Metabolomic Insights into Energy Utilization Strategies of Asiatic Toads (Bufo gargarizans) During Hibernation

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 403 - 403

Published: Jan. 31, 2025

Hibernation is a crucial adaptive strategy for amphibians, facilitating survival in harsh environmental conditions by lowering metabolic rates and reducing energy use. This study employed GC-MS LC-MS metabolomics to systematically analyze the serum metabolome of Bufo gargarizans during hibernation, aiming uncover its adaptation mechanisms. A total 136 differentially expressed metabolites (DEMs) were identified, which 115 downregulated 21 upregulated, mainly involved amino acid, carbohydrate, lipid metabolism. KEGG pathway analysis showed that most pathways inhibited hibernating group, underscoring significant reduction overall activity. Notably, while acid carbohydrate metabolism significantly reduced, exhibited distinctive response. Enhanced β-oxidation fatty acids, including palmitoleic arachidonic sodium caprylate, suggests shift toward lipid-based utilization. The key like fumaric succinic TCA cycle further supports hypothesis reduced requirements. These results enhance our current understanding amphibian hibernation metabolisms provide targeted approach future mechanistic investigations.

Language: Английский

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Microbial communities are thermally more sensitive in warm-climate lizards compared with their cold-climate counterparts

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 15, 2024

Environmental temperature affects the composition, structure, and function of gut microbial communities in host animals. To elucidate role microbiota thermal adaptation, we designed a 2 species × 3 temperatures experiment, whereby acclimated adult males two agamid lizard (warm-climate Leiolepis reevesii cold-climate Phrynocephalus przewalskii ) to 20, 28, 36°C for weeks then collected their fecal small-intestinal samples analyze compare using 16S rRNA gene amplicon sequencing technology. The displayed more pronounced interspecific differences community than occurring thermally different regions. response increase or decrease differed between species, with bacterial taxa affected by acclimation L. P. przewalskii. Both warm-climate particular, could cope change adjusting relative abundance functional categories associated metabolism environmental information processing. Functional genes carbohydrate were enhanced , suggesting contribution adaptation . Taken together, our results validate hypotheses tested, which one suggests that should help lizards adapt environments they live, other be sensitive lizards.

Language: Английский

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Multi-Omics Analysis Reveals Dietary Fiber’s Impact on Growth, Slaughter Performance, and Gut Microbiome in Durco × Bamei Crossbred Pig

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1674 - 1674

Published: Aug. 14, 2024

Dietary fiber (DF) is an important nutrient component in pig’s diet that remarkably influences their growth and slaughter performance. The ability of pigs to digest DF depends on the microbial composition intestinal tract, particularly hindgut. However, studies how alters performance by shaping gut metabolites are still limited. Therefore, this study aimed investigate effects composition, functions, metabolites, ultimately altering host using Durco × Bamei crossbred supplemented with 0%, 10%, 17%, 24% broad bean silage basic diet. We found final weight, average daily gain, fat, lean meat weight significantly decreased increasing DF. Pigs lowest rate fat were observed fiber-supplemented group. Gut communities highest alpha diversity formed 17% relative abundance fiber-degrading bacteria, bile acid, succinate-producing including Prevotella sp., Bacteroides Ruminococcus Parabacteroides functional pathways, butanoate metabolism tricarboxylic acid [TCA] cycle, increased high-fiber groups. concentrations several acids groups, whereas succinate long-chain fatty increased. Our results indicate a may alter modulating metabolite pathways succinate.

Language: Английский

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Effects of host identity on the gut microbiota: A comparative study on three microtinae species

Animal Models and Experimental Medicine, Journal Year: 2024, Volume and Issue: 7(2), P. 98 - 105

Published: April 1, 2024

Abstract Background Gut microbiota exert an immense effect on host health and environmental adaptation. Furthermore, the composition structure of gut are determined by environment genetic factors. However, relative contribution factors toward shaping has been poorly understood. Methods In this study, we characterized fecal microbial communities closely related voles Neodon fuscus , Lasiopodomys brandtii L. mandarinus after caged feeding in laboratory for 6 months, through high‐throughput sequencing bioinformatics analysis. Results The results pairwise comparisons N. vs. revealed significant differences bacterial diversity domestication. While 991 same operational taxonomic units (OTUs) were shared three voles, there 362, 291, 303 species‐specific OTUs respectively. abundances Proteobacteria Prevotella which reported to be enriched high‐altitude populations, significantly higher than low‐altitude Firmicutes, produce various digestive enzymes energy metabolism, Spirochaetes, can degrade cellulose, found abundance subterranean that dwells earth surface. Conclusion Our findings showed some components still maintained dominance even when different species reared under conditions, suggesting these bacteria substantially influenced

Language: Английский

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Evolution, Diversity, and Conservation of Herpetofauna

Animals, Journal Year: 2024, Volume and Issue: 14(13), P. 2004 - 2004

Published: July 7, 2024

Amphibians and reptiles play a critical role in the evolution of Tetrapoda, showcasing significant diversity terms their genetics, species, morphology, life history traits, evolutionary functions [...]

Language: Английский

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Hepatic Metabolomic Responses to Low-Temperature Stress in the Invasive Turtle, Trachemys scripta elegans

Animals, Journal Year: 2024, Volume and Issue: 14(16), P. 2388 - 2388

Published: Aug. 17, 2024

Investigating the physiological and biochemical changes of ectothermic species before entering hibernation would contribute to understanding how they adapt low-temperature environments. Here, red-eared slider turtle (

Language: Английский

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Multi-omics analysis reveals the genetic and environmental factors in shaping the gut resistome of a keystone rodent species

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

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Stochastic Processes Derive Gut Fungi Community Assembly of Plateau Pikas (Ochotona curzoniae) along Altitudinal Gradients across Warm and Cold Seasons

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(10), P. 1032 - 1032

Published: Oct. 20, 2023

Although fungi occupy only a small proportion of the microbial community in intestinal tract mammals, they play important roles host fat accumulation, nutrition metabolism, metabolic health, and immune development. Here, we investigated dynamics assembly gut fungal communities plateau pikas inhabiting six altitudinal gradients across warm cold seasons. We found that relative abundances Podospora Sporormiella significantly decreased with season, whereas abundance Sarocladium increased. Alpha diversity increasing gradient Distance-decay analysis showed similarities were negatively correlated elevation. The co-occurrence network complexity along as total number nodes, edges, degree nodes decreased. Both null neutral model analyses stochastic or processes dominated both seasons ecological drift was main process explaining variation different pikas. Homogeneous selection played weak role structuring during season. Collectively, these results expand our understanding distribution patterns elucidate mechanisms maintain ecosystems mammals.

Language: Английский

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Effects of Hibernation on Colonic Epithelial Tissue and Gut Microbiota in Wild Chipmunks (Tamias sibiricus)

Animals, Journal Year: 2024, Volume and Issue: 14(10), P. 1498 - 1498

Published: May 17, 2024

The gut microbiota plays a crucial role in the host’s metabolic processes. Many studies have shown significant changes of mammals during hibernation to adapt external environment, but there is limited research on colonic epithelial tissue and wild chipmunks hibernation. This study analyzed diversity, composition, function chipmunk using 16S rRNA gene high-throughput sequencing technology, further conducted histological analysis colon. Histological colon showed an increase goblet cells group, which was adaptive change long-term fasting dominant microbial phyla were Bacteroidetes, Firmicutes, Proteobacteria, relative abundance them changed significantly. structural differences indicated that Helicobacter typhlonius Mucispirillum schaedleri increased significantly, while unclassified Prevotella-9, Prevotellaceae-UCG-001, Prevotellaceae-UCG-003 other species Prevotella decreased significantly at level. Alpha diversity richness microbiota. Beta revealed between group control group. PICRUSt2 functional prediction 15 pathways, such as lipid metabolism, xenobiotics biodegradation amino acid environmental adaptation, neurodegenerative diseases, enriched 12 including carbohydrate replication repair, translation, transcription, It can be seen hibernation, towards taxa are beneficial for reducing consumption, increasing fat adapting more strongly order better provide energy body ensure normal life activities

Language: Английский

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From Skin to Gut: Understanding Microbial Diversity in Rana amurensis and R. dybowskii

Current Microbiology, Journal Year: 2024, Volume and Issue: 81(11)

Published: Sept. 13, 2024

Language: Английский

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