Harmful Algae, Journal Year: 2024, Volume and Issue: 139, P. 102725 - 102725
Published: Sept. 12, 2024
Language: Английский
Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(8), P. 11406 - 11427
Published: Jan. 6, 2024
Language: Английский
Citations
3
Environmental DNA, Journal Year: 2023, Volume and Issue: 5(5), P. 945 - 959
Published: Jan. 22, 2023
Abstract Antarctic krill ( Euphausia superba ) is a keystone species in the Southern Ocean ecosystem, and monitoring its distribution abundance crucial for sustainable management of expanding fisheries targeting species. Environmental DNA (eDNA)‐based could complement conventional surveys, but applicability limited by lack knowledge on eDNA persistence decay Ocean. We aimed to develop method that can not only quantify eDNA, also estimate relative time since this was shed (“recent” vs “older”). Three species‐specific qPCR markers mitochondrial 16S region were developed, characteristics these determined through tank experiments. Krill partially degraded all samples, even when present. Marker concentrations decreased exponentially at similar rates after removal, with initial abundances maintained across three markers. Over time, concentration longest marker faster, changing markers, allowing discrimination more recent samples from older samples. employed new collected 4800 km transect, estimated age based adding temporal aspect quantitative survey. compared Euphausiid‐specific metabarcoding assess sensitivity detecting eDNA. While methods should be evaluated against existing non‐molecular survey methods, they add an important novel, dynamic layer information future surveys. Our determine where present light how may using certain habitats, our understanding species’ life cycle contributing accurate estimates.
Language: Английский
Citations
8
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 169550 - 169550
Published: Dec. 21, 2023
Language: Английский
Citations
8
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175657 - 175657
Published: Aug. 22, 2024
The Southern Ocean surrounding Antarctica harbours some of the most pristine marine environments remaining, but is increasingly vulnerable to anthropogenic pressures, climate change, and invasion by non-native species. Monitoring biotic responses cumulative impacts requires temporal spatial baselines ongoing monitoring - traditionally, this has been obtained continuous plankton recorder (CPR) surveys. Here, we conduct one longest environmental DNA (eDNA) transects yet, spanning over 3000 nautical miles from Hobart (Australia) Davis Station (Antarctica). We evaluate eDNA sampling strategies for long-term open ocean biomonitoring comparing two water volume filter pore size combinations: large (12 l with 20 μm) small (2 0.45 μm). Employing a broad COI metabarcoding assay, found sample/pore combination was better suited monitoring, detecting more target rare or low abundance Comparisons four simultaneously conducted CPR revealed that detections were diverse than CPR, 7 (4 unique) 4 (1 phyla respectively. While both methods effectively delineated biodiversity patterns across Ocean, enables surveys in presence sea-ice where cannot be conducted. Accordingly, 16 species concern detected along transect using eDNA, notably Antarctic region (south 60°S). These largely attributed hull biofouling, recognized pathway introductions into Antarctica. Given vulnerability potential warming work underscores importance continued biosecurity vigilance. advocate integrating emphasising urgency its implementation. anticipate interweaving biophysical data will generate nuanced picture ecosystems, significant implications conservation preservation ecosystems.
Language: Английский
Citations
2
Harmful Algae, Journal Year: 2024, Volume and Issue: 139, P. 102725 - 102725
Published: Sept. 12, 2024
Language: Английский
Citations
2