Phage‐derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 3, 2025

Abstract The emergence of antimicrobial‐resistant foodborne pathogens poses a continuous health risk and economic burden as they can easily spread through contaminated food. Therefore, the demand for new antimicrobial agents to address this problem is steadily increasing. Similarly, development rapid, sensitive, accurate pathogen detection tools prerequisite ensuring food safety. Phage‐derived proteins have become innovative combating these because their potent activity host specificity. Phage are relatively free from regulation compared phages per se, there no concerns about transduction harmful genes. With recent progress in next‐generation sequencing technology, analysis phage genomes has more accessible, numerous with potential biocontrol been identified. This review provides comprehensive overview protein research on safety 2006 present, pivotal period marked by certification Generally Recognized As Safe (GRAS). Emphasizing advancements, we investigated diverse applications various purposes. While highlighting successful implementation proteins, also current bottlenecks propose strategies overcome challenges. By summarizing state phage‐derived contributes deeper understanding effective detecting pathogens.

Language: Английский

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Benzalkonium chloride disinfectant residues stimulate biofilm formation and increase survival of Vibrio bacterial pathogens

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Feb. 7, 2024

Vibrio spp. are opportunistic human and animal pathogens found ubiquitously in marine environments. Globally, there is a predicted rise the prevalence of due to increasing ocean temperatures, which carries significant implications for public health seafood industry. Consequently, an urgent need enhanced strategies control prevent contamination, particularly aquaculture processing facilities. Presently, these industries employ various disinfectants, including benzalkonium chloride (BAC), as part their management strategies. While higher concentrations BAC may be effective against pathogens, inadequate rinsing post-disinfection could result residual surrounding environment. This study aimed investigate adaptation survival exposed varying residues. Results revealed that bacteria, when exposed, exhibited phenotypic characterized by increase biofilm biomass. Importantly, this effect was strain-specific rather than species-specific. Exposure residues induced physiological changes biofilms, leading number injured alive cells within biofilm. The exact nature “injured” bacteria remains unclear, but it postulated might heighten risk viable non-culturable (VBNC) development. These VBNC pose threat, especially since they cannot detected using standard culture-based methods commonly employed microbiological assessment industries. undetected presence recurrent contamination events subsequent disease outbreaks. provides evidence regarding role c-di-GMP signaling pathways mechanisms suggests mediated repression potential avenue further research. findings underscore misuse overuse development bacterial chain.

Language: Английский

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Genomic Insight into Vibrio Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels

Pathogens, Journal Year: 2025, Volume and Issue: 14(1), P. 52 - 52

Published: Jan. 10, 2025

Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due Vibrio contamination. This study analyzed the prevalence, density, species diversity, molecular traits of spp. in 48 fresh wild (FRMs) ready-to-eat stuffed (RTE-SMs) through genome analysis, assessing health risks. The results showed prevalence rates 12.5% FRMs 4.2% RTE-SMs, with V. alginolyticus as most common (46.7%). It was determined that seasonal distribution samples higher summer months. sizes ranged from approximately 3.9 6.1 Mb, GC contents varying between 41.9% 50.4%. A total 22 virulence factor (VF) classes up six antimicrobial resistance (AMR) genes were detected different species. presence nine biosynthetic gene clusters (BGCs), 27 prophage regions, eight CRISPR/Cas systems 15 strains provides information about their potential pathogenicity, survival strategies, adaptation habitats. Overall, this a comprehensive understanding genomic diversity isolated FRM RTE-SM samples, shedding light on toxicity mechanisms Vibrio-induced gastroenteritis.

Language: Английский

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Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported cause mortalities in shrimp, with phage therapy presenting an alternative eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore, this study aimed isolate characterize phages their applicability lysing parahaemolyticus. A novel vB_VpaS_BP15 (BP15) belonged the subfamily Queuovirinae icosahedral head measuring 69.11 ± 5.38 nm length 65.40 6.89 width, a non-contractile sheathed tail 139.81 14.79 nm. The one-step growth curve indicated latent period of 30 min burst size 120 PFUs per cell. Phage BP15 exhibited tolerance range temperatures pH values. Infection dynamic curves demonstrated that was highly effective against BCRC12959 at MOIs ranging from 0.01 10; even low multiplicity infection (MOI) 0.001, still caused retention. possessed circular double-stranded DNA 59,584 bp G + C content 46.7% lacked tRNA genes, virulence lysogeny genes. These findings highlight promising potential as agent Taiwan.

Language: Английский

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Broad-Spectrum Antimicrobial Peptides Suppress Vibrio parahaemolyticus Based on Lactobacillus paracasei A1 Fermentation

Foodborne Pathogens and Disease, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Growing evidence supports the efficacy of antimicrobial peptides against foodborne pathogens, though their spectrum and mechanism can vary depending on origin. We investigated derived from Lactobacillus paracasei A1, effects survival rate bactericidal mechanisms Vibrio parahaemolyticus, identified functional short within them. The crude extracts exhibited antibacterial properties 13 pathogenic bacteria, showing strong inhibition V. parahaemolyticus by disrupting structural integrity cell membranes. At minimum inhibitory concentrations, these significantly disrupted initial adhesion, membrane formation, existing biofilms effectively inhibiting pathogen spread, enhancing agents, reducing food safety risks. LC-MS/MS identification revealed four effective peptides, all demonstrating potent bacteriostatic parahaemolyticus. Our findings indicate that destroy bacterial structures as well stability regeneration biofilms, making them promising candidates for use additives to control pathogens.

Language: Английский

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Bioinformatics combined with machine learning unravels differences among environmental, seafood, and clinical isolates of Vibrio parahaemolyticus

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Vibrio parahaemolyticus is the leading cause of illnesses and outbreaks linked to seafood consumption across globe. Understanding how this pathogen may be adapted persist along farm-to-table supply chain has applications for addressing food safety. This study utilized machine learning develop robust models classifying genomic diversity V. that was isolated from environmental ( n = 176), 975), clinical 865) sample origins. We constructed a pangenome respective genome assemblies employed random forest algorithm predictive identify gene clusters encoding metabolism, virulence, antibiotic resistance were associated with isolate source type. Comparison genomes all seafood-clinical isolates showed high balanced accuracy (≥0.80) Area Under Receiver Operating Characteristics curve (≥0.87) these functional features. Major virulence factors including tdh , trh type III secretion system-related genes, four alpha-hemolysin genes hlyA hlyB hlyC hlyD ) identified as important differentiating in our model, underscoring need further investigation. Significant patterns AMR differing among samples revealed model conferring tetracycline, elfamycin, multidrug (phenicol antibiotic, diaminopyrimidine fluoroquinolone antibiotic) top three key variables. These findings provide crucial insights into development effective surveillance management strategies address public health threats .

Language: Английский

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Microbiota Dysbiosis in Mytilus chilensis Is Induced by Hypoxia, Leading to Molecular and Functional Consequences

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 825 - 825

Published: April 5, 2025

Bivalve microbiota play a vital role in host health, supporting nutrient processing, immunity, and disease resistance. However, the increasing hypoxia Chilean coastal waters, caused by climate change eutrophication, threatens to disrupt this microbial balance, potentially promoting pathogens impairing essential functions. Mytilus chilensis is vulnerable hypoxia-reoxygenation cycles, yet effects on its remain poorly understood. This study investigates impact of structure functional potential communities residing gills digestive glands M. chilensis. Employing full-length 16S rRNA gene sequencing, we explored hypoxia’s diversity capacity. Our results revealed significant alterations composition, with shift towards facultative anaerobes thriving low oxygen environments. Notably, there was decrease dominant bacterial taxa such as Rhodobacterales, while opportunistic Vibrio Aeromonas exhibited increased abundance. Functional analysis indicated decline critical functions associated metabolism immune support, jeopardizing health survival host. sheds light intricate interactions between host-associated environmental stressors, underlining importance managing face aquaculture practices.

Language: Английский

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Microbiome Diversity in Seafood Factories via Next-Generation Sequencing for Food Safety Management System (FSMS) Certifications in Malaysia

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1517 - 1517

Published: April 26, 2025

Next-Generation Sequencing (NGS) technology was applied to evaluate Food Safety Management System (FSMS) performance in seafood-processing factories by exploring microbiome diversity alongside traditional methods for detecting foodborne pathogens. A total of 210 environmental swabs collected from processing zones six underwent 16S rRNA amplicon sequencing. FSMS-certified exhibited significantly higher species richness, with alpha p-values 0.0036 observed ASVs, 0.0026 Faith’s PD and 0.032 Shannon. Beta analysis also revealed significant differences, 0.001 Bray–Curtis, unweighted UniFrac Jaccard. Pathogens like Listeria monocytogenes, Salmonella spp. Bacillus cereus were present “uncertified” but absent the “certified” factories. The had a proportion lactic acid bacteria (LAB) genera (70.22%) compared (29.78%). LAB included Streptococcus, Lactococcus, Lactobacillus others. NGS has demonstrated superior capability providing comprehensive detection, including unculturable microorganisms insights into microbial diversity, so it lacks limitations that come culturing. These findings highlight potential leveraging beneficial microbes bioremediation pathogen control enhance FSMS effectiveness environments.

Language: Английский

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A culture-independent approach, supervised machine learning, and the characterization of the microbial community composition of coastal areas across the Bay of Bengal and the Arabian Sea

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 10, 2024

Abstract Background Coastal areas are subject to various anthropogenic and natural influences. In this study, we investigated compared the characteristics of two coastal regions, Andhra Pradesh (AP) Goa (GA), focusing on pollution, activities, recreational impacts. We explored three main factors influencing differences between these coastlines: The Bay Bengal’s shallower depth lower salinity; upwelling phenomena due thermocline in Arabian Sea; high tides that can cause strong currents transport pollutants debris. Results microbial diversity GA was significantly higher than AP, which might be attributed temperature, soil type, vegetation cover. 16S rRNA amplicon sequencing bioinformatics analysis indicated presence diverse phyla, including candidate phyla radiation (CPR). Statistical analysis, random forest regression, supervised machine learning models classification confirm microbiome accurately. Furthermore, have identified 450 cultures heterotrophic, biotechnologically important bacteria. Some strains were as novel taxa based gene sequencing, showing promising potential for further study. Conclusion Thus, our study provides valuable insights into pollution levels AP GA. These findings contribute a better understanding impact activities climate variations biology ecosystems biodiversity.

Language: Английский

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Machine learning to predict the relationship between Vibrio spp. concentrations in seawater and oysters and prevalent environmental conditions

Food Research International, Journal Year: 2024, Volume and Issue: 188, P. 114464 - 114464

Published: May 10, 2024

Language: Английский

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