Integrating taxonomic, genetic and ecological data to explore the species richness of wild bees (Hymenoptera, Apoidea, Anthophila) of the Culuccia Peninsula (NE Sardinia, Italy) DOI Creative Commons
Matteo Annessi, Alessandra Riccieri, Marilena Marconi

et al.

Journal of Hymenoptera Research, Journal Year: 2025, Volume and Issue: 98, P. 117 - 145

Published: Feb. 10, 2025

Wild bees are essential pollinators of both native and cultivated plants, but their populations declining worldwide. Conservation efforts hindered by insufficient data, especially in the Mediterranean basin, which hosts some most diverse pollinator communities world. Particularly Sardinia, second largest island Mediterranean, information on bee fauna is still limited. The aim this work was to provide first checklist ApoideaAnthophila from an unexplored peninsula north-eastern Sardinia (Italy), combining traditional (morphologically-based) taxonomy DNA barcoding. In addition, records flower visits provided shown a visitor network enrich scarce data associations between wild plants Region. Bees were sampled April October 2022–2023 with two Malaise traps nets. extracted amplify sequences mitochondrial gene Cyotochrome oxydase I, then compared those BOLD using identification tool constructing neighbor-joining phylogenetic trees. Seventy-six different species belonging 29 genera six families collected identified. A total 212 COI obtained for 61 species, many had not yet been sequenced Italian populations. Five taxa Sardo-Corsican endemics, whereas newly recorded Sardinia. Finally, we highlight potential taxonomic issues new visit records, emphasizing need further research better understand ecology group insects toward conservation.

Language: Английский

Landscape influence on pollinator population genetic connectivity DOI Creative Commons
Anna Schleimer, Alain C. Frantz

Insect Conservation and Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Abstract Insect pollinators face numerous threats, including habitat loss and population fragmentation. The effects of human‐altered landscapes on connectivity need to be better understood inform effective mitigation measures. We examined the literature landscape heterogeneity genetic in two key pollinator groups: bees (Hymenoptera: Anthophila) hoverflies (Diptera: Syrphidae). identified 113 studies from 59 countries, covering 96 bee 21 hoverfly species. However, biased taxonomic geographical coverage limited broad conclusions regarding species susceptibility isolation. Notably, remain significantly understudied, hampering comprehensive assessments patterns. While some demonstrated differentiation across tens kilometres, others maintained entire continents. Various features, water bodies mountain ranges, often acted as barriers gene flow, while impacts deforestation, agriculture, urbanisation were mixed. Biological traits like body size, resource specialisation, sizes found influence contrasting results precluded conclusive findings. Future research should include evaluations time lags statistical power determine appropriateness selected tools for testing hypotheses recent pollinators. Owing increased interest corridors, encompassing a wider range species, habitats, improved study designs, is needed provide an evidence‐based framework conservation

Language: Английский

Citations

1
Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France DOI Creative Commons
Anaïs Marquisseau, Kamila Canale‐Tabet, Emmanuelle Labarthe

et al.

Biodiversity Data Journal, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 7, 2025

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on resolution sequences used as barcodes reliability reference databases. For wild bees, barcode coverage is consistently growing in volume, but some incorrect species annotations need be cared for. The COI (Cytochrome Oxydase subunit 1) gene, most barcoding/metabarcoding arthropods, suffers from primer bias difficulties covering all bee using classical Folmer primers. We present here a curated database 250 bp mini-barcode region 16S rRNA suitable low-cost bees applications, such eDNA analysis or sequencing ancient degraded DNA. Sequenced specimens were captured Occitania (south-west France) morphologically identified by entomologists, with total 530 individuals 171 19 genera. A customised workflow including distance-tree inferences second round entomologist observations, when necessary, was validation 348 mini-barcodes 148 species. Amongst them, 93 did not have any available before our contribution. This high-quality library data freely scientific community, aim facilitating future large-scale characterisation communities context pollinators' decline.

Language: Английский

Citations

0
Combining new technology with classic taxonomy to overcome hurdles to discovering dark taxa DOI
Jared Bernard

Systematics and Biodiversity, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 31, 2025

With numerous perils threatening biodiversity, we must remember that most of the basic units biodiversity—species—remain unknown and therefore difficult to assess. Hordes new species continue be discovered described every year. As each requires extensive work, completing description Earth's biota could require millennia, leaving many wanting automate process via genetic barcoding artificial intelligence. Over time, lesser-known groups species, referred as 'dark taxa', will occupy an increasing proportion awaiting description. dark taxa have few barcodes or images for matching algorithms, however, I propose integrating traditional taxonomy into automated workflows by linking data verified specimens using classic taxonomic keys decision trees identifying images. The roles intelligence would thus limited until can build databases specimens. This strategy vital their scientific names so signify undiscovered which is lacking in current methods.

Language: Английский

Citations

0
Integrating taxonomic, genetic and ecological data to explore the species richness of wild bees (Hymenoptera, Apoidea, Anthophila) of the Culuccia Peninsula (NE Sardinia, Italy) DOI Creative Commons
Matteo Annessi, Alessandra Riccieri, Marilena Marconi

et al.

Journal of Hymenoptera Research, Journal Year: 2025, Volume and Issue: 98, P. 117 - 145

Published: Feb. 10, 2025

Wild bees are essential pollinators of both native and cultivated plants, but their populations declining worldwide. Conservation efforts hindered by insufficient data, especially in the Mediterranean basin, which hosts some most diverse pollinator communities world. Particularly Sardinia, second largest island Mediterranean, information on bee fauna is still limited. The aim this work was to provide first checklist ApoideaAnthophila from an unexplored peninsula north-eastern Sardinia (Italy), combining traditional (morphologically-based) taxonomy DNA barcoding. In addition, records flower visits provided shown a visitor network enrich scarce data associations between wild plants Region. Bees were sampled April October 2022–2023 with two Malaise traps nets. extracted amplify sequences mitochondrial gene Cyotochrome oxydase I, then compared those BOLD using identification tool constructing neighbor-joining phylogenetic trees. Seventy-six different species belonging 29 genera six families collected identified. A total 212 COI obtained for 61 species, many had not yet been sequenced Italian populations. Five taxa Sardo-Corsican endemics, whereas newly recorded Sardinia. Finally, we highlight potential taxonomic issues new visit records, emphasizing need further research better understand ecology group insects toward conservation.

Language: Английский

Citations

0