Differences in Gene Expression in Germinated Soybean Seeds Among Four Cultivars, as Identified by Single Seed High-Throughput RNA Sequencing DOI Creative Commons
Kôsuke Nakamura, Takahiro Gondo, Shinji Chiba

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(12), P. 2287 - 2287

Published: Dec. 13, 2024

Gene expression in individual germinated seeds of four different soybean (Glycine max [L.] Merr.) cultivars, GL3494, OAC Kent, Williams 82, and Jackson, was examined using single seed high-throughput RNA sequencing (ssRNA-seq). The gene similar between two the same cultivar, but among cultivars. Notably, ssRNA-seq identified five genes that were not stably expressed having either no detectable sequence reads or less than mapped to exon regions chromosomes 82. These findings validated by reverse transcription polymerase chain reaction analysis. study’s results demonstrate is unique an from each cultivar. This uniqueness may affect composition content nutrients soybeans used as food ingredients.

Language: Английский

Legume Starch and Flour-Based Emulsion Gels as Adipose Tissue Mimetics in Plant-Based Meat Products DOI Creative Commons

Elyse S. Czapalay,

Stacie Dobson, Alejandro G. Marangoni

et al.

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100578 - 100578

Published: Feb. 1, 2025

Language: Английский

Citations

0
Differences in Gene Expression in Germinated Soybean Seeds Among Four Cultivars, as Identified by Single Seed High-Throughput RNA Sequencing DOI Creative Commons
Kôsuke Nakamura, Takahiro Gondo, Shinji Chiba

et al.

Agriculture, Journal Year: 2024, Volume and Issue: 14(12), P. 2287 - 2287

Published: Dec. 13, 2024

Gene expression in individual germinated seeds of four different soybean (Glycine max [L.] Merr.) cultivars, GL3494, OAC Kent, Williams 82, and Jackson, was examined using single seed high-throughput RNA sequencing (ssRNA-seq). The gene similar between two the same cultivar, but among cultivars. Notably, ssRNA-seq identified five genes that were not stably expressed having either no detectable sequence reads or less than mapped to exon regions chromosomes 82. These findings validated by reverse transcription polymerase chain reaction analysis. study’s results demonstrate is unique an from each cultivar. This uniqueness may affect composition content nutrients soybeans used as food ingredients.

Language: Английский

Citations

0