Toxins,
Journal Year:
2024,
Volume and Issue:
16(12), P. 535 - 535
Published: Dec. 11, 2024
Food
poisoning
outbreaks
frequently
involve
staphylococcal
enterotoxins
(SEs).
SEs
include
33
distinct
types
and
multiple
sequence
variants
per
SE
type.
Various
mass
spectrometry
methods
have
been
reported
for
the
detection
of
using
a
conventional
bottom-up
approach.
However,
approach
cannot
differentiate
between
all
due
to
partial
coverage,
it
requires
long
trypsin
digestion
time.
While
alternative
top-down
can
theoretically
identify
any
modifications,
generally
provides
lower
sensitivity.
In
this
study,
we
optimized
conditions
incorporated
fully
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 10, 2025
Staphylococcus
aureus
(S.
aureus)
is
a
major
pathogenic
bacterium
responsible
for
bacterial
foodborne
diseases,
making
its
rapid,
specific,
and
accurate
detection
crucial.
In
this
study,
we
develop
ratiometric
biosensor
based
on
the
recombinase
polymerase
amplification-clustered
regularly
interspaced
short
palindromic
repeats/CRISPR
associated
protein
12a
(RPA-CRISPR/Cas12a)
system
Eu-metal–organic
framework
(Eu-MOF)
fluorescent
nanomaterials
high-sensitivity
of
S.
aureus,
combining
with
RPA
efficient
isothermal
amplification,
sensor
enhances
specificity
sensitivity
by
utilizing
target
activation
CRISPR/Cas12a.
The
Eu-MOF
serves
dual
function,
providing
stable
red
fluorescence
as
reference
signal
adsorbing
FAM-labeled
probes
quenching,
forming
dual-signal
that
significantly
reduces
background
interference.
This
design
enables
quantitative
over
wide
range
(7.9
×
100
to
7.9
108
CFU/mL)
low
limit
3
CFU/mL.
Overall,
these
merits
simplicity,
rapid
response,
high
sensitivity,
specificity,
offers
promising
method
accurately
evaluating
contamination
in
food
under
complex
substrate
conditions.
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(8), P. 676 - 676
Published: Aug. 9, 2024
Staphylococcus
aureus
stands
out
as
one
of
the
most
virulent
pathogens
in
genus
Staphylococcus.
This
characteristic
is
due
to
its
ability
produce
a
wide
variety
staphylococcal
enterotoxins
(SEs)
and
exotoxins,
which
turn
can
cause
food
poisoning
(SFP),
clinical
syndromes
such
skin
infections,
inflammation,
pneumonia,
sepsis,
addition
being
associated
with
development
inflammation
mammary
glands
dairy
cattle,
results
chronic
mastitis
cell
necrosis.
SEs
are
small
globular
proteins
that
combine
superantigenic
emetic
activities;
they
resistant
heat,
low
temperatures,
proteolytic
enzymes
tolerant
pH
range.
More
than
24
SE
genes
have
been
well
described
(SEA-SEE,
SEG,
SEH,
SEI,
SEJ,
SElK,
SElL,
SElM,
SElN,
SElO,
SElP,
SElQ,
SElR,
SElS,
SElT,
SElU,
SElV,
SElW,
SElX,
SElY,
SElZ),
part
different
SFP
outbreaks,
cases,
isolated
animal
strains.
In
recent
years,
new
(sel26,
sel27,
sel28,
sel31,
sel32,
sel33)
from
described,
two
variants
(seh-2p
ses-3p)
resulting
total
thirty-three
Ses,
including
nine
still
process
genetic
molecular
structure
evaluation.
encoded
by
located
mobile
elements,
plasmids,
prophages,
pathogenicity
islands,
enterotoxin
gene
cluster
(egc),
housed
genomic
island
S.
aureus.
Both
classical
SE-like
toxins
(SEls)
share
phylogenetic
relationships,
structure,
function,
sequence
homology,
characteristics
for
production
through
recombination
processes.
Due
epidemiological
importance
SEs,
their
rapid
assessment
detection
crucial
security
public
health;
this
reason,
methods
identification
developed,
liquid
chromatography
coupled
high-resolution
mass
spectrometry
(LC-HRMS),
methods,
whole-genome
sequencing;
providing
diagnosis
better
understanding
occurrence,
spread,
eradication
SEs.
review
provides
scientific
information
on
produced
aureus,
structural
characteristics,
organization,
regulatory
mechanisms,
superantigen
activity,
mechanisms
action
used
at
time
interaction
immune
system,
biocontrol
techniques
food.
Comprehensive Reviews in Food Science and Food Safety,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Jan. 1, 2025
Abstract
This
review
focuses
on
antimicrobial
packaging
for
food
safety,
critically
examining
the
activity
and
efficacy
of
cannabinoids
against
commonly
found
microorganisms
exploring
their
mechanisms.
Specifically,
considers
derived
from
industrial
hemp
plants,
which
are
characterized
by
low
levels
psychoactive
components.
It
also
outlines
viable
strategies
to
control
sustained
release
packaging,
enabling
extended
storage
enhanced
safety
products.
Research
demonstrates
that
effective
both
foodborne
bacteria
fungi,
with
action
primarily
attributed
microbial
membrane
instability.
Cannabinoids
can
be
utilized
prepare
films
edible
coatings;
however,
number
studies
in
this
area
remains
limited.
The
potential
contribute
intelligent
systems
is
discussed,
an
emphasis
regulatory
aspects
challenges
associated
incorporating
into
packaging.
Finally,
identifies
future
research
directions
address
current
limitations
advance
hemp‐based
solutions.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 25, 2025
Food
poisoning
caused
by
Staphylococcus
aureus
(S.
aureus)
and
its
enterotoxins
has
become
a
global
food
safety
issue.
Herein,
three
types
of
Staphylococcal
(SE),
including
SEC1,
SEC2,
SEC3,
were
successfully
expressed
immunized
in
mice
to
prepare
monoclonal
antibodies
(mAbs).
We
screened
pair
mAbs
16E12-9B7
from
12
strains
that
could
simultaneously
recognize
SEC3.
Furthermore,
the
genes
hybridoma
cells
9B7
16E12
extracted,
amplified,
inserted
into
expression
vectors
obtain
recombinant
(rAbs),
whose
affinities
consistent
with
those
ascites
antibodies.
The
paired
rAbs
applied
gold
immunochromatographic
strip
(GICS)
system
enable
rapid
detection
SEC3
visual
limits
(vLOD)
2,
0.5,
2
ng/mL
milk
samples.
Noticeably,
we
found
hydrogen
bonds
salt
bridges
played
significant
role
these
antigen–antibody
interactions.
key
sites
for
ASN28
SER31
complementarity
determining
region
(CDR)
SER32
variable
light
chain
(VL)
ARG100,
SER101,
TYR102
heavy
(VH).
analysis
potential
screening
mutant
