Phenological mismatches mitigate the ecological impact of a biological invader on amphibian communities

Ecological Applications, Journal Year: 2024, Volume and Issue: 34(6)

Published: Aug. 8, 2024

Horizon scans have emerged as a valuable tool to anticipate the incoming invasive alien species (IAS) by judging on their potential impacts. However, little research has been conducted quantifying actual impacts and assessing causes of species-specific vulnerabilities particular IAS due persistent methodological challenges. The underlying interspecific mechanisms driving therefore remain poorly understood, even though they can substantially improve accuracy risk assessments. Given that interactions ecological are often shaped phenological synchrony, we tested hypothesis temporal mismatches in breeding phenology between native mitigate Focusing American bullfrog (Lithobates catesbeianus), combined an environmental DNA (eDNA) quantitative barcoding metabarcoding survey Belgium with global meta-analysis, integrated citizen-science data phenology. We examined whether presence amphibian was negatively related or abundance bullfrogs this relationship affected mismatches. field study revealed significant negative effect increasing eDNA concentrations richness community structure. These observations were bullfrogs, late spring- summer-breeding being strongly affected, while winter-breeding remained unaffected. This trend confirmed meta-analysis. A observed mismatch impact bullfrogs. Specifically, differing 6 weeks less from more likely be absent than whose differed Taken together, present novel method based combination aqueous quantify biological invaders at level. show strong predictor invasion regardless context. Therefore, advocate for integration alignment IAS's phenologies into frameworks.

Language: Английский

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Environmental DNA metabarcoding reflects spatiotemporal fish community shifts in the Scheldt estuary

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 934, P. 173242 - 173242

Published: May 17, 2024

Estuarine ecosystems face increasing anthropogenic pressures, necessitating effective monitoring methods to mitigate their impacts on the biodiversity they harbour. The use of environmental DNA (eDNA) based detection is increasingly recognized as a promising tool complement other, potentially invasive techniques. Integrating such eDNA analyses into frameworks for large still challenging and requires deeper understanding scale resolution at which patterns may offer insights in species presence community composition space time. Scheldt estuary, characterized by its diverse habitats complex currents, one largest Western European tidal river systems. Until now, it remains obtain accurate information fish communities living migrating through this ecosystem, consequently confining our knowledge specific locations. To explore potential monitoring, we simultaneously combine stow net fishing with metabarcoding, assess spatiotemporal shifts estuary's communities. In total, detected 71 estuary using partly overlapping historic data gathered different study locations contrast only 42 during same survey period. Community compositions found both varied among sampling locations, driven clear correlation salinity gradient. Limited effects depth tide were observed metabarcoding data, allowing significant reduction effort future campaigns system. Our results further demonstrate that seasonal occurrence can be metabarcoding. Combining enhances vital waterway's populations, higher more efficient strategy.

Language: Английский

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Validation of Environmental DNA for Estimating Proportional and Absolute Biomass

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(5)

Published: Sept. 1, 2024

ABSTRACT Environmental DNA (eDNA) has significant potential to improve the efficiency of biological sampling and detect species that pose challenges for traditional methods. However, a key obstacle in utilizing eDNA data ecosystem management is uncertainty surrounding ability estimate abundance or biomass multiple simultaneously. In this study, we use experimental trials with known biomasses explore feasibility (1) estimating proportions from metabarcoding (2) absolute concentrations by scaling single obtained qPCR. The focal study were three gadid fishes are components marine ecosystems Alaska vary their distribution habitat use: Walleye pollock ( Gadus chalcogrammus ), Pacific cod macrocephalus Arctic Boreogadus saida ). After designing gadid‐specific primers accounting PCR biases data, found corrected read closely approximated true species. Furthermore, strong positive relationships between concentration using quantitative combined estimates derived These findings suggest it possible accurately quantify compositions metrics gadids real‐world scenarios. work provides framework developing analytical approaches can be applied other utility eDNA.

Language: Английский

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Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314210 - e0314210

Published: Jan. 24, 2025

Monitoring biodiversity on a large scale, such as in hydropower reservoirs, poses scientific challenges. Conventional methods passive fishing gear are prone to various biases, while the utilization of environmental DNA (eDNA) metabarcoding has been restricted. Most eDNA studies have primarily focused replicating results from traditional methods, which themselves limitations regarding representativeness and bias. In our study, we employed with three markers (12SrRNA, COI, 16SrRNA) evaluate an 800 km 2 reservoir. We utilized hydrodynamic modeling determine water flow velocity renewal ratio throughout study area. Additionally, conducted statistical comparisons—rarefaction curves multivariate methods—among samples alternative approach assess representation. The identified taxa previously documented reservoir by monitoring well revealed 29 –nine fishes 20 non-fish—previously unreported species. These highlight robustness technique. Our findings also indicated that randomly sampling 30% original number samples, could effectively capture same biodiversity. This enabled us comprehend reservoir’s profile propose straightforward, cost-effective protocol for future based eDNA.

Language: Английский

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Hydrological Connectivity Enhances Fish Biodiversity in Amazonian Mining Ponds: Insights from eDNA and Traditional Sampling

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract The expansion of artisanal and small-scale gold mining (ASGM) in the Madre de Dios region Peruvian Amazon has transformed primary forests into a novel wetland complex thousands abandoned ponds. Despite their ecological relevance, post-mining recovery these systems remains understudied, particularly regarding fish biodiversity recolonization. In this study, we evaluate community richness composition ponds different dimensions, years post abandonment, degree pulse flood connectivity using traditional collection-based methods environmental DNA (eDNA) with 12S COI markers. We compared two inventory contrasted results from ASGM waterbodies those obtained nearby pristine oxbow lakes. Overall, registered more at all sites eDNA vs methods, especially marker. identified 14 13 unique genera eDNA, respectively, 40 detected by both approaches, evidencing complementarity. Notably, found that flooding was main predictor species among (p-value < 0.05). 11 to 22, 23 71, 56 morphospecies non-flooded ponds, flooded lakes, respectively. Furthermore, most influenced were similar Our findings highlight role hydrological within mining-impacted wetlands. Future restoration efforts should enhance aquatic accelerate environments.

Language: Английский

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What is eDNA method standardisation and why do we need it?

Metabarcoding and Metagenomics, Journal Year: 2025, Volume and Issue: 9

Published: Feb. 13, 2025

The rapid advancement of environmental DNA (eDNA) science in the past two decades has inspired a concomitant growth development eDNA sampling and analytical methods. However, these methods are often developed by individual laboratories or institutions, which can isolate protocols within programmes, agencies regions prevent beneficial exchange data ideas. Recent efforts to advance national international coordination have resulted groundswell standardisation efforts, but there is still considerable confusion around role formal standards for regulatory research applications. With this commentary, we hope provide clarity on terminology used discussions, including differences between best practice guidelines. Additionally, discuss how method choice may be informed management scenarios review examples being inform action. community now an opportunity develop roadmap help close gaps, adoption accelerate our ability monitor biological life at scales current challenges demand.

Language: Английский

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Environmental DNA metabarcoding: Current applications and future prospects for freshwater fish monitoring

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 376, P. 124531 - 124531

Published: Feb. 16, 2025

Language: Английский

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Comparing the Fate of eDNA by Particle Sizes and Molecule Lengths in Recirculating Streams

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(2)

Published: March 1, 2025

ABSTRACT The detection of environmental DNA (eDNA) has revolutionized aquatic species monitoring, yet interpreting eDNA data remains challenging due to gaps in our understanding ecology (i.e., origin, state, transport, and fate) variability how methods are applied across the literature. A crucial aspect complexity is that a heterogeneous mix components vary size other properties, thereby influencing interactions with environment diverse ways. In this study, we explore interplay between three particle sizes (the physical dimension eDNA‐containing particles) two molecule lengths (DNA base pairs) flowing water systems. Specifically, elucidated mechanisms governing removal different using set 24 recirculating mesocosms where varied light substrate conditions. Consistent previous observations, findings revealed substantial differences small large particles. biofilm‐colonized substrate, found higher rates for smaller particles, but larger particles were removed more quickly presence any substrate. Importantly, also biofilm removes longer molecules faster, shedding on probable mechanism underlying longstanding association biofilm. Despite colonization faster molecules, analyzed (86 387 exhibited somewhat consistent behavior. combination, observations highlight an important predictor fate, fate shows few varying lengths. Furthermore, work suggests conclusions regarding from studies utilizing short markers applicable metabarcoding applications, which typically use marker

Language: Английский

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The Danube River

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 19 - 37

Published: Jan. 1, 2025

Language: Английский

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Vertical distribution of free and multi-fraction environmental DNA in a controlled aquatic system: insights into dispersal dynamics and sampling implications

Hydrobiologia, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

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