Expression of Aquaglyceroporins in Spermatozoa from Wild Ruminants Is Influenced by Photoperiod and Thyroxine Concentrations
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(6), P. 2903 - 2903
Published: March 8, 2022
This
work
identified
the
presence
of
AQPs
in
frozen-thawed
sperm
wild
ruminants
and
assessed
influence
interaction
between
photoperiod
thyroxine
on
AQP
expression,
testosterone
secretion.
Thyroxine
melatonin
were
administered
to
ibexes.
In
a
second
experiment,
performed
mouflons,
circulating
was
reduced
via
treatment
with
propylthiouracil
(PTU),
an
artificial
long
day
(LD)
established.
ibexes,
increased
blood
plasma
concentration,
cryoresistance
ratio
(CR)
for
viability
intact
acrosome,
percentage
AQP7
acrosome
AQP3
AQP10
midpiece.
neither
PTU
treatment,
LD,
nor
combination
both
affected
CR
any
variable.
The
post-acrosome
region
but
decreased
tail
LD+PTU
group.
principal
piece
endpiece
lower
PTU+LD
group
than
control
LD
groups.
photoperiod/melatonin
expression
might
be
indirectly
exerted
through
changes
thus
ultimately
affect
cryoresistance.
Language: Английский
Review: Sperm cryopreservation in wild small ruminants: morphometric, endocrine and molecular basis of cryoresistance
animal,
Journal Year:
2023,
Volume and Issue:
17, P. 100741 - 100741
Published: May 1, 2023
Reproductive
technologies
can
help
to
protect
wild
ruminant
species
from
becoming
extinct.
In
addition,
the
decline
in
some
game
has
also
raised
interest
reproductive
increase
number
of
animals
that
be
produced.
Most
biobanking
efforts
have
focused
on
developing
effective
protocols
for
preserving
sperm,
oocytes,
and
embryos.
Cryopreservation
sperm
remains
least
invasive
method
cheapest
procedure
germplasm
storage.
Over
last
few
years,
several
biotechnologies
been
developed
beyond
conventional
freezing
spermatozoa.
These
include
ultra-rapid
techniques.
Nevertheless,
fertility
results
after
artificial
insemination
using
frozen-thawed
spermatozoa
are
not
always
acceptable
small
ruminants.
Moreover,
these
technological
met
variable
success
related
sample's
origin
(epididymal
retrieved
postmortem
or
ejaculated)
season
sample
collection
Epididymal
shows
higher
cryoresistance
than
ejaculated
sperm.
Changes
proteome
between
epididymal
seem
contribute
this
different
cryotolerance.
The
role
endocrine
status
studied
better
understand
underlying
mechanism
annual
variation
cryoresistance.
Seasonal
changes
testosterone
prolactin
involved
cryoresistance;
recovery
cryopreservation
recommended
around
end
rutting
season,
when
good
quality
samples
still
obtained,
levels
already
decreased,
concentrations
remain
low.
mechanisms
hormone
action
freezability
well
known.
Still,
it
suggested
affects
cell
proliferation
testis,
during
spermatogenesis,
membrane
properties
cells
their
transit
through
tract,
which
might
influence
Recent
studies
revealed
expression
aquaporins
ruminants
could
androgen-related
seasonal
Along
with
spermatozoa,
testicular
tissue
may
provide
a
suitable
source
male
gametes,
an
alternative
establishing
banks
semen
cannot
collected
whatever
reason.
Language: Английский
The role of Testosterone in male reproductive system (review)
M. А. Maksimova,
No information about this author
Е. А. Корочкина
No information about this author
Legal regulation in veterinary medicine,
Journal Year:
2024,
Volume and Issue:
3, P. 51 - 56
Published: Oct. 13, 2024
Testosterone
is
a
hormone
that
predominantly
formed
in
Leydig
cells
adjacent
to
the
seminal
tubules
of
testis,
ovaries
and
adrenal
cortex
[1,
5,
6].
production
regulated
by
hypothalamic-pituitary
system
under
guidance
gonadotropin-releasing
(GnRH)
luteinizing
(LH),
between
which
there
negative
feedback
[5-6].
In
addition
GnRH
LH,
testosterone
also
interconnected
with
other
hormones:
melatonin
[14],
oxytocin
[15],
thyroid
hormones
[16].
has
great
effect
on
body
animals.
Thus,
it
promotes
growth
maturation
bone
tissue
[17],
prevents
oxidative
damage
brain
[19-20],
affects
metabolism
[21],
as
well
animal
behavior
[23].
addition,
studies
have
noted
important
role
spermatogenesis.
Testosterone,
binding
androgen
receptor,
regulates
proliferation
Sertoli
cells,
maintains
integrity
blood-brain
barrier,
meiotic
post-meiotic
processes
germ
[27-30].
The
sperm
cryoresistance
noted,
however,
some
authors
emphasize
[32-
33],
while
others
point
beneficial
[34].
females,
testosterone,
together
androgens,
binds
receptors,
changes
activity
interacts
factors
[36].
Studies
show
hormone,
able
stimulate
progesterone
[37].
It
known
many
influence
dynamics
blood.
For
example,
passage
age,
androgens
blood
decrease
bulls
[41],
adult
rams,
content
1.8
times
higher
than
younger
individuals
[43].
Another
factor
worth
noting
feeding:
diet
protein
contributes
positive
animals,
increases
levels
[44-45].
heat
stress
suppresses
expression
enzymes
involved
synthesis
[47].
Language: Английский
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation
J. Cerdeira,
No information about this author
C. Castaño,
No information about this author
J. F. Pérez
No information about this author
et al.
Animal Reproduction Science,
Journal Year:
2021,
Volume and Issue:
235, P. 106887 - 106887
Published: Nov. 14, 2021
Language: Английский
Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)
Zoo Biology,
Journal Year:
2022,
Volume and Issue:
42(3), P. 364 - 370
Published: Oct. 14, 2022
In
both
captive
wildlife
and
production
animals
is
important
to
develop
strategies
for
population
control.
Immunization
against
GnRH
an
easy
inexpensive
immunocastration
method
that
reduces
the
concentration
of
testosterone
decreases
sperm
quality.
However,
its
effectiveness
depends
on
species
repetition
treatment.
This
study
aimed
compare
a
single
treatment
(initial
immunization
plus
booster
with
Improvac)
vs
repeated
(six
doses
inhibit
testicular
function
maintain
contraceptive
status
during
long
periods
in
bucks.
Three
Dwarf
bucks
(Capra
hircus)
received
two
Improvac,
first
Week
0,
4
weeks
later
(single
immunization,
group
SI)
while
three
one
dose
Improvac
every
6
months
3
consecutive
years
(repeated
RI).
The
other
remained
untreated
(control
bucks,
CON).
Bucks
from
RI
had
greater
decrease
scrotal
circumference,
concentration,
male
odor
intensity,
quality
than
SI
there
were
no
differences
between
CON
any
variables
studied.
Overall,
decreased
although
did
not
produce
complete
infertility.
produced
more
intensive
negative
effects,
indicating
strength
effects
rapidly
lost
Language: Английский
The freezability of Mediterranean buffalo sperm is associated with lysine succinylation and lipid metabolism
Xi Luo,
No information about this author
Shihai Huang,
No information about this author
Mingming Liang
No information about this author
et al.
The FASEB Journal,
Journal Year:
2022,
Volume and Issue:
36(12)
Published: Nov. 5, 2022
Semen
cryopreservation
is
used
for
the
propagation
of
variety
among
species
and
domestic
breeding.
Mitochondria
are
implicated
in
sperm
freezability,
their
proteins
prone
to
succinylation,
but
relationship
between
freezability
mitochondrial
protein
succinylation
unclear.
In
this
study,
six
bulls
were
classified
as
having
good
or
poor
ejaculates
(GFE
PFE,
each
3
bulls).
The
fresh
membrane
potential
(MMP)
pan
level
two
groups
first
detected.
Then
lysine
succinylome
fatty
acid
content
analyzed
using
label-free
LC–MS/MS
GC–MS/MS
multiple
reaction
monitoring
(MRM)
modes,
respectively.
results
indicated
that
GFE
had
significantly
higher
MMPs
than
PFE
group
(p
<
0.05).
A
total
1393
sites
corresponding
426
assessed
5
succinylated
peptides
markedly
upregulated,
while
downregulated
(FC
>
2.0
–
0.5
p-value
0.05)
when
compared
group.
Forty-six
identified
have
consistent
presence/absence
expression.
upregulated
enriched
lipid
metabolic
processes.
31
acids
further
subjected
quantitative
analysis
which
23
including
arachidic
(C20:0),
linolenic
(C18:3n3),
docosahexaenoic
(C22:6n3)
decreased
with
These
suggest
can
potentially
influence
Mediterranean
buffaloes
through
metabolism.
This
novel
study
provides
our
understanding
molecular
basis
mechanism
freezability.
Language: Английский
Detection of Androgen Receptors in Spermatozoa of Small Ruminants: A Putative Modulation Pathway for Cryoresistance Through AQP3
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 11972 - 11972
Published: Nov. 7, 2024
This
work
was
aimed
to
identify
androgen
receptors
(AR)
in
the
spermatozoa
of
wild
and
domestic
ruminants
assess
effect
testosterone
on
sperm
localization
aquaporin-3
(AQP3)
cryopreservation
process.
Sperm
samples
from
species
were
incubated
with
(T
group),
1,3-propanediol
(PDO
phloretin
(PHL
PDO+T
group,
PHL+T
group.
Western
blot
identified
presence
AR
as
a
single
band
about
48
KDa.
Immunolabelling
located
equatorial
segment
head.
In
mouflons,
cryoresistance
ratio
for
acrosome
integrity
lower
(
Language: Английский
Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge
Veterinary medicine and science,
Journal Year:
2023,
Volume and Issue:
unknown
Published: Sept. 19, 2023
Current
sperm
cryopreservation
protocols
for
domestic
and
wild
mammals
aim
to
minimize
the
cryogenic
damage
caused
by
cell
dehydration,
ice
formation,
osmotic
stress.
The
optimization
of
include
use
different
synthetic
nonsynthetic-based
extenders
supplemented
with
additives
(e.g.,
egg
yolk,
coconut
water,
etc.)
antioxidants
melatonin,
L-carnitine,
caffeine,
resveratrol,
that
protect
plasmalemma,
acrosome,
mitochondria
against
detrimental
effects
process.
Furthermore,
penetrating
glycerol,
ethylene
glycol,
dimethylformamide,
nonpenetrating
sucrose
trehalose)
cryoprotectant
agents
(CPAs)
or
their
combination
should
be
investigated
during
freezing
process
in
slow
ultra-rapid
procedures.
Finally,
new
focus
on
curves
initial
cooling
rates
allow
optimal
dehydration
adequate
hydration
thawing.
suitable
interaction
all
these
factors
will
a
subpopulation
survive
integrity
fertilizing
capacity,
contributing
improvement
efficiency
genetic
resource
management
development
germplasm
banks
support
preservation
diversity
animals.
Language: Английский
Effect of Testosterone, Dihydrotestosterone, Estradiol and Cortisol on the Quality of Fresh and Cryopreserved Stallion Sperm
Journal of Experimental Biology and Agricultural Sciences,
Journal Year:
2022,
Volume and Issue:
10(3), P. 619 - 627
Published: June 26, 2022
The
effect
of
steroid
hormones
on
the
quality
fresh
and
cryopreserve
sperm
has
not
been
fully
understood.
This
study
aimed
to
evaluate
testosterone,
dihydrotestosterone,
estradiol,
cortisol
cryopreserved
stallion
sperm.
was
conducted
40
Equus
caballus
stallions,
including
Arab
(n=20),
Oryol
trotting
(n=4),
Standardbred
Soviet
Heavy
Draft
(n=12)
breeds.
average
age
experimental
animals
9.9
±
0.7
years.
We
determined
standard
indicators
in
concentration
blood
plasma
stallions.
Results
suggested
a
negative
correlation
between
level
testosterone
with
total
(r=-0.41;
p<0.01)
progressive
(r=-0.44;
motility
as
well
(r=-0.38;
p<0.05
r=-0.39;
correspondingly).
While
estradiol
showed
positive
survival
rate
(r=0.35;
p<0.05)
(r=0.33;
Further,
stallions
did
show
any
statistically
significant
correlations
qualitative
characteristics
A
relationship
found
dihydrotestosterone
volume
ejaculate
(r=0.37;
number
(r=0.43;
p<0.01).
can
be
concluded
that
have
different
effects
their
should
considered
when
selecting
for
cryopreservation
Language: Английский