Next‐Generation Snow Leopard Population Assessment Tool: Multiplex‐PCR SNP Panel for Individual Identification From Faeces DOI Open Access
Katherine A. Solari, S. Ahmad, Ellie E. Armstrong

et al.

Molecular Ecology Resources, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

ABSTRACT In recent years, numerous single nucleotide polymorphism (SNP) panel methods to genotype non‐invasive faecal samples have been developed. However, none of these existing fit all the criteria necessary make a SNP broadly usable for conservation projects in any country—cost effective, streamlined lab protocol and user‐friendly open‐source bioinformatics protocols design analysis. Here, we present such method display its utility by developing multiplex PCR conducting individual ID snow leopards, Panthera uncia , from samples. The consists 144 SNPs utilises next‐generation sequencing technology. We validate our with paired tissue zoo individuals, showing minimum 96.7% accuracy allele calls per run. then generate data 235 field‐collected across Pakistan show that can reliably identify individuals low‐quality unknown age is robust contamination. also has capability first‐order relatives among sampled provides insights into geographic origin This will empower leopard research community their efforts assess local global population sizes. More broadly, development be used species interest which adequate genomic reference available.

Language: Английский

Next‐Generation Snow Leopard Population Assessment Tool: Multiplex‐PCR SNP Panel for Individual Identification From Faeces DOI Open Access
Katherine A. Solari, S. Ahmad, Ellie E. Armstrong

et al.

Molecular Ecology Resources, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

ABSTRACT In recent years, numerous single nucleotide polymorphism (SNP) panel methods to genotype non‐invasive faecal samples have been developed. However, none of these existing fit all the criteria necessary make a SNP broadly usable for conservation projects in any country—cost effective, streamlined lab protocol and user‐friendly open‐source bioinformatics protocols design analysis. Here, we present such method display its utility by developing multiplex PCR conducting individual ID snow leopards, Panthera uncia , from samples. The consists 144 SNPs utilises next‐generation sequencing technology. We validate our with paired tissue zoo individuals, showing minimum 96.7% accuracy allele calls per run. then generate data 235 field‐collected across Pakistan show that can reliably identify individuals low‐quality unknown age is robust contamination. also has capability first‐order relatives among sampled provides insights into geographic origin This will empower leopard research community their efforts assess local global population sizes. More broadly, development be used species interest which adequate genomic reference available.

Language: Английский

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