MicroRNA novel‐miR‐90‐5p modulates larval molt development in Ostrinia furnacalis (Guenée) by targeting OfCYP18A1 DOI
Hong‐Yun Ruan, Xue Tang, J. Meng

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Abstract The hormone 20‐hydroxyecdysone (20E) plays an important role in the physiological processes of insect growth, development, and ecdysis, whereas CYP18A1, a 20E hydroxylase, participates degradation maintains its equilibrium state, which is indispensable part signaling pathway. MicroRNAs (miRNAs) are post‐transcriptional regulators gene expression mediate various biological processes. However, whether how miRNAs regulate CYP18A1 remains unclear. Herein, OfCYP18A1 was expressed different developmental stages tissues. After knockdown, survival ecdysis rates larvae as well their body weight length significantly decreased, causing to molt early but not completely, resulting death. concentration increased, that juvenile (JH) decreased. To further investigate regulatory mechanism , miRNA (novel‐miR‐90‐5p) targeting proposed. Novel‐miR‐90‐5p tissues Ostrinia furnacalis it exhibited inverse trend . overexpression O. decreased larval rates, delayed size, increased concentration, JH concentration. after novel‐miR‐90‐5p inhibition, rate 3rd‐instar did differ, delayed, change. These findings reveal involved regulation during growth thus enhancing our understanding defense strategies.

Language: Английский

Targeting the Hh and Hippo pathways by miR‐7 suppresses the development of insect wings DOI Open Access
Mingming Li, Xuan Yu, Zhihao Yao

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Abstract Wings are important organs of insects involved in flight, mating, and other behaviors, therefore prime targets for pest control. The formation insect wings is a complex process that regulated by multiple pathways. Hedgehog (Hh) pathway regulates the distribution wing veins, while Hippo modulates size. Any interventions can manipulate these pathways have potential to disrupt development could be used In this study, we find overexpression miR‐7 Drosophila results smaller with disordered veins. Mechanistically, directly both ci yki via different mature miRNAs (miR‐7‐5p miR‐7‐3p), thereby disrupting Hh Importantly, regulatory mechanism also observed another species, Helicoverpa armigera . Finally, utilizing nanocarrier delivery system, show introducing star polycation (SPc) leads defects H. conclusion, findings uncover inhibits targeting pathways, indicating its use control strategies.

Language: Английский

Citations

0
Insecticidal activity and systematic insights of carvacrol against Tribolium castaneum: Acetylcholinesterase inhibition, oxidative stress, and molecular docking" DOI

Hassan M. Eltalawy,

Huda El-Fayoumi,

Shawky M. Aboelhadid

et al.

Journal of Stored Products Research, Journal Year: 2025, Volume and Issue: 112, P. 102638 - 102638

Published: March 23, 2025

Language: Английский

Citations

0
Identification and Characterization of Three Novel Iflaviruses in the Cabbage Whitefly Aleyrodes proletella DOI Creative Commons

Zhuang-Xin Ye,

Guowei Gu,

Peng‐Peng Ren

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 335 - 335

Published: March 22, 2025

The cabbage whitefly (A. proletella) (Hemiptera: Aleyrodidae) is a major agricultural pest that primarily targets cruciferous crops, such as cabbage, broccoli, and kale, causing extensive damage through feeding honeydew. However, its associated virome has received limited research attention. In this study, we collected whiteflies in Xinjiang Agricultural University (43.80833 N, 87.56778 E, 882.3 m), systematically identified the RNA of A. proletella successfully three novel iflaviruses (Aleyrodes iflavirus 1 (APIV1), Aleyrodes 2 (APIV2) 3 (APIV3)). APIV1-3 all have 5' structural protein region 3' non-structural region. Phylogenetic sequence identity analyses suggest are members family Iflaviridae. Structural modeling using AlphaFold3 revealed conserved core variable outer loop This study provides valuable insights into diversity proletella, establishing foundation for future on virus-host interactions potential biocontrol applications sustainable agriculture.

Language: Английский

Citations

0
MicroRNA novel‐miR‐90‐5p modulates larval molt development in Ostrinia furnacalis (Guenée) by targeting OfCYP18A1 DOI
Hong‐Yun Ruan, Xue Tang, J. Meng

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Abstract The hormone 20‐hydroxyecdysone (20E) plays an important role in the physiological processes of insect growth, development, and ecdysis, whereas CYP18A1, a 20E hydroxylase, participates degradation maintains its equilibrium state, which is indispensable part signaling pathway. MicroRNAs (miRNAs) are post‐transcriptional regulators gene expression mediate various biological processes. However, whether how miRNAs regulate CYP18A1 remains unclear. Herein, OfCYP18A1 was expressed different developmental stages tissues. After knockdown, survival ecdysis rates larvae as well their body weight length significantly decreased, causing to molt early but not completely, resulting death. concentration increased, that juvenile (JH) decreased. To further investigate regulatory mechanism , miRNA (novel‐miR‐90‐5p) targeting proposed. Novel‐miR‐90‐5p tissues Ostrinia furnacalis it exhibited inverse trend . overexpression O. decreased larval rates, delayed size, increased concentration, JH concentration. after novel‐miR‐90‐5p inhibition, rate 3rd‐instar did differ, delayed, change. These findings reveal involved regulation during growth thus enhancing our understanding defense strategies.

Language: Английский

Citations

0