Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells DOI Creative Commons
Pedro Curto, Isaura Simões, Sean P. Riley

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2016, Volume and Issue: 6

Published: July 29, 2016

Spotted fever group (SFG) rickettsiae are recognized as important agents of human tick-borne diseases worldwide, such Mediterranean spotted (R. conorii) and Rocky Mountain rickettsii). Recent studies in several animal models have provided evidence non-endothelial parasitism by pathogenic SFG Rickettsia species, suggesting that the interaction with cells other than endothelium may play an role pathogenesis rickettsial diseases. These raise hypothesis macrophages been underappreciated. Herein, we evaluated ability two R. conorii (a pathogen) montanensis non-virulent member SFG) to proliferate THP-1 macrophage-like cells, or within non-phagocytic cell lines. Our results demonstrate was able survive both phagocytic epithelial vitro. In contrast, grow but drastically compromised undifferentiated PMA-differentiated cells. Interestingly, association assays revealed defective binding THP-1-derived macrophages; however, invasion bacteria adhere did not appear be affected. We also demonstrated which entered into were rapidly destroyed partially co-localized LAMP-2 cathepsin D, markers lysosomal compartments. present intact free cytoplasm types. findings suggest a phenotypic difference between non-pathogenic lies their respective provide explanation why certain species associated disease mammals.

Language: Английский

The Tick Microbiome: Why Non-pathogenic Microorganisms Matter in Tick Biology and Pathogen Transmission DOI Creative Commons
Sarah Bonnet, Florian Binetruy, Angélica Hernández-Jarguín

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2017, Volume and Issue: 7

Published: June 8, 2017

Ticks are among the most important vectors of pathogens affecting humans and other animals worldwide. They do not only carry however, as a diverse group commensal symbiotic microorganisms also present in ticks. Unlike pathogens, their biology effect on ticks remain largely unexplored, fact often neglected. Nonetheless, they can confer multiple detrimental, neutral, or beneficial effects to tick hosts, play various roles fitness, nutritional adaptation, development, reproduction, defense against environmental stress, immunity. Non-pathogenic may role driving transmission tick-borne (TBP), with many potential implications for both human animal health. In addition, genetic proximity some mutualistic symbionts hosted by is evident when studying phylogenies several bacterial genera. The best examples found within members Rickettsia, Francisella, Coxiella genera: while medical veterinary research these bacteria traditionally recognized highly virulent vertebrate it now clear evolutionary ecologists that (if most) Coxiella, Rickettsia actually non-pathogenic exhibiting alternative lifestyles symbionts. Consequently, represent compelling yet challenging system which study microbiomes microbial interactions, investigate composition, functional, ecological communities. Ultimately, deciphering relationships between well symbiont interactions will garner invaluable information, aid future development arthropod pest vector-borne pathogen control strategies.

Language: Английский

Citations

373
Cells within cells: Rickettsiales and the obligate intracellular bacterial lifestyle DOI
Jeanne Salje

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(6), P. 375 - 390

Published: Feb. 9, 2021

Language: Английский

Citations

121
16-membered macrolide antibiotics: a review DOI
Biljana Arsić, Jill Barber,

Ana Čikoš

et al.

International Journal of Antimicrobial Agents, Journal Year: 2017, Volume and Issue: 51(3), P. 283 - 298

Published: June 28, 2017

Language: Английский

Citations

151
Engineering of obligate intracellular bacteria: progress, challenges and paradigms DOI Open Access

Erin E. McClure,

Adela S. Oliva Chávez, Dana K. Shaw

et al.

Nature Reviews Microbiology, Journal Year: 2017, Volume and Issue: 15(9), P. 544 - 558

Published: June 19, 2017

Language: Английский

Citations

148
Molecular basis of mycobacterial survival in macrophages DOI
Jane Atesoh Awuh, Trude H. Flo

Cellular and Molecular Life Sciences, Journal Year: 2016, Volume and Issue: 74(9), P. 1625 - 1648

Published: Nov. 19, 2016

Language: Английский

Citations

130
Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote Spread DOI Creative Commons
Rebecca L. Lamason, E Bastounis, Natasha M. Kafai

et al.

Cell, Journal Year: 2016, Volume and Issue: 167(3), P. 670 - 683.e10

Published: Oct. 1, 2016

Language: Английский

Citations

126
WhollyRickettsia! Reconstructed Metabolic Profile of the Quintessential Bacterial Parasite of Eukaryotic Cells DOI Creative Commons
Timothy Driscoll, Victoria I. Verhoeve, Mark L. Guillotte

et al.

mBio, Journal Year: 2017, Volume and Issue: 8(5)

Published: Sept. 27, 2017

Reductive genome evolution has purged many metabolic pathways from obligate intracellular

Language: Английский

Citations

114
Emerging Roles of Toxin-Antitoxin Modules in Bacterial Pathogenesis DOI Creative Commons
Barbara Kędzierska, Finbarr Hayes

Molecules, Journal Year: 2016, Volume and Issue: 21(6), P. 790 - 790

Published: June 17, 2016

Toxin-antitoxin (TA) cassettes are encoded widely by bacteria. The modules typically comprise a protein toxin and or RNA antitoxin that sequesters the factor. Toxin activation in response to environmental cues other stresses promotes dampening of metabolism, most notably translation, which permits survival until conditions improve. Emerging evidence also implicates TAs bacterial pathogenicity. Bacterial persistence involves entry into transient semi-dormant state cells survive unfavorable including killing antibiotics, is significant clinical problem. TA complexes play fundamental role inducing downregulating cellular metabolism. biofilms important numerous chronic inflammatory infectious diseases cause serious therapeutic problems due their multidrug tolerance resistance host immune system actions. Multiple influence biofilm formation through network interactions with factors mediate production maintenance. Moreover, view emerging contributions virulence, potential targets for novel prophylactic approaches required urgently an era expanding antibiotic resistance. This review summarizes virulence profiles diverse range key pathogens trigger human disease.

Language: Английский

Citations

107
Pathogenesis of Rickettsial Diseases: Pathogenic and Immune Mechanisms of an Endotheliotropic Infection DOI Open Access
Abha Sahni, Rong Fang, Sangita Sahni

et al.

Annual Review of Pathology Mechanisms of Disease, Journal Year: 2018, Volume and Issue: 14(1), P. 127 - 152

Published: Aug. 27, 2018

Obligately intracytosolic rickettsiae that cycle between arthropod and vertebrate hosts cause human diseases with a spectrum of severity, primarily by targeting microvascular endothelial cells, resulting in dysfunction. Endothelial cells mononuclear phagocytes have important roles the intracellular killing upon activation effector molecules innate adaptive immunity. In overwhelming infection, immunosuppressive effects contribute to severity illness. Rickettsia-host cell interactions involve host receptors for rickettsial ligands mediate adhesion and, some instances, trigger induced phagocytosis. Rickettsiae interact actin effect both cellular entry actin-based mobility. The interaction also involves evasion defense mechanisms exploitation environment. Signal transduction events exemplify these effects. An intriguing frontier is array noncoding RNA their potential on pathogenesis transmission diseases.

Language: Английский

Citations

103
Genomic evolution and adaptation of arthropod-associated Rickettsia DOI Creative Commons
Khalid El Karkouri, Éric Ghigo, Didier Raoult

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: March 9, 2022

Abstract Rickettsia species are endosymbionts hosted by arthropods and known to cause mild fatal diseases in humans. Here, we analyse the evolution diversity of 34 using a pangenomic meta-analysis (80 genomes/41 plasmids). Phylogenomic trees showed that spp. diverged into two Spotted Fever groups, Typhus group, Canadensis group Bellii may have inherited their plasmids from an ancestral plasmid persisted some strains or been lost others. The results suggested ancestors might infected Acari and/or Insecta probably persisting inside switching hosts. Pangenomic analysis revealed genus evolved through strong interplay between genome degradation/reduction expansion leading possible distinct adaptive trajectories. mainly shared evolutionary relationships with α-proteobacteria, also γ/β/δ-proteobacteria, cytophagia, actinobacteria, cyanobacteria, chlamydiia viruses, suggesting lateral exchanges several critical genes. These processes orchestrated abundance mobile genetic elements, especially groups. In this study, provided global genomic view intracellular help our understanding diversity, adaptation fitness.

Language: Английский

Citations

51