Common Ancestry of the Id Locus: Genetic Clues and Polygenic Possibilities DOI Open Access
Ashutosh Sharma, Nagarjun Vijay

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 29, 2023

Abstract The diversity in dermal pigmentation and plumage color among domestic chickens is striking, with Black Bone Chickens (BBC) particularly notable for their intense melanin hyperpigmentation. This unique trait driven by a complex chromosomal rearrangement on chromosome 20 at the Fm locus, resulting overexpression of EDN3 (a gene central to melanocyte regulation). In contrast, inhibition regulated Id locus. Although prior studies using genetic crosses, GWAS, expression analysis have investigated underpinnings its precise location functional details remain elusive. Our study aims precisely locate identify associated rearrangements candidate genes influencing pigmentation, examine ancestral status locus BBC breeds. Using public genomic data from non-BBC breeds, we refined ∼1.6 Mb region that co-localizes Z amplicon repeat units distal end q-arm within 10.36 inversion Silkie BBC. Phylogenetic population structure analyses reveal shares common ancestry across all much like Selection signatures highly differentiated BBC-specific SNPs MTAP position it as prime CCDC112 additional genes, suggesting possible polygenic nature. results suggest shared breeds may function supergene cluster shank variation.

Language: Английский

Common Ancestry of the Id Locus: Chromosomal Rearrangement and Polygenic Possibilities DOI
Ashutosh Sharma, Nagarjun Vijay

Journal of Molecular Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

Citations

1

Klumpy: A tool to evaluate the integrity of long‐read genome assemblies and illusive sequence motifs DOI Creative Commons
Giovanni Madrigal, Bushra Fazal Minhas, Julian Catchen

et al.

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 27, 2024

Abstract The improvement and decreasing costs of third‐generation sequencing technologies has widened the scope biological questions researchers can address with de novo genome assemblies. With increasing number reference genomes, validating their integrity minimal overhead is vital for establishing confident results in applications. Here, we present Klumpy, a tool detecting visualizing both misassembled regions assembly genetic elements (e.g. genes) interest set sequences. By leveraging initial raw reads combination respective assembly, illustrate Klumpy's utility by investigating antifreeze glycoprotein ( afgp ) loci across two icefishes, searching reported absent gene northern snakehead fish, scanning genomes mudskipper bumblebee regions. In former cases, were able to provide support noncanonical placement an locus icefishes locate missing gene. Furthermore, our scans identify unmappable putative repetitive element shared among several species bees.

Language: Английский

Citations

4

Detecting Assembly Errors With Klumpy: Building Confidence in Your Daily Genomic Analysis DOI
Isheng Jason Tsai

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

In the realm of genome assembly, even minor errors can send researchers down to rabbit holes unintended misinterpretation. Enter Klumpy—a tool designed help detecting these elusive mistakes before they cause significant problems. By providing detailed, region‐specific assessments and an intuitive visualisation platform, Klumpy (Madrigal, et al. 2024) empowers pinpoint resolve potential issues with precision, paving way for more reliable downstream analyses discoveries.

Language: Английский

Citations

0

Common Ancestry of the Id Locus: Genetic Clues and Polygenic Possibilities DOI Open Access
Ashutosh Sharma, Nagarjun Vijay

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 29, 2023

Abstract The diversity in dermal pigmentation and plumage color among domestic chickens is striking, with Black Bone Chickens (BBC) particularly notable for their intense melanin hyperpigmentation. This unique trait driven by a complex chromosomal rearrangement on chromosome 20 at the Fm locus, resulting overexpression of EDN3 (a gene central to melanocyte regulation). In contrast, inhibition regulated Id locus. Although prior studies using genetic crosses, GWAS, expression analysis have investigated underpinnings its precise location functional details remain elusive. Our study aims precisely locate identify associated rearrangements candidate genes influencing pigmentation, examine ancestral status locus BBC breeds. Using public genomic data from non-BBC breeds, we refined ∼1.6 Mb region that co-localizes Z amplicon repeat units distal end q-arm within 10.36 inversion Silkie BBC. Phylogenetic population structure analyses reveal shares common ancestry across all much like Selection signatures highly differentiated BBC-specific SNPs MTAP position it as prime CCDC112 additional genes, suggesting possible polygenic nature. results suggest shared breeds may function supergene cluster shank variation.

Language: Английский

Citations

0