Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation DOI Open Access
Meghan M. Moran, Jun Li, Quan Shen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Disrupted intestinal epithelial barrier function has been proposed to be integral rheumatoid arthritis (RA) progression and pathogenesis. To further define the molecular pathways in synovial inflammation response of tissues, we have used a rat model mono-joint inflammatory arthritis, induced by intra-articular injection Complete Freund’s adjuvant (CFA). The predominant single into knee joint resulted rapid reproducible formation fibrotic myeloid-infiltrated pannus. Our aim was determine how including proximal distal ileum colon, responded changes synovium temporally coordinated manner comparing their transcriptomic landscapes using RNASeq analyses. We confirmed timeline swelling measurement, increased fluid levels bikunin (a component both acute phase protein pre-alpha-inhibitor inter-alpha-inhibitor) demonstrated self-correcting trabecular cortical bone CFA challenge. Intestine-specific responses were monitored 16S microbiome amplicon sequencing, histopathology for mucus layer integrity, immune cell immunohistochemistry. present data that shows tissue displays an allostatic region specific. primarily with secretion silencing T-cell specific pathways, whereas colon showed transient upregulation macrophages, broader suppression related metabolic pathway transcripts. Interestingly, many neuropathways activated early but then suppressed later colon. There only insignificant fecal composition or post-CFA administration. In summary, our show first time following induction minimal microbiome. results help clarify tissues stresses accompany pathogenesis diseases.

Language: Английский

Letter: Allostatic load and adverse prognosis in inflammatory bowel disease—Need more evidence DOI Open Access
Lingyu Xu, Guan Chen, Long Zhao

et al.

Alimentary Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: 60(10), P. 1483 - 1484

Published: Oct. 5, 2024

LINKED CONTENT This article is linked to Zhao et al papers. To view these articles, visit https://doi.org/10.1111/apt.18217 and https://doi.org/10.1111/apt.18303

Language: Английский

Citations

1
Editorial: Allostatic Load and Inflammatory Bowel Disease DOI Open Access

J.L. Mendoza,

James Irwin

Alimentary Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

The construct that chronic stress causes illness, and reducing improves health, is widely accepted heavily researched. Zhao et al. demonstrate an association between allostatic load biomarker panel (ALBP; blood pressure, heart rate, HbA1c, cholesterol, waist-to-hip ratio [WHR], CRP, IGF-1, creatinine) the incidence severity of inflammatory bowel disease (IBD), in a cohort from UK Biobank [1]. Allostasis refers to one's ability maintain physiological stability response environmental through neuroendocrine system. Allostatic (AL) cumulative burden placed on body due dysregulation stress-response systems. measurable primary mediators AL are cortisol, serum dehydroepiandrosterone sulfate (DHEAS), adrenaline noradrenaline. original secondary outcome measures (physiological parameters may be altered by stress) systolic diastolic high-density lipoproteins, total glycosylated haemoglobin abdominal obesity (measured ratio) [2]. Many studies have endeavoured link with cardiovascular disease, general functional decline, cognitive decline development metabolic syndrome. Two major issues commonly occur: firstly, difficulty demonstrating causal relationship measured biomarkers secondly, quantifying longitudinal AL. Both these present this study. utilised Biobank, which has recorded environmental, lifestyle genetic data 500,000 participants collected since 2006. Participants more than thirty key biochemistry markers at baseline. An ALBP was defined for study using single point time. high scores were shown greater risk developing IBD. correlation outcomes robust. However, assumed. Primary not included ALBP. There no convincing literature exposure [3]. Notably, there significant overlap criteria Out context, could interpreted as syndrome Additionally, it difficult capture true AL, accumulation stress, baseline data. This provides clear evidence specific associated other studies, unclear. line research ultimately asks whether reduction change lower IBD incidence. In order answer question, standardised validated definition required, then needs correlated Given temporal variability timeframe required make observations outcome, inherent observational nature design human research, challenging achieve. A yet determined. Jeli Mendoza: writing – review editing. James Irwin: article linked al papers. To view articles, visit https://doi.org/10.1111/apt.18217 https://doi.org/10.1111/apt.18414. Data sharing applicable new created or analyzed

Language: Английский

Citations

1
Letter: Allostatic Load and Adverse Prognosis in Inflammatory Bowel Disease—Need More Evidence. Authors' Reply DOI

Jianhui Zhao,

Erxu Xue, Zhanju Liu

et al.

Alimentary Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: 60(10), P. 1485 - 1486

Published: Oct. 5, 2024

LINKED CONTENT This article is linked to Zhao et al papers. To view these articles, visit https://doi.org/10.1111/apt.18217 and https://doi.org/10.1111/apt.18249 .

Language: Английский

Citations

0
Editorial: Allostatic Load and Inflammatory Bowel Disease. Authors' Reply DOI

Jianhui Zhao,

Erxu Xue, Zhanju Liu

et al.

Alimentary Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

We thank Drs Mendoza and Irwin for the insightful editorial on our paper [1, 2]. Allostatic load (AL), which represents cumulative wear tear multiple organs tissues resulting from various stressors throughout life, may serve as a pivotal lever to explore association between chronic stress development progression of inflammatory bowel disease (IBD) [3]. However, several points merit further discussion. First, effectively assessing characterising AL has been widely discussed. Since concept was introduced, research linking it health outcomes surged in past 30 years. there remains lack consensus regarding assessment characterisation methods, severely limits ability make comparisons across studies [4]. The system-specific scoring method regarded significant advancement Furthermore, clinimetrics extend beyond biomarkers have developed describe or measure symptoms, physical signs other clinical phenomena [5]. Second, most relies baseline static measurements biomarkers, making difficult capture dynamic changes over time. emphasises preclinical state; therefore, monitoring allostatic biomarker panel (ALBP) time can more utilise this information identify individuals at greater risk developing specific diseases enable early intervention, reflects true utility Third, although five dimensions metabolic syndrome (MetS; i.e., obesity, triglycerides, blood pressure, glucose HDL-cholesterol) are included ALBP calculation, key difference is that MetS uses thresholds diagnosis, while relative levels general population, mainly indicating state [6]. Beyond cardiovascular systems MetS, includes sympathetic nervous system, inflammatory/immune system renal function, all linked higher (Figure 1A). Finally, lifestyle environmental factors influence AL. conducted supplementary analysis found an unhealthy significantly associated with elevated A systematic review supports findings [7]. Evidence lifestyles regulate stress, bidirectional relationship exists two [8]. Therefore, maintaining healthy potentially reduce by mitigating impact stress. Additionally, green residential environment lower [9], poor living environment, characterised lead exposure, household crowding, dangerous traffic riskscapes, contribute increased [10]. Considering influenced be reduced through multidimensional opportunity prevention intervention related AL, such IBD. In summary, future should validate standardised measurement assess using longitudinal repeated measures data accurately evaluate concerning occurrence investigations health-promoting mitigate Jianhui Zhao: conceptualization, formal analysis, writing – original draft. Erxu Xue: curation. Zhanju Liu: editing, supervision. Xue Li: supervision, editing. authors' declarations personal financial interests unchanged those article [1]. This Zhao et al papers. To view these articles, visit https://doi.org/10.1111/apt.18217 https://doi.org/10.1111/apt.18325 support study available corresponding author upon reasonable request.

Language: Английский

Citations

0
Letter: Allostatic load—A potential key in the development of inflammatory bowel disease DOI Open Access

Maryam Kharal,

Muhammad Shahbaz,

Muhammad Salman Nadeem

et al.

Alimentary Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: 60(10), P. 1505 - 1506

Published: Oct. 17, 2024

LINKED CONTENT This article is linked to Zhao et al paper. To view this article, visit https://doi.org/10.1111/apt.18217 .

Language: Английский

Citations

0
Evidence of Silencing of Intestinal Inflammatory and Immune Transcripts Following Induction of Joint Inflammation DOI Open Access
Meghan M. Moran, Jun Li, Quan Shen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Disrupted intestinal epithelial barrier function has been proposed to be integral rheumatoid arthritis (RA) progression and pathogenesis. To further define the molecular pathways in synovial inflammation response of tissues, we have used a rat model mono-joint inflammatory arthritis, induced by intra-articular injection Complete Freund’s adjuvant (CFA). The predominant single into knee joint resulted rapid reproducible formation fibrotic myeloid-infiltrated pannus. Our aim was determine how including proximal distal ileum colon, responded changes synovium temporally coordinated manner comparing their transcriptomic landscapes using RNASeq analyses. We confirmed timeline swelling measurement, increased fluid levels bikunin (a component both acute phase protein pre-alpha-inhibitor inter-alpha-inhibitor) demonstrated self-correcting trabecular cortical bone CFA challenge. Intestine-specific responses were monitored 16S microbiome amplicon sequencing, histopathology for mucus layer integrity, immune cell immunohistochemistry. present data that shows tissue displays an allostatic region specific. primarily with secretion silencing T-cell specific pathways, whereas colon showed transient upregulation macrophages, broader suppression related metabolic pathway transcripts. Interestingly, many neuropathways activated early but then suppressed later colon. There only insignificant fecal composition or post-CFA administration. In summary, our show first time following induction minimal microbiome. results help clarify tissues stresses accompany pathogenesis diseases.

Language: Английский

Citations

0