Estuarine Coastal and Shelf Science, Journal Year: 2024, Volume and Issue: unknown, P. 109097 - 109097
Published: Dec. 1, 2024
Language: Английский
Estuarine Coastal and Shelf Science, Journal Year: 2024, Volume and Issue: unknown, P. 109097 - 109097
Published: Dec. 1, 2024
Language: Английский
Journal of Limnology, Journal Year: 2025, Volume and Issue: 84
Published: Feb. 10, 2025
We report the results of first survey bdelloid rotifers, microscopic aquatic animals, in continental Norway, collected from coastal ponds Southern part country Autumn 2021, using a morphological approach species identification. Out 25 ponds, 19 were found, ten water samples and another nine limno-terrestrial habitats just above waterline ponds. Seven are new records for mainland Norway. Three could be identified to genus level only may novel taxa, not Norway but science: further studies needed on these animals clarify their taxonomic identity. also obtained COI sequences 194 eight compare them with what is known GenBank phylogenetic context confirm reliability This data contributes our understanding biogeographic diversity rotifers In addition, newly available increase coverage reference library future employing DNA metabarcoding.
Language: Английский
Citations
0Integrative Zoology, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
DNA metabarcoding is revolutionizing biodiversity research by providing rapid and efficient ways of collecting species occurrence data. However, it has not yet been effectively applied to many taxonomic groups, mainly due a significant lack reference sequences dedicated protocols. One such group the tardigrades-a charismatic phylum microinvertebrates known for their extremophilic cryptobiotic capabilities. In this study, we provide first curated database 3194 tardigrade COI sourced from public databases supplemented with newly produced barcodes. We demonstrate in action optimized PCR primers sample processing protocol using 78 samples collected Poland Italy. The revealed presence more than hundred operational units classified as Tardigrada, representing 23 genera. compared results morphological survey, which same genera, but lower number species-level taxa identified morphologically. observed congruent patterns richness composition between surveys both within-sample regional fauna levels. had higher discriminatory power, revealing cryptic diversity, distinguishing belonging taxonomically challenging complexes. By combining were able find rare taxa, including novel biogeographic records putative new science, showing also that approach can be extremely powerful effective meiofauna research.
Language: Английский
Citations
0Biodiversity Data Journal, Journal Year: 2024, Volume and Issue: 12
Published: Sept. 26, 2024
Molecular tools used for the identification of species are heavily reliant on reference DNA sequences and taxonomic annotation. Despite this, there large gaps in availability many groups different parts globe. Here, a barcode library Hymenoptera New Zealand is presented, based COI region 3,145 assigned to 837 BINs which represent 231 genera 236 species. This study provides approximately 25% 42% Zealand. However, when combined with previously deposited BOLD (a further 170 genera), barcodes available 73% Hymenopteran genera. To increase coverage, future efforts need focus predominantly taxa from seven families (Encyrtidae, Pteromalidae s.l., Mymaridae, Eulophidae, Diapriidae, Braconidae Platygastridae). database facilitates DNA-based use both revisions biodiversity monitoring.
Language: Английский
Citations
1Estuarine Coastal and Shelf Science, Journal Year: 2024, Volume and Issue: 313, P. 109092 - 109092
Published: Dec. 17, 2024
Language: Английский
Citations
1Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 8, 2024
Assessments of biodiversity and ecosystem status can benefit from DNA metabarcoding as a means to streamline sample processing specimen identification. Moreover, the fixation medium instead precious material introduces straightforward protocols that allow subsequent focus on certain organisms detected among preserved specimens. In this study, we present proof concept via analysis freshwater invertebrate samples Tatra Mountain lakes (Slovakia). Besides highlighting match between lake-specific environmental conditions results our fixative metabarcoding, observed an option fine-tune time: prefer two weeks over day or month. This effect emerged presence/absence individual taxa rather than coarse per-sample records taxonomic richness, demonstrating studies—and efforts optimize their protocols—can use robust metrics explore even subtle trends. We also provide evidence might better capture large species terrestrial meiofauna.
Language: Английский
Citations
1Estuarine Coastal and Shelf Science, Journal Year: 2024, Volume and Issue: unknown, P. 109097 - 109097
Published: Dec. 1, 2024
Language: Английский
Citations
0