Investigation of the effects of Periplaneta americana (L.) extract on ischemic stroke based on combined multi-omics of gut microbiota DOI Creative Commons
Xin Yang, Hong Chen,

Tianmin Guan

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 28, 2024

Ischemic stroke (IS) is a highly lethal type of cardiovascular and cerebrovascular disease. Improving survival rates promoting recovery in patients with IS pose significant challenges, however, recent research has identified the gut–brain axis as therapeutic target. In this study, we evaluated regulatory effect Periplaneta americana (L.) extract (PAS840), which established anti-inflammatory, antioxidant, neuroprotective effects, on gut microbiota using rat model temporary middle cerebral artery occlusion (tMCAO). We protective effects PAS840 brain damage rats through TTC (triphenyltetrazolium chloride), Nissl staining, pathological section analysis. Additionally, investigated impact metabolites 16S rRNA sequencing, untargeted metabolomics contents, transcriptomics analyses tissues to explore its mechanism action. intervention resulted changes microbiota, including an increase abundance probiotic flora, decrease harmful metabolite profiles. It also attenuated damage, decreased platelet activity, inhibited oxidative stress genes related inflammation, improved neurological function rats. These findings suggest that preventive against via by regulating metabolites. Accordingly, candidate drug for further research.

Language: Английский

Targeting gut microbiota and metabolism as the major probiotic mechanism - An evidence-based review DOI Creative Commons
Teng Ma, Xin Shen, Xuan Shi

et al.

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 138, P. 178 - 198

Published: June 11, 2023

The human gut hosts complex microbial communities, which are linked to health and disease. microbiota is continuously reshaped by multiple environmental factors, especially diet. Gut dysbiosis may promote various metabolic, neurological, intestinal, cardiovascular diseases, even tumor development. Probiotics have shown promising prophylactic, mitigating, or curative effects when use as nutrient supplements adjunctive therapy. These beneficial and/or symptom alleviation often accompanied microbiome metabolome changes. Differing from other published review articles, here we summarized the latest knowledge of evidence-based health-promoting probiotics scouring literature recent animal probiotic intervention studies. To elucidate mechanisms in enhancing intestinal homeostasis, barrier function, host immunity, through modulating its metabolites. Meanwhile, also discussed challenges data analysis clinical research comparison between laboratories, provides an outlook for future perspectives applications probiotics. achieved modulation. Current discussed. Finally, provided.

Language: Английский

Citations

147
Immune regulation of the gut-brain axis and lung-brain axis involved in ischemic stroke DOI Creative Commons

Xiaoqiang Xie,

Lei Wang, Shan‐Shan Dong

et al.

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(3), P. 519 - 528

Published: July 20, 2023

Abstract Local ischemia often causes a series of inflammatory reactions when both brain immune cells and the peripheral response are activated. In human body, gut lung regarded as key reactional targets that initiated by ischemic attacks. Mucosal microorganisms play an important role in regulation metabolism affect blood-brain barrier permeability. addition to relationship between organs central areas intestine also interact among each other. Here, we review molecular cellular mechanisms involved pathways inflammation across gut-brain axis lung-brain axis. We found abnormal intestinal flora, microenvironment, infection, chronic diseases, mechanical ventilation can worsen outcome stroke. This introduces influence on lungs after stroke, highlighting bidirectional feedback effect gut, lungs, brain.

Language: Английский

Citations

32
Multi-Omics Analysis of the Gut-Brain Axis Elucidates Therapeutic Mechanisms of Guhong Injection in the Treatment of Ischemic Stroke DOI Open Access

Pingting Mao,

Jianhua Hu,

Xi Mai

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1560 - 1560

Published: Feb. 12, 2025

Guhong injection (GH) is a compound preparation widely utilized in the treatment of cerebrovascular diseases. Accumulating evidence indicates that gut microbiota implicated development ischemic stroke (IS). However, although therapeutic potential GH IS may be mediated through microbiota, intricate relationships among gut-brain axis, biomarkers, and target proteins remain to completely explained. A rat model middle cerebral artery occlusion (MCAO) was investigate impact on IS. Our 16S rRNA sequence analysis revealed markedly enhanced α-diversity intestinal microbiome rectified imbalance short-chain fatty acids (SCFAs). Metabolomic indicated reversed 45 biomarkers 6 disordered metabolic pathways MCAO rats. Among these, arachidonic acid, α-linolenic fructose, mannose were closely associated with comprising Lactobacillus modulated by GH. Furthermore, IS-related signaling pathways, including inflammation, autophagy, oxidative stress, apoptosis, significantly three microbial species influenced The efficacy context multiple involving SCFAs, proteins. This process partly relies axis.

Language: Английский

Citations

1
“Sentinel or accomplice”: gut microbiota and microglia crosstalk in disorders of gut–brain interaction DOI Creative Commons
Haonan Zheng, Cunzheng Zhang, Jindong Zhang

et al.

Protein & Cell, Journal Year: 2023, Volume and Issue: 14(10), P. 726 - 742

Published: April 19, 2023

Abstract Abnormal brain–gut interaction is considered the core pathological mechanism behind disorders of gut–brain (DGBI), in which intestinal microbiota plays an important role. Microglia are “sentinels” central nervous system (CNS), participate tissue damage caused by traumatic brain injury, resist infection and neurogenesis, involved occurrence various neurological diseases. With in-depth research on DGBI, we could find between microglia that they jointly especially individuals with comorbidities mental disorders, such as irritable bowel syndrome (IBS). This bidirectional regulation provides a new direction for treatment DGBI. In this review, focus role underlying gut IBS, corresponding clinical application prospects highlight its potential to treat DGBI psychiatric comorbidities.

Language: Английский

Citations

14
Microglia in Microbiota-Gut-Brain Axis: A Hub in Epilepsy DOI
Yuyang Liu,

Ningkang Jia,

Chuqi Tang

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(9), P. 7109 - 7126

Published: Feb. 17, 2024

Language: Английский

Citations

5
Mechanisms of glutamate metabolic function and dysfunction in vascular dementia DOI Creative Commons
Jiawen Wang, Yingmei Zhang, Ning Tian

et al.

Neuroprotection/Neuroprotection (Chichester, England. Print), Journal Year: 2024, Volume and Issue: 2(1), P. 33 - 48

Published: Feb. 27, 2024

Abstract As the global population ages, research on pathogenesis and treatment options for older patients with dementia has become increasingly important. Vascular (VaD), second most frequent type of dementia, is characterized by vascular impairment caused inadequate blood supply to brain. VaD a complex neurological disorder involving multiple cells signaling pathways, its prevention pose clinical challenges significant behavioral implications. Glutamate, abundant amino acid in brain, plays critical role as an excitatory neurotransmitter, impacting cognitive function, learning, memory. Abnormal glutamate metabolism been closely linked reduced flow brain can lead excessive accumulation, resulting neuronal death. This article highlights connection between metabolism, aiming identify better methods preventing treating via regulating metabolism.

Language: Английский

Citations

4
Metabolic control of microglia in health and disease DOI
Gloria Colombo, Katia Monsorno, Rosa Chiara Paolicelli

et al.

Handbook of clinical neurology, Journal Year: 2025, Volume and Issue: unknown, P. 143 - 159

Published: Jan. 1, 2025

Language: Английский

Citations

0
Rhubarb with Different Cooking Methods Restored the Gut Microbiota Dysbiosis and SCFAs in Ischemic Stroke Mice DOI Creative Commons

Jing Xu,

Taotao Liu,

Fuzhu Pan

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Ischemic stroke is a significant public health problem worldwide. Growing evidence has shown gut microbiota served vital function in ischemic stroke. Rhubarb, always used after processing, promising therapy for However, the possible mechanism of rhubarb with different cooking methods remained unclear. Herein, constitutes steaming (SP), raw (RP), and nine sun-drying (NSP) were identified via LC-QTOF-MS. The middle cerebral artery occlusion (MCAO) mice model was constructed. Infarction area, neurological score, Nissl staining, IBA-1 immunofluorescence, ELISA performed to confirm neuroprotective effect SP, RP, NSP. fetal studied 16sRNA sequencing, level short-chain fatty acids (SCFAs) brain measured GC-MS. signature through PICRUSt2; studied. NSP alleviated dysfunction, decreased inflammation, suppressed dysbiosis microbiota, restored SCFA-producing bacteria, enhanced SCFA MCAO mice. Moreover, SP enriched proportion anti-inflammation beneficial deleted pro-inflammation. It observed energy metabolism involved rhubarb; activities COXI Na+-K+-ATPase increased treatment Furthermore, expression GLUT4 PFK1 (the metabolism-related genes) elevated administration. In this study, it provided proof rhubarb. Rhubarb regulated alleviate

Language: Английский

Citations

0
The Association Between Branched-Chain Amino Acid Concentrations and the Risk of Autism Spectrum Disorder in Preschool-Aged Children DOI Creative Commons
Qi Gao, Dan Bi, Bingbing Li

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(8), P. 6031 - 6044

Published: Jan. 24, 2024

Several studies have linked branched-chain amino acid (BCAA) metabolism disorders with autism spectrum disorder (ASD), but the results been inconsistent. The purpose of this study was to explore association between BCAA concentrations and risk ASD. A total 313 participants were recruited from two tertiary referral hospitals May 2018 July 2021. Concentrations BCAAs in dried blood spots analyzed using liquid chromatography-tandem mass spectrometry-based analysis. Multivariate analyses restricted cubic spline models used identify ASD, a nomogram developed by multivariate logistic regression determined receiver operating characteristic curve analysis calibration BCAA, valine, leucine/isoleucine higher ASD group, all them positively non-linearly associated even after adjusting for potential confounding factors such as age, gender, body index, (P < 0.05). integrating valine showed good discriminant AUC value 0.756 (95% CI 0.676-0.835). model could yield net benefits across reasonable range thresholds. In stratified analysis, diagnostic ability more pronounced children older than 3 years. We provide evidence that increased levels are presented here can serve marker early diagnosis

Language: Английский

Citations

3
Dietary Supplementation with Nervonic Acid Ameliorates Cerebral Ischemia‐Reperfusion Injury by Modulating of Gut Microbiota Composition‐Fecal Metabolites Interaction DOI
Hui Zhou, Ziyi Chen, Jingbin Li

et al.

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: 68(8)

Published: April 1, 2024

Scope Cerebral ischemia‐reperfusion (IR) injury stands as a prominent global contributor to disability and mortality. Nervonic acid (NA), bioactive elongated monounsaturated fatty acid, holds pivotal significance in human physiological well‐being. This research aims explore the prophylactic effects fundamental mechanisms of NA rat model cerebral IR injury. Methods results Through induction middle artery occlusion, this study establishes comprehensively assesses pharmacodynamic impacts pretreatment. evaluation involves behavioral analyses, histopathological examinations, quantification serum markers. Detailed nervonic acid's are revealed through fecal metabolomics 16S rRNA sequencing analyses. Our findings robustly support capacity ameliorate neurological impairments rats afflicted with Beyond its benefits, demonstrates potential by rectifying metabolic perturbations across diverse pathways, particularly those pertinent unsaturated metabolism. Additionally, emerges modulator gut microbiota composition, notably selectively enhancing vital genera like Lactobacillus . Conclusion These comprehensive highlight incorporating functional component dietary interventions aimed at targeting

Language: Английский

Citations

3