COI Metabarcoding With a Curated Reference Database and Optimized Protocol Provides a Reliable Species‐Level Diversity Assessment of Tardigrades

Integrative Zoology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

DNA metabarcoding is revolutionizing biodiversity research by providing rapid and efficient ways of collecting species occurrence data. However, it has not yet been effectively applied to many taxonomic groups, mainly due a significant lack reference sequences dedicated protocols. One such group the tardigrades-a charismatic phylum microinvertebrates known for their extremophilic cryptobiotic capabilities. In this study, we provide first curated database 3194 tardigrade COI sourced from public databases supplemented with newly produced barcodes. We demonstrate in action optimized PCR primers sample processing protocol using 78 samples collected Poland Italy. The revealed presence more than hundred operational units classified as Tardigrada, representing 23 genera. compared results morphological survey, which same genera, but lower number species-level taxa identified morphologically. observed congruent patterns richness composition between surveys both within-sample regional fauna levels. had higher discriminatory power, revealing cryptic diversity, distinguishing belonging taxonomically challenging complexes. By combining were able find rare taxa, including novel biogeographic records putative new science, showing also that approach can be extremely powerful effective meiofauna research.

Language: Английский

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Tree hollow parameters and their effects on saproxylic arthropod diversity: A multi‐taxon approach

Insect Conservation and Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Abstract Tree hollows are keystone structures for biodiversity in forest ecosystems, promoting diverse saproxylic communities. However, intensive management central Europe has made them rare. Consequently, arthropods inhabiting tree among the most endangered invertebrates. Most studies on arthropod diversity have focused beetles. To understand this complex habitat, non‐beetle groups and their habitat requirements must also be investigated. We collected emerging from 40 beech trees 2014 a subset of 23 2015 managed southern Germany using emergence traps. DNA metabarcoding identified arthropods, assessing influence hollow parameters structure α‐ β‐diversity species‐rich taxa. Parameters influencing differed by year The important factors positively affecting α‐diversity were volume, circumference entrance area/lateral surface, while area decomposition negatively affected α‐diversity. Beta‐diversity was influenced circumference, (dipterans: dead wood amount, spiders: nearby hollows). These results highlight importance heterogeneous structures, emphasising need conservation to support highly

Language: Английский

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Latitudinal gradients of biodiversity and ecosystem services in protected and non-protected oak forest areas can inform climate smart conservation

Geography and sustainability, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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Advances in insect biomonitoring for agriculture and forestry: A synthesis on a multifaceted special issue of Agricultural and Forest Entomology

Agricultural and Forest Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

12 December 2024: This article published in Early View error. The is under embargo and will republish on 7 January 2025.

Language: Английский

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