Genetic sex determination in three closely related hydrothermal vent gastropods, including one species with intersex individuals DOI

Jade Castel,

Florence Pradillon, Valérie Cueff‐Gauchard

et al.

Journal of Evolutionary Biology, Journal Year: 2024, Volume and Issue: 37(7), P. 779 - 794

Published: May 3, 2024

Abstract Molluscs have undergone many transitions between separate sexes and hermaphroditism, which is of interest in studying the evolution sex determination differentiation. Here, we combined multi-locus genotypes obtained from restriction site-associated DNA (RAD) sequencing with anatomical observations gonads three deep-sea hydrothermal vent gastropods genus Alviniconcha living southwest Pacific. We found that all species (Alviniconcha boucheti, strummeri, kojimai) share same male-heterogametic XY sex-determination system but XX A. kojimai individuals are invaded by a variable proportion male reproductive tissue. The identification Y-specific RAD loci (found only boucheti) phylogenetic analysis sex-linked shared suggested X-Y recombination has evolved differently within each species. This situation showing variation gonadal development around common provides new insights into mode poorly known opens up an opportunity to study suppression on chromosomes its association mixed or transitory sexual systems.

Language: Английский

Molecular Determination of Sex from Down and Feather in Wild and Reared Monomorphic and Dimorphic Birds at Juvenile Age DOI Creative Commons
Antonio Ciro Guaricci, Mario Cinone, Salvatore Desantis

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(6), P. 892 - 892

Published: March 20, 2025

The inability to distinguish males from females in young birds is a major obstacle for pair formation reintroduction–restocking programs and commercial–amateur breeding. Several techniques are employed address this problem, but not all of them suitable juvenile subjects. Among the various tests applied sex determination, polymerase chain reaction (PCR) one genetic tools that seems be most effective (rapid, invasive cheaper). In study, DNA was extracted down feathers make procedure less stressful nestlings. amplified by PCR, amplicon subjected restriction endonucleases when gender clearly identified PCR alone. One hundred fifty-three avian species were sexed using procedure, including 27 first time. nestlings juveniles tested, correctly identified; fact, pairs reached sexual maturity during study gave offspring.

Language: Английский

Citations

0
Genetic sex determination in three closely related hydrothermal vent gastropods, including one species with intersex individuals DOI

Jade Castel,

Florence Pradillon, Valérie Cueff‐Gauchard

et al.

Journal of Evolutionary Biology, Journal Year: 2024, Volume and Issue: 37(7), P. 779 - 794

Published: May 3, 2024

Abstract Molluscs have undergone many transitions between separate sexes and hermaphroditism, which is of interest in studying the evolution sex determination differentiation. Here, we combined multi-locus genotypes obtained from restriction site-associated DNA (RAD) sequencing with anatomical observations gonads three deep-sea hydrothermal vent gastropods genus Alviniconcha living southwest Pacific. We found that all species (Alviniconcha boucheti, strummeri, kojimai) share same male-heterogametic XY sex-determination system but XX A. kojimai individuals are invaded by a variable proportion male reproductive tissue. The identification Y-specific RAD loci (found only boucheti) phylogenetic analysis sex-linked shared suggested X-Y recombination has evolved differently within each species. This situation showing variation gonadal development around common provides new insights into mode poorly known opens up an opportunity to study suppression on chromosomes its association mixed or transitory sexual systems.

Language: Английский

Citations

2