Molecular characterization and antibiotics resistance of Aeromonas species isolated from farmed African catfish Clarias gariepinus Burchell, 1822

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: Jan. 6, 2024

Abstract Background Aeromonas species are one of the most important etiologies diseases in fish farms, leading to clinical manifestation and mortality associated with public health risks. This study aimed investigate prevalence, phenotypic molecular characteristics isolated from farmed Clarias gariepinus using 16 S rRNA sequencing. Additionally, their antibiogram multiple antibiotic resistance index were determined a disc diffusion test. Results A total 230 strains 40.9% obtained diseased fish, 25% apparently healthy ones. Five different including caviae, veronii , hydrophila dhakensis enteropelogenes fully identified genetically characterized. Based on available literature, this is first report area. The phylogenetic analysis showed genetic heterogeneity distance within reference strains. resistant susceptible ciprofloxacin, gentamycin, florfenicol. species’ values varied between 0.20 0.80 farms where antibiotics intensively used. Conclusions diversity multidrug-resistant major threat production giving us more understanding epidemiology multidrug MAR greater than 0.2 use was widespread. As result, considerably increased danger spreading culture environment may impact aquaculture production. Hence there need for appropriate monitored drug usage.

Language: Английский

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Co-infections of Aeromonas veronii and Nocardia seriolae in largemouth bass (Micropterus salmoides)

Microbial Pathogenesis, Journal Year: 2022, Volume and Issue: 173, P. 105815 - 105815

Published: Oct. 7, 2022

Language: Английский

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Phenotypic and genotypic characterization of Aeromonas jandaei involved in mass mortalities of cultured Nile tilapia, Oreochromis niloticus (L.) in Brazil

Aquaculture, Journal Year: 2021, Volume and Issue: 541, P. 736848 - 736848

Published: April 30, 2021

Language: Английский

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Effects of Croton sonderianus essential oil in tambaqui (Colossoma macropomum) feeds on growth, hematology, blood chemistry, and resistance of the fish to infection with Aeromonas hydrophila

Aquaculture International, Journal Year: 2024, Volume and Issue: 32(4), P. 5149 - 5170

Published: Feb. 3, 2024

Language: Английский

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In Silico Characterization of Resistance and Virulence Genes in Aeromonas jandaei Strains Isolated from Oreochromis niloticus in Brazil

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1094 - 1094

Published: May 8, 2025

Understanding the genetic characteristics of Aeromonas jandaei in Brazilian aquaculture is crucial for developing effective control strategies against this fish pathogen. The present study conducted a genomic analysis A. strains with objective investigating their virulence potential and resistance profiles. Four isolates were subjected to sequencing, comparative analyses conjunction 48 publicly available genomes. methods employed included quality assessment, de novo assembly, annotation, antimicrobial factors. results demonstrated presence fluoroquinolone genes within core genome. Notably, these antibiotics are not authorized use Brazil, suggesting that determinants may originate from other selective pressures or horizontal gene transfer unrelated practices. identified significant mechanisms, including T2SS, T3SS, notably T6SS (vgrG3 gene), which was more prevalent isolates. Additionally, associated motility, adhesion, heavy metal identified. These findings highlight enhanced adaptability raise concerns about aquaculture, emphasizing need improved regulatory oversight strategies.

Language: Английский

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Mansoa alliacea extract improves the growth performance and innate immune response of Arapaima gigas challenged with Aeromonas hydrophila and handling stress

Acta Amazonica, Journal Year: 2023, Volume and Issue: 53(1), P. 24 - 31

Published: Jan. 27, 2023

ABSTRACT This study investigated the effects of dietary supplementation with Mansoa alliacea hydroalcoholic extracts on growth, blood and immune parameters Arapaima gigas. Fish were fed for 30 days diets enriched 0, 4, 8, 12 g kg-1 M. extract subjected to infection Aeromonas hydrophila handling stress. 8 showed significant increase in final weight, specific growth rate feed efficiency when compared other groups. Glucose, triglycerides, total proteins, globulins increased significantly fish extract, whereas albumin decreased. The number thrombocytes extract. After challenge A. stress, had higher levels glucose, globulins, albumins, an increment respiratory burst. Triglyceride dropped neutrophils increased, thrombocytes, leukocytes lymphocytes Dietary at was efficient improving innate immunity gigas, being potentially useful farming control development infections.

Language: Английский

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Molecular characterization of pathogenic Aeromonas jandaei bacteria isolated from cultured walking catfish (Clarias sp.)

Biodiversitas Journal of Biological Diversity, Journal Year: 2024, Volume and Issue: 25(3)

Published: April 8, 2024

Abstract. Mulia DS, Dwi NR, Suwarsito, Muslimin B. 2024. Molecular characterization of pathogenic Aeromonas jandaei bacteria isolated from cultured walking catfish (Clarias sp.). Biodiversitas 25: 1185-1193. spp. is capable causing fish disease, including In bacteria, virulence genes play a significant role in determining pathogenicity, while resistance influence resistivity. Therefore, this study aimed to determine molecular catfish. The purposive sampling method was used obtain diseased aquaculture ponds Banyumas District, Central Java Province, Indonesia. identification molecularly carried out based on 16S rDNA, followed by the detection aer/haem, alt, ast, flaA, lafA, and fstA, as well tet-A, strA-strB, qnrA. results analysis showed that three isolates Singasari, namely BmSL-02, BmSL-04, BmSL-07, were closely related A. strain CDC0787-80 with similarity rate query coverage 99.86% 99%, respectively. Meanwhile, Cikawung, BmCL-02, BmCL-05, BmCL-07 ATCC 49568. A total six had aer/haem fstA not found. BmSL-04 flaA gene, four lafA gene. Additionally, BmCL-05 tetA strA-strB qnrA detected all isolates. resistant bacitracin, tetracycline, gentamycin, but sensitive chloramphenicol. This provided valuable information characteristics jandaei, serving guidance effective control monitoring Indonesia, specifically Banyumas, Java.

Language: Английский

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High Genetic Diversity and Antimicrobial Resistance in Escherichia coli Highlight Arapaima gigas (Pisces: Arapaimidae) as a Reservoir of Quinolone-Resistant Strains in Brazilian Amazon Rivers

Microorganisms, Journal Year: 2022, Volume and Issue: 10(4), P. 808 - 808

Published: April 13, 2022

The increasing prevalence of multi-drug resistant (MDR) Escherichia coli in distinct ecological niches, comprising water sources and food-producing animals, such as fish species, has been widely reported. In the present study, quinolone-resistant E. isolates from Arapirama gigas, a major species Brazilian Amazon rivers farms, were characterized regarding their antimicrobial susceptibility, virulence, genetic diversity. A total forty (40) specimens A. including 20 farmed wild fish, included. Thirty-four phenotypically tested by broth microdilution, while resistance virulence genes detected PCR. Molecular epidemiology relatedness analyzed MLST PFGE typing. majority classified MDR harboring blaCTX-M, qnrA qnrB genes. Enterotoxigenic pathotype (ETEC) presented low among animals. genotyping revealed wide background, detection internationally spread clones. obtained data point out gigas reservoir aquatic ecosystems warns interference AMR strains wildlife environmental matrices.

Language: Английский

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Enterogyrus spp. (Monogenea: Ancyrocephalinae) and Aeromonas jandaei co‐infection associated with high mortality following transport stress in cultured Nile tilapia

Transboundary and Emerging Diseases, Journal Year: 2021, Volume and Issue: 69(4)

Published: Aug. 18, 2021

Monogenean infection of the internal organs is extremely rare when compared to external infections. This study describes mass mortality Nile tilapia (Oreochromis niloticus L.) originating from co-infection with Enterogyrus spp. and Aeromonas jandaei following transport stress. The first fish deaths occurred on day 1 post-transport, while cumulative reached approximately 90% by 10 post-stocking. An atypical amount pale (whitish) faeces floating surface water as well typical clinical signs motile septicemia, were reported. Adult monogeneans countless eggs found in stomachs intestines both moribund dead fish, respectively. Two strains A. isolated kidneys. Scanning electron microscope microphotographs stomach revealed presence numerous penetrating deep into gastric tissue, diffuse lesions filled bacilliform bacteria. Histopathological examination showed multifocal eosinophilic infiltrate, gland epithelial necrosis sloughed necrotic debris lumen. report coronatus, foratus, malbergi parasitizing Brazil. These findings indicate that synergic parasites (E. E. malbergi) may induce high mortalities

Language: Английский

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Pathogenic Aeromonas spp in Amazonian fish: Virulence genes and susceptibility in Piaractus brachypomus, the main native aquaculture species in Peru

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 33, P. 101811 - 101811

Published: Nov. 2, 2023

The aim of this study was to isolate and identify Aeromonas species, characterize their virulence genes factors, evaluate lethality in Piaractus brachypomus. Presumptive (n = 34) were selected from 44 bacteria retrieved diseased Amazonian fish. Nineteen isolates chosen based on fish group, the location where isolated, colony morphology. Isolates biochemically molecularly identified as hydrophila, A. dhakensis, veronii, jandaei, caviae using API 20E, 20NE analysis 16 S rRNA gyrB genes, respectively. Eight virulences (aer, altA, act, hly, ser, lip, fla ahyB) found one dhakensis two hydrophila isolates. another harbored four, three All +, ahyB + lip except that also presented ser altA. P. brachypomus (9 ± 1 cm), infected intraperitoneally (4 ×106, 4 ×107, ×108 CFU/fish), showed susceptibility all each harboring a different profile. Both caused > 80% mortality at lowest evaluated concentration (∼106 CFU/fish). veronii jandaei produced 20% 70% mortality, respectively, ∼107 CFU/fish, but with lower intensity compared dhakensis. At highest (∼108 while ranging 50% 80%. Remarkably, jandei resulted 100% level, similar This report confirms variety species infecting farmed native suggests may not always be an indicator virulence. Moreover, brachypomus, economically important aquaculture could susceptible only high mentioned other less observed such caviae, should taken into account health surveillance for implementation disease prevention strategies.

Language: Английский

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