimicrobial
effect
against
foodborne
bacterial
pathogens,
such
as
Listeria
monocytogenes
.
However,
poor
recovery
of
bacteria
from
wood
can
confound
quantification
pathogen
burdens.
To
assess
L.
survival
on
wooden
boards,
we
applied
a
disruptive
grinding
method
tracked
native
board-associated
counts
controls.
Our
data
revealed
that
declines
clean
zones
but
replicate
areas
where
is
suitable
cheese.
microbiota
analysis
diverse
communities
board
surfaces,
with
prominent
presence
Brevibacterium
,
Brachybacterium
Staphylococcus
genera.
We
further
identified
seven
species
inhibit
belonging
to
Bacillus
Serratia
phyla,
well
Lactococcus
lactis
focused
safensis
isolate
novel
biocontrol
agent
candidate,
found
it
potently
via
secreted
factors.
genomic,
bioinformatic,
biochemical
analyses
indicate
those
factors
likely
peptides
encoded
by
multiple
biosynthetic
gene
clusters,
several
which
unique
B.
have
not
been
characterized.
A
sub-inhibitory
concentration
supernatant
induces
significant
down-regulation
prophage
elements
up-regulation
stress
response
Taken
together,
our
findings
the
rich
source
antimicrobial-producing
potential
applications
control
strategies.
IMPORTANCE
Despite
stringent
food
safety
measures,
outbreaks
remain
frequent
high
hospitalization
mortality
rates.
Removal
processing
environments
extremely
challenging,
because
this
ubiquitous
encodes
wide
array
mechanisms
enable
thrive
under
harsh
conditions.
study
used
causing
noticeable
decline
population
following
surface
inoculation.
Bacterial
diverse,
harbor
many
produce
compounds
example
new
isolate.
Therefore,
promising
for
future
discovery
efforts.
