Published: Jan. 1, 2025
Language: Английский
Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103621 - 103621
Published: March 1, 2025
Kelch ECH-associating protein 1 (Keap1)-Nuclear factor erythroid 2-related 2 (Nrf2) axis is crucial for regulating oxidative stress and inflammatory responses in acute pneumonia. Sphaeropsidin A (SA) a antioxidant diterpenoid isolated from Sphaeropsis sapinea f. sp. cupressi, discovered as novel Nrf2 agonist by our research group previously. However, the accurate function mechanism of SA treating pneumonia are still unknown. The therapeutic effect was evaluated LPS-induced mice. underlying action then analyzed transcriptomics. direct target identified through synthesis SA-biotin probe, binding amino acid residues were found verified LC-MS/MS analysis site-specific mutation. Finally, knockout mice employed to verify SA. Our data indicated that significantly inhibited via up-regulating Nrf2, inhibiting NLRP3 inflammasome NF-κB activation, Keap1 Specifically, effective dose only mg/kg. selectively covalent bound cysteine 151 residue (Cys151). mediated activation reduced level ROS, thereby inflammasome. Besides, formed hydrogen bond with ASP48 ASC, blocking its oligomerization This study indicates might be new molecule activate promising drug candidate or lead therapy Nrf2/NF-κB/NLRP3 axis.
Language: Английский
Citations
0
Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 314 - 314
Published: March 30, 2025
There is currently no research on the application evaluation of R. mucilaginosa yeast culture (RYC) in animal production. Therefore, this study investigated effects RYC antioxidant capacity, lipid metabolism, and immunity sheep livers. Twenty-four 3-month-old Duhan male (36 ± 4 kg) were divided into four groups. The control group received a basal diet, L diet + 10 g/sheep/day RYC, M 20 H 40 RYC. trial lasted for 75 days. results showed that content glutathione peroxidase livers was significantly increased by 26.6%, malondialdehyde decreased 38% (p < 0.05). Additionally, serum levels total cholesterol, triglycerides, high-density lipoprotein low-density cholesterol decreased; liver C16:0 C18:2n6C C20:1 Furthermore, contents cytokines TNF-α IFN-γ from also 20% 24.8%, respectively These findings suggest supplementation with can enhance improve reduce inflammation livers, which advantageous farming healthy sheep.
Language: Английский
Citations
0
Neuroreport, Journal Year: 2025, Volume and Issue: 36(6), P. 327 - 335
Published: March 26, 2025
Acute cerebral infarction (CI) is characterized by acute onset, high disability rate, and morbidity which seriously threatens the health safety of people places a heavy burden on individuals country. This study aimed to explore effects butylphthalide nerve cell ferroptosis in CI rats its underlying mechanisms. Middle artery occlusion (MCAO) rat model was used effect vivo oxygen glucose deprivation (OGD) vitro . Our findings demonstrated that markedly reduced oxidative damage brains MCAO rats. Furthermore, we found upregulated netrin-1/deleted colorectal cancer (DCC)/vascular endothelial growth factor (VEGF) signaling axis, regulates NF-E2-related factor-2 (NRF2) expression contributes OGD-treated HT22 cells. Collectively, our indicate inhibits stress-mediated cells modulating netrin-1/DCC/VEGF/NRF2 axis. In conclusion, results reveal novel mechanism for protection CIs butylphthalide.
Language: Английский
Citations
0
Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217697 - 217697
Published: April 1, 2025
Language: Английский
Citations
0
Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 105014 - 105014
Published: April 1, 2025
Language: Английский
Citations
0
Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 128, P. 106842 - 106842
Published: April 25, 2025
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: May 1, 2025
Diabetes and chronic kidney disease (CKD) increase susceptibility to bacterial infections, particularly Staphylococcus aureus, which is associated with highmortality in CKD patients. Dysregulated macrophage activity excessive oxidative stress exacerbate immune dysfunction inflammation these conditions. Nrf2 (nuclear factor erythroid 2-related 2) a key regulator of antioxidant defenses function. CDDO-Me, synthetic triterpenoid, activates Nrf2, providing anti-inflammatoryeffects. However, its precise role modulating activity, polarization, clearance remains unclear. The effects CDDO-Me on function were evaluated vitro (THP-1 RAW 264.7 macrophages) an vivo knockout mouse model. activation was assessed via Western blot luciferase reporter assays, measured using CellROX reagent, inflammatory responses quantified by RT-qPCR. Intracellular S. aureus survival polarization markers analyzed investigate the enhancing bactericidal activity. Our results showed that activated signaling pathway, reducing macrophages downregulating pro-inflammatory cytokines (IL-1β, TNF-α). It modulated decreasing M1 M2 marker expression, significantly enhanced against aureus. These Nrf2-dependent, as demonstrated models. ability regulate stress, inflammation, underscores therapeutic potential for managing infectious diseases indiabetes CKD.
Language: Английский
Citations
0
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 119985 - 119985
Published: May 1, 2025
Language: Английский
Citations
0
The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown
Published: May 20, 2025
Abstract Oxygen and sulfur, both members of the chalcogen group (group 16 elements), play fundamental roles in life. Ancient organisms primarily utilized sulfur for energy metabolism, while rise atmospheric oxygen facilitated evolution aerobic organisms, enabling highly efficient production. Nevertheless, all modern aerobes anaerobes, must protect themselves from toxicity. Interestingly, still rely on survival. This dependence has been illuminated by recent discovery supersulfides, a novel class biomolecules, made possible through advancements technology analytical methods. These breakthroughs are reshaping our understanding biological processes emphasizing intricate interplay between regulating essential redox reactions. review summarizes latest insights into oxygen, their interdependence key processes, contributions to adaptive responses environmental stressors. By exploring these interactions, we aim provide comprehensive perspective how elements drive survival strategies across diverse life forms, highlighting indispensable human health sustenance
Language: Английский
Citations
0
Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 198 - 198
Published: Jan. 11, 2024
Epilobium hirsutum L., commonly known as hairy willowherb, is a perennial herbaceous plant native to Europe and Asia. In Romania, the genus includes 17 species that are used in folk medicine for various purposes. This study aimed investigate anti-inflammatory antitumor potential of optimized extract (EH) animal models. The first investigated properties EH model was carrageenan-induced paw inflammation. Wistar rats were divided into three groups: negative control, positive control treated with indomethacin, group extract. Oxidative stress markers, cytokine levels, protein expressions assessed. demonstrated comparable those group. second study, effects assessed using tumor Ehrlich ascites carcinoma. Swiss albino mice four negative, cyclophosphamide (Cph), Group 3 Cph extract, 4 alone. Samples from fluid, liver, heart analyzed evaluate oxidative stress, inflammation, cancer markers. showed reduction tumor-associated inflammation stress. Overall, exhibited promising models studied. These findings suggest its natural adjuvant therapeutic agent addressing induced by different pathologies.
Language: Английский
Citations
3