Single‐cell sequencing unveils the impact of aging on the progenitor cell diversity in the telencephalon of the female killifish N. furzeri
Aging Cell,
Journal Year:
2024,
Volume and Issue:
23(10)
Published: July 1, 2024
Abstract
The
African
turquoise
killifish
(
Nothobranchius
furzeri
)
combines
a
short
lifespan
with
spontaneous
age‐associated
loss
of
neuro‐regenerative
capacity,
an
intriguing
trait
atypical
for
teleost.
impact
aging
on
the
cellular
composition
adult
stem
cell
niches,
leading
to
this
dramatic
decline
in
postnatal
neuro‐
and
gliogenesis,
remains
elusive.
Single‐cell
RNA
sequencing
telencephalon
young
female
short‐lived
GRZ‐AD
strain
unveiled
progenitors
glial
non‐glial
nature,
different
excitatory
inhibitory
neuron
subtypes,
as
well
non‐neural
types.
Sub‐clustering
identified
four
radial
glia
(RG)
types,
two
progenitor
(NGP)
intermediate
(intercell)
states.
Two
astroglia‐like,
one
ependymal,
neuroepithelial‐like
(NE)
RG
subtype
were
found
at
locations
forebrain
line
their
role,
while
proliferative,
active
NGPs
spread
throughout.
Lineage
inference
pointed
NE‐RG
start
intercessor
populations
glio‐
neurogenesis.
Upon
aging,
single‐cell
revealed
major
perturbations
proportions
astroglia
intercell
states,
molecular
signatures
specific
including
altered
MAPK,
mTOR,
Notch,
Wnt
pathways.
This
catalog
regeneration‐competent
telencephalon,
combined
evidence
aging‐related
transcriptomic
changes,
presents
useful
resource
understand
basis
age‐dependent
neuroplasticity.
data
is
also
available
through
online
database
killifishbrain_scseq
).
Language: Английский
Brain aging and rejuvenation at single-cell resolution
Neuron,
Journal Year:
2025,
Volume and Issue:
113(1), P. 82 - 108
Published: Jan. 1, 2025
SummaryBrain
aging
leads
to
a
decline
in
cognitive
function
and
concomitant
increase
the
susceptibility
neurodegenerative
diseases
such
as
Alzheimer's
Parkinson's
diseases.
A
key
question
is
how
changes
within
individual
cells
of
brain
give
rise
age-related
dysfunction.
Developments
single-cell
"omics"
technologies,
transcriptomics,
have
facilitated
high-dimensional
profiling
cells.
These
technologies
led
new
comprehensive
characterizations
at
resolution.
Here,
we
review
insights
gleaned
from
omics
studies
aging,
starting
with
cell-type-centric
overview
age-associated
followed
by
discussion
cell-cell
interactions
during
aging.
We
highlight
provide
an
unbiased
view
different
rejuvenation
interventions
comment
on
promise
combinatorial
approaches
for
brain.
Finally,
propose
directions,
including
models
neural
stem
focal
point
rejuvenation.
Language: Английский
Multi-tissue transcriptomic aging atlas reveals predictive aging biomarkers in the killifish
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Abstract
Aging
is
associated
with
progressive
tissue
dysfunction,
leading
to
frailty
and
mortality.
Characterizing
aging
features,
such
as
changes
in
gene
expression
dynamics,
shared
across
tissues
or
specific
each
tissue,
crucial
for
understanding
systemic
local
factors
contributing
the
process.
We
performed
RNA-sequencing
on
13
at
6
different
ages
African
turquoise
killifish,
shortest-lived
vertebrate
that
can
be
raised
captivity.
This
comprehensive,
sex-balanced
‘atlas’
dataset
reveals
varying
strength
of
sex-age
interactions
killifish
identifies
age-altered
biological
pathways
are
evolutionarily
conserved.
Demonstrating
utility
this
resource,
we
discovered
head
kidney
exhibits
a
myeloid
bias
during
aging,
phenomenon
more
pronounced
females
than
males.
In
addition,
developed
tissue-specific
‘transcriptomic
clocks’
identified
biomarkers
predictive
chronological
age.
show
importance
sex-specific
clocks
selected
use
evaluate
dietary
intervention
killifish.
Our
work
provides
comprehensive
resource
studying
dynamics
powerful
model.
Language: Английский
Early-life growth and cellular heterogeneity in the short-lived African turquoise killifish telencephalon
Biology Open,
Journal Year:
2025,
Volume and Issue:
14(4)
Published: April 15, 2025
ABSTRACT
The
African
turquoise
killifish
(Nothobranchius
furzeri)
is
becoming
a
favorable
model
for
neurobiological
research.
combination
of
short
lifespan
and
declining
neuroregenerative
capacity
upon
aging
makes
it
ideally
suited
research
on
brain
regeneration.
A
remarkable
cellular
diversity
up
the
young-adult
telencephalon,
characterized
by
highly
proliferative
non-glial
progenitors
spatially
distinct
radial
glia
subtypes.
In
contrast
to
relatively
slow
embryonic
development,
hatching
followed
period
accelerated
growth,
in
which
experiences
rapid
expansion
maturation.
this
study,
we
quantified
growth
progression
maturation
telencephalon
during
early
post-embryonic
development.
We
discovered
that,
similar
zebrafish,
neuro-epithelial
cells
abut
neurogenic
niches
from
life
onwards.
Spatial
data
revealed
qualitative
quantitative
differences
along
anterior-posterior
axis
between
pallial
subpallial
regions
terms
pace.
confirmed
generation
GABAergic
neurons
niche
glutamatergic
two
niches.
Our
further
showed
more
widespread
appearance
inhibitory
at
compared
zebrafish.
Language: Английский