The quantity of deposited environmental DNA in plant–arthropod interactions depends on taxon, abundance and interaction time DOI Creative Commons
Sarah Friedrich, Mattia De Vivo, Christian Ulrich Baden

et al.

Ecological Entomology, Journal Year: 2024, Volume and Issue: 49(6), P. 989 - 993

Published: Aug. 13, 2024

Abstract Environmental DNA (eDNA) metabarcoding holds great promise as a simple and efficient tool to detect plant–insect interactions. However, the influence of both abiotic biotic factors on eDNA deposition in these interactions is not well understood. It especially remains be tested how much abundances reflect frequency or intensity between insects plants. We experimentally analysed quantitative from three insect species (the southern green stink bug Nezara viridula, mustard beetle Phaedon cochleariae fall armyworm Spodoptera frugiperda ) leaves two host plants varying attractiveness: savoy cabbage ( Brassica oleracea var. sabauda , preferred host) tomato Solanum lycopersicum less host). for effects taxonomy, plant, exposure time interacting plant material. Our data show clear ecological signal deposition. Insect abundance interaction are reflected amount deposited eDNA. Moreover, significantly more was cabbage, plant. Besides drivers, very strong taxonomic bias different taxa observed. detection strongly influenced by targeted taxa, highlighting utility specifically trophic arthropods. biases preclude comparative analyses at community level.

Language: Английский

Optimizing eDNA Metabarcoding Techniques for Assessing Arthropod Communities in Tree‐Related Microhabitats DOI Creative Commons
Mohammad Jamil Shuvo, Tasmina Tabassum, Gernot Segelbacher

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

ABSTRACT Understanding the diversity and ecological roles of arthropods within tree‐related microhabitats (TreMs) is crucial for forest ecosystem conservation management. In our study, we aimed to identify most effective environmental DNA (eDNA) metabarcoding approach capturing ecologically important arthropod species primarily inhabiting near‐ground‐level TreMs. We evaluated use COI 16S primers eDNA compared direct indirect sampling methods, including lying deadwood sediment (LDS), standing (SDS), soil (SS), tree surface roller (TSRS). Our results indicated significant biases challenges, particularly in primer selection, with outperforming taxonomic resolution taxa. TSRS method effectively captured 408 OTUs at level, highest number associated TreMs other approaches. Direct from sediments revealed a higher abundance fungi than arthropods, impacting estimates. also observed habitat‐specific preferences among certain methods distinct findings underscore importance carefully selecting validating studies provide insights into complexity communities Optimized will advance monitoring techniques ecosystems inform efforts preserve

Language: Английский

Citations

0
Steppes and prairies DOI
Sara Gamboa, Manuel Hérnandez Fernández

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 200

Published: Jan. 1, 2025

Citations

0
Environmental DNA metabarcoding reliably recovers arthropod interactions which are frequently observed by video recordings of flowers DOI Creative Commons
Manuel Stothut,

Damaris Kühne,

Vanessa Ströbele

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(3)

Published: May 1, 2024

Abstract Environmental DNA (eDNA) metabarcoding promises to be a cost‐ and time‐efficient monitoring tool detect interactions of arthropods with plants. However, observation‐based verification the eDNA‐derived data is still required confirm reliability those detections, i.e., verify whether have previously interacted plant. Here, we conducted comparative analysis performance eDNA video camera observations arthropod communities associated sunflowers ( Helianthus annuus , L.). We compared taxonomic composition, interaction type, diversity by testing for an effect time occupancy on successful taxon recovery eDNA. also tested if prewashing flowers successfully removed deposition from before recording, thus enabling reset community standardized monitoring. find that recovered distinct communities, about quarter families overlapping. overlapping taxa comprised ~90% observed camera. Interestingly, more unique than cameras, but approximately two‐thirds were rare species. The biased toward plant sap‐suckers, showing such species may deposit than, example, transient pollinators. flower heads did not suffice remove all traces, suggesting plants temporally stable thought. Our work highlights great potential as plant‐arthropod interactions, particularly specialized frequently interacting taxa.

Language: Английский

Citations

3
Biomonitoring of biocontrol across the full annual cycle in temperate climates: Post‐harvest, winter and early‐season interaction data and methodological considerations for its collection DOI Creative Commons
Jordan P. Cuff, Domagoj Gajski, Radek Michalko

et al.

Agricultural and Forest Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: June 5, 2024

Abstract Conservation biocontrol, the regulation of crop pests by naturally occurring biocontrol agents (e.g., predators and parasitoids), is predominantly monitored throughout periods primary growth when exert most observable impact on yields. Pest‐focused agricultural biomonitoring often overlooks post‐harvest, winter even early‐season despite significant predator–pest interactions during these that profoundly affect pest abundance and, consequently, Rapid advances in biomonitoring, particularly detection underpin provide an opportunity to reconsider how we monitor interactions. Advances must transcend methodological innovation encompass conceptual changes monitoring systems. Here, assess existing evidence supporting importance beyond for are likely evolve periods, subsequently influencing population dynamics period. We advocate a greater concerted effort establish continuous interactions, temperate climates. To facilitate this, also summarise approaches can make it possible explore extending sampling across full annual cycle might practicalities outcomes approaches. Year‐round both crops adjacent semi‐natural habitats, will previously intractable understanding dynamics, offering potential enhance our ability optimise manipulate This would manifest reduced yield losses, infestation rates disease transmission, with concomitant long‐term financial, environmental land‐use benefits.

Language: Английский

Citations

2
Recovering plant-associated arthropod communities by eDNA metabarcoding historical herbarium specimens DOI Creative Commons
Manuel Stothut, Lisa Mahla,

Lennart Backes

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(18), P. 4318 - 4324.e6

Published: Aug. 27, 2024

Natural history collections are a priceless resource for understanding patterns and processes of biodiversity change in the Anthropocene.

Language: Английский

Citations

0
Harnessing Environmental DNA (eDNA) to Explore Frugivorous Interactions: A Case Study in Papaya (Carica papaya) and Pineapple (Ananas comosus) DOI Creative Commons
Pritam Banerjee, Jyoti Prakash Maity, Nalonda Chatterjee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Plant-animal interactions (PAIs) are critical in ecosystem function, mediating energy flow and species interactions. Traditional methods of tracking PAIs, such as morphological identification camera trapping, limited speed scalability, posing challenges for comprehensive biodiversity monitoring. Recently, environmental DNA (eDNA) metabarcoding has emerged a promising technique detecting non-destructively. This pilot study explores the application eDNA to investigate involving partially consumed intact fruits Carica papaya Ananas comosus . were performed from 36 6 fruit samples. Metabarcoding mitochondrial COI gene fragments revealed diverse range taxa, with Arthropoda, particularly insects, being most abundant. Results indicated significant differences taxonomic composition between pineapple samples, where both hold some unique well shared taxa. Furthermore, diversity also differed fruits, suggesting that serve rich sources, capturing various frugivores decomposers. Signal organisms detected through damaged allowed us capture wide array revealing insights into ecological relationships. The ASVs associated each type suggest certain taxa may showing preferences based on characteristics sugar content, texture, or chemical profile. Present work highlighted importance unraveling fruit-associated plant-animal method needs expertise, less labors, fast effective, which can be implemented monitoring economical

Language: Английский

Citations

0
Monitoring the Land and Sea: Enhancing Efficiency Through CRISPR-Cas Driven Depletion and Enrichment of Environmental DNA DOI
Anya Kardailsky, Benjamín Durán‐Vinet, Georgia Nester

et al.

The CRISPR Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Characterizing biodiversity using environmental DNA (eDNA) represents a paradigm shift in our capacity for biomonitoring complex environments, both aquatic and terrestrial. However, eDNA is limited by biases toward certain species the low taxonomic resolution of current metabarcoding approaches. Shotgun metagenomics enables collection whole ecosystem data sequencing all molecules present, allowing characterization identification. Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas)-based methods have potential to improve efficiency metagenomic low-abundant target organisms simplify analysis enrichment or nontarget depletion before sequencing. Implementation CRISPR-Cas has been due lack interest support past. This perspective synthesizes approaches study underrepresented taxa advocate further application optimization CRISPR-Cas, holding promise biomonitoring.

Language: Английский

Citations

0
The quantity of deposited environmental DNA in plant–arthropod interactions depends on taxon, abundance and interaction time DOI Creative Commons
Sarah Friedrich, Mattia De Vivo, Christian Ulrich Baden

et al.

Ecological Entomology, Journal Year: 2024, Volume and Issue: 49(6), P. 989 - 993

Published: Aug. 13, 2024

Abstract Environmental DNA (eDNA) metabarcoding holds great promise as a simple and efficient tool to detect plant–insect interactions. However, the influence of both abiotic biotic factors on eDNA deposition in these interactions is not well understood. It especially remains be tested how much abundances reflect frequency or intensity between insects plants. We experimentally analysed quantitative from three insect species (the southern green stink bug Nezara viridula, mustard beetle Phaedon cochleariae fall armyworm Spodoptera frugiperda ) leaves two host plants varying attractiveness: savoy cabbage ( Brassica oleracea var. sabauda , preferred host) tomato Solanum lycopersicum less host). for effects taxonomy, plant, exposure time interacting plant material. Our data show clear ecological signal deposition. Insect abundance interaction are reflected amount deposited eDNA. Moreover, significantly more was cabbage, plant. Besides drivers, very strong taxonomic bias different taxa observed. detection strongly influenced by targeted taxa, highlighting utility specifically trophic arthropods. biases preclude comparative analyses at community level.

Language: Английский

Citations

0