Identification of synthetic consortia from a set of plant‐beneficial bacteria

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(2)

Published: Jan. 30, 2024

Abstract The use of microbial inoculants in agriculture as biofertilisers and/or biopesticides is an appealing alternative to replace or reduce the practice agrochemicals. Plant microbiota studies are revealing different bacterial groups which populating plant microbiomes re‐energising probiotic bacteria (PPB) translational research sector. Some single‐microbial strain bioinoculants have proven valid (e.g., based on Trichoderma , mycorrhiza rhizobia); however, it now recommended consider multistrain consortia since plant‐beneficial effects often a result community‐level interactions microbiomes. A limiting step selection fitting combination strains order accomplish best beneficial effect upon inoculation. In this study, we used subset 23 previously identified and characterised rice‐beneficial colonisers design test series associated experiments aimed identify potential PPB able co‐colonise induce growth promotion. Bacterial were co‐inoculated vitro planta using several methods their co‐colonisation co‐persistence monitored. Results include identification two 5‐strain one 2‐strain displayed growth‐promoting features. Future practical applications microbiome must at identifying can be most effective crop amendments.

Language: Английский

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Composing a microbial symphony: synthetic communities for promoting plant growth

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Recent advances in experimental design of synthetic microbial communities for biocontrol application

BioControl, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

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Improving Plant Performance Through Microbiome Manipulation: The Potential Role of Current Bioengineering Approaches

Bacteria, Journal Year: 2025, Volume and Issue: 4(1), P. 12 - 12

Published: March 3, 2025

In the recent past, microbiome manipulation has emerged as a promising approach to improve plant growth performance by exploring deep insight of plant–microbe interactions. The exploration either present on an ectosphere or endosphere can provide far better understanding about potential application plant-associated microbes for improvement growth, protection from pathogen invasion, and tolerance environmental stresses diverse nature. this context, next-generation sequencing methods, omics approaches, synthetic biology have made significant progress in research are being frequently used explore intriguing role microorganisms. Despite successfulness conventional incorporation CRISPR/Cas9, RNA interference technology, rhizosphere engineering, other techniques appear be enhancing performance, against biotic abiotic stress factors. review presents significance microbe interaction, vital functional aspects, collaborative action, constraints, finally latest developments bioengineering approaches destined modulation with objective host challenged stressors.

Language: Английский

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Revisiting the current and emerging concepts of postharvest fresh fruit and vegetable pathology for next‐generation antifungal technologies

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(4)

Published: June 25, 2024

Abstract Fungal infections of fresh fruits and vegetables (FFVs) can lead to safety problems, including consumer poisoning by mycotoxins. Various strategies exist control fungal FFVs, but their effectiveness sustainability are limited. Recently, new concepts based on the microbiome pathobiome have emerged offer a more holistic perspective for advancing postharvest pathogen techniques. Understanding role in FFV is essential developing sustainable strategies. This review examines current emerging approaches pathology. It reviews what known about initiation development FFVs. As promising concept, offers insights into basic mechanisms microbial The underlying uncovered being used develop relevant global antifungal will also focus technologies developed target members FFVs improve limiting mycotoxin contamination. Specifically, this stresses related that modifying interaction between include use consortia, genomic technology manipulate host community genes, databases, deep learning, artificial intelligence identify markers. Other programming behavior using synthetic biology, sRNA technology, phages, quorum sensing, quenching Rapid adoption commercialization these recommended further overall

Language: Английский

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Antibiotic‐producing plant‐associated bacteria, anti‐virulence therapy and microbiome engineering: Integrated approaches in sustainable agriculture

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(10)

Published: Oct. 1, 2024

Abstract Plant health is crucial for maintaining the well‐being of humans, animals and environment. pathogens pose significant challenges to agricultural production, global food security ecosystem biodiversity. This problem exacerbated by impact climate change, which expected alter emergence evolution plant their interaction with hosts. Traditional approaches managing phytopathogens involved use chemical pesticides, but alternative strategies are needed address ongoing decline in performance as well negative on environment public health. Here, we highlight advancement effectiveness biocontrol based antimicrobial‐producing plant‐associated bacteria, anti‐virulence therapy (e.g. quorum quenching) microbiome engineering sustainable biotechnological promote foster agriculture. Notably, Enterobacterales emerging important agents a source new antimicrobials potential use. We analysed here genomes over 250 enterobacteria examine synthesize secondary metabolites. Exploration major interest search eco‐friendly alternatives reducing pesticides.

Language: Английский

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Bacillus megaterium: Evaluation of Chemical Nature of Metabolites and Their Antioxidant and Agronomics Properties

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3235 - 3235

Published: March 12, 2024

Bacillus megaterium is particularly known for its abundance in soils and plant growth promotion. To characterize the metabolites excreted by this specie, we performed successive liquid/liquid extractions from bacteria culture medium with different polarity solvents (cyclohexane, dichloromethane, ethyl acetate butanol) to separate groups. The extracts were characterized regarding their total phenolic content, amount of reducing sugar, concentration primary amines proteins, chromatographic profile HPLC-DAD-ELSD chemical identification GC-MS. Among 75 compounds which are produced bacteria, 19 identifications first time found as B. 23 described genus. containing showed interesting agronomic activity, a global inhibition seed germination rates soya, sunflower, corn ray grass, but not corn, compared alone. Our results suggest that can produce various metabolites, like butanediol, cyclic dipeptides, fatty acids, hydrocarbons, diverse effects sometimes opposite order modulate response adapt environmental effects. These findings provide new insight into bioactive properties species therapeutic uses on plants.

Language: Английский

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Development of a Pseudomonas-based biocontrol consortium with effective root colonization and extended beneficial side effects for plants under high-temperature stress

Microbiological Research, Journal Year: 2024, Volume and Issue: 285, P. 127761 - 127761

Published: May 14, 2024

The root microbiota plays a crucial role in plant performance. use of microbial consortia is considered very useful tool for studying interactions the rhizosphere different agricultural crop plants. Thus, consortium 3 compatible beneficial rhizospheric Pseudomonas strains previously isolated from avocado rhizosphere, was constructed. composed two biocontrol P. chlororaphis (PCL1601 and PCL1606), rhizobacterium alcaligenes AVO110, which are all efficient colonizers tomato These three were with each other reached stable levels both liquid media on roots. Bacterial fluorescent tagged, colonization-related traits analyzed vitro, revealing formation mixed biofilm networks without exclusion any strains. Additionally, bacterial colonization patterns observed, high survival bacteria composing shared same habitat exhibited activity against soil-borne fungal pathogens at similar to those displayed by individual As expected, because these roots, this Pseudomonas-based had more counts roots than roots; however, inoculation shown protect plants under high-temperature stress. results revealed that has side effect stress, thus improving potential performance We concluded rhizobacterial do not improve protection phytopathogenic fungi single strains; its can show an specific improvement horticultural non-host (such as tomato) when challenged temperature extending benefical consortium.

Language: Английский

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Sorghum rhizosphere bacteriome studies and generation of multistrain beneficial bacterial consortia

Microbiological Research, Journal Year: 2024, Volume and Issue: 292, P. 128036 - 128036

Published: Dec. 27, 2024

Language: Английский

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Sorghum rhizosphere bacteriome studies to pinpoint, isolate and assess plant beneficial bacteria

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Background In the intricate relationship between plants and microorganisms, plant growth-promoting bacteria (PGPB) play a vital role in rhizosphere. This study focuses on designing synthetic bacterial consortia using key strains mapped isolated from sorghum rhizosphere microbiome. Results A large set of samples bacteriome Sorghum bicolor was analyzed across various genotypes geographical locations. We assessed taxonomic composition structure root-associated community 16S rRNA gene amplicon profiling, identifying taxa core-bacterial components. 321 was then isolated, three multi-strain were designed by combining culturable unculturable microbiome-derived information. Subsequently, co-existence plant-growth promoting ability tested both in vitro planta. growth-chamber in-field experiments demonstrated that Consortia 3 promoted growth conditions while 1 2 performed better field-plot experiments. Despite these differences, profiling confirmed stable colonization inoculated without significant alterations to overall community. Conclusions aims at translating microbiome knowledge into applications testing microbiome-based growth.

Language: Английский

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