Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 127, P. 106770 - 106770
Published: March 29, 2025
Language: Английский
International Immunopharmacology, Journal Year: 2025, Volume and Issue: 150, P. 114321 - 114321
Published: Feb. 18, 2025
Language: Английский
Citations
0
Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)
Published: Feb. 19, 2025
This study aims to investigate the therapeutic effects of modified Huangqi Chifeng decoction (MHCD) on proteinuria in membranous nephropathy (MN) and its potential protective podocytes. Furthermore, we explored whether these are associated with inhibition nuclear factor kappa-B (NF-κB) pathway. Passive Heymann nephritis (PHN) rat model was applied a single tail vein injection sheep anti-rat Fx1A serum (0.4 ml/100g). All rats were divided into four groups: normal group, PHN benazepril group (10 mg/kg), MHCD (12.5 g/kg), treated for 6 weeks. 24-hour urine protein levels biochemical parameters measured. Optical microscopy transmission electron performed assess pathological changes renal tissues. Additionally, expression IgG, C5b-9, nephrin, podocin, Wilms' tumor gene 1 (WT-1), NF-κB p65 evaluated. exhibited progressive over time. However, treatment significantly reduced triglyceride, while increased albumin. Moreover, alleviated damage tissues, IgG membrane attack complex (C5b-9). Immunohistochemistry analysis revealed that WT-1. Western blot showed nephrin podocin inhibiting activation p65. Our findings indicate exert reno-protective experimental MN by alleviating podocyte
Language: Английский
Citations
0
Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)
Published: March 10, 2025
Membranous nephropathy (MN) is a primary glomerular disease commonly causing adult nephrotic syndrome. Characterized by thickened capillary walls due to immune complex deposition, MN autoimmune disorder. Its pathogenesis involves deposit formation, complement activation, and heightened risk of renal failure. Central system dysfunction, particularly the dysregulation B T cell responses. cells contribute injury through production autoantibodies, IgG targeting phospholipase A2 receptor (PLA2R) on podocytes, while modulate responses that influence progression. Metabolic reprogramming alters lymphocyte survival, differentiation, proliferation, function, potentially triggering processes. Although link between metabolism remains underexplored, this review highlights recent advances in understanding its role MN. These insights may provide novel biomarkers therapeutic strategies for treatment. Immune membranous drives Dysregulated B/T metabolic trigger autoimmunity, offering potential therapies
Language: Английский
Citations
0
Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown
Published: March 12, 2025
Language: Английский
Citations
0
iMeta, Journal Year: 2025, Volume and Issue: 4(2)
Published: Feb. 26, 2025
Abstract Clinical nutritional support is recognized by Klinefner's Surgery as one of the four pivotal advancements in surgical practice during 20th century. Surgeons regard clinical nutrition a “life‐saving” discipline, preserving lives numerous critically ill patients and facilitating success many procedures. Parenteral (PN) serves crucial component therapy, while range complications associated with total parenteral (TPN) can significantly undermine efficacy patient treatment. Impaired intestinal homeostasis strongly occurrence progression TPN‐related infections, yet underlying mechanisms remain poorly understood. In this study, RNA sequencing single‐cell (scRNA‐Seq) revealed that reduced secretion interleukin‐22 (IL‐22) Group 3 innate lymphoid cells (ILC3s) significant factor contributing to onset infections. Additionally, through 16S ribosomal (16S rRNA) gene gut microbiota from chronic failure metagenomic analysis mice, we observed TPN abundance Lactobacillus murinus ( L. ), supplementation could promote IL‐22 ILC3s. Mechanistically, upregulates indole‐3‐carboxylic acid, which activates nuclear receptor Rorγt stimulate This pathway strengthens barrier integrity reduces infection susceptibility. Our findings enhance our understanding driving highlighting critical role maintaining immune improving outcomes.
Language: Английский
Citations
0
Renal Failure, Journal Year: 2025, Volume and Issue: 47(1)
Published: March 13, 2025
Membranous nephropathy (MN), a leading cause of adult nephrotic syndrome and renal failure, has been linked to gut microbiota (GM) their metabolites. However, direct causal relationships therapeutic implications remain unclear. We utilized comprehensive GWAS dataset that encompasses GM, metabolites, MN through two-sample Mendelian randomization (MR) analyses, bidirectional MR evaluations, detailed sensitivity tests. identified strong associations between nine specific types including class Clostridia (OR = 1.816, 95%CI: 1.021-3.236, p .042), Melainabacteria 0.661, 0.439-0.996, .048), order Gastranaerophilales 0.689, 0.480-0.996, .044), genus Alistipes 0.480, 0.223-0.998, .049), Butyricicoccus 0.464, 0.216-0.995, Butyrivibrio 0.799, 0.639-0.998, Ruminococcaceae UCG003 0.563, 0.362-0.877, .011), Streptococcus 0.619, 0.393-0.973, .038), Oscillibacter 1.90, 1.06-3.40, .031). Additionally, the metabolite tryptophan also exhibited significant influence on 0.852, 0.754-0.963, .010). Sensitivity reverse analyses confirmed robustness these findings. Further exploration using gutMGene database suggests GM may by affecting release inflammatory factors modulating pathways. This study offers understanding links MN, which highlight potential pathways for developing new preventive strategies this condition.
Language: Английский
Citations
0
Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 557 - 557
Published: April 10, 2025
Fecal calprotectin (FC) is a biomarker of intestinal inflammation widely used in the assessment gastrointestinal disorders. However, its role chronic kidney disease (CKD) remains unclear. Given growing recognition gut-kidney axis CKD pathophysiology, this study aimed to investigate association between FC levels, systemic inflammation, renal outcomes, and mortality patients. We enrolled total 515 patients who underwent fecal measurement 2016 2023. After applying exclusion criteria (inflammatory bowel disease, ongoing replacement therapy, or incomplete laboratory data), 260 were included final analysis stratified into low-FC (<102 μg/g, n = 130) high-FC (≥102 groups based on median value. Factors associated with progression patient survival analyzed. Patients group μg/g) significantly older (72.8 ± 14.63 vs. 64.02 18.15 years, p < 0.0001) had higher prevalence diabetes mellitus (55.38% 42.31%, 0.0349), heart failure (21.54% 7.69%, 0.0016), history acute injury (33.85% 18.46%, 0.0048). Elevated was independently increased risk (hazards ratio [HR] 1.658, 95% confidence interval [CI] 1.034-2.658, 0.0357) rates (48.36 18.46 per 100,000 person-years). Subgroup analyses revealed stronger associations males (HR 2.160, CI 1.046-4.463, 0.0375), elderly (≥75 years) 2.122, 1.209-3.725, 0.0088), non-diabetic 2.487, 1.141-5.421, 0.0219). While not end-stage (ESKD) (odds [OR] 1.289, 0.455-3.650, 0.6323), levels paradoxically predicted slower estimated glomerular filtration rate (eGFR) decline (OR 2.763, 1.139-6.699, 0.0245). Combined both elevated high-sensitivity C-reactive protein (hs-CRP) highest 3.504, 1.163-10.554, compared those low markers. potential prognostic for patients, traditional inflammatory Further research warranted elucidate mechanisms underlying paradoxical relationship outcomes stratification therapeutic targeting CKD.
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12
Published: April 30, 2025
Worldwide, kidney disease has grown to be an important global public health agenda that reduces longevity. Medical institutions around the globe should enhance screening efforts for disease, facilitate early detection, diagnosis, and intervention. Common methods nephropathy encompass renal tissue biopsy, urine dry chemistry tests, formed element analysis, urine-specific protein assays, among others. These methodologies evaluate by scrutinizing a spectrum of biomarkers. Precise classification quantitative analysis these biomarkers can assist in determining site extent injury, as well assessing treatment efficacy prognosis. In this paper, we reviewed also integration multiple With aim reasonable applying markers accurate scientific management thereby mitigating threat posed human health.
Language: Английский
Citations
0
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16
Published: April 30, 2025
Background Diabetic nephropathy (DN) is a serious complication of diabetes and one the leading causes end-stage renal disease. Huangkui capsule (HKC), traditional Chinese patent medicine, widely used in clinical practice for treatment chronic glomerulonephritis. However, therapeutic effects underlying mechanisms HKC DN remain poorly understood. Methods was induced db/db mice, which were randomly divided into DN, HKC-L, HKC-H IRB groups, db/m mice served as Control group. Biochemical indices blood urine samples from measured, HE staining, Masson staining PAS to verify anti-DN effect HKC. The levels ROS expression Nrf 2 pathway-related proteins mRNAs detected. Metabonomic analysis investigate role tryptophan metabolism regulation by HK-2 cells establish model high-glucose (HG) injury vitro , given supplementary verification. Sarpogrelate hydrochloride (SH) combined with HKC, 5-HT 2A R inhibitor, pathway an model. Results Treatment significantly inhibited increase glucose Urinary albumin/creatinine ratio (UACR), improved kidney signs reduced level oxidative stress through Keap1/ /HO-1 pathway. revealed that involved process improves can regulate improve regulation. also injuries HG cell . SH administration inhibiting could inhibit synthesis HG. Conclusion Our study demonstrate regulating Keap1/Nrf pathway, provides new insight use DN.
Language: Английский
Citations
0
Renal Failure, Journal Year: 2024, Volume and Issue: 46(1)
Published: May 21, 2024
Research has showcased a correlation between disruptions in gut microbiota and primary membranous nephropathy (pMN), giving rise to the concept of 'gut-kidney axis'. However, precise relationship pMN remains elusive. Hence, this study endeavors investigate whether causal exists utilizing Mendelian randomization (MR) analysis.
Language: Английский
Citations
3