Piezo1 Ion Channels Regulate the Formation and Spreading of Human Endometrial Mesenchymal Stem Cell Spheroids DOI Open Access
Zuleikha M. Khairullina, Valeria Y. Vasileva, V. I. Chubinskiy-Nadezhdin

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2474 - 2474

Published: March 10, 2025

Mesenchymal stem cells obtained from desquamated endometrium (eMSCs) are considered as reliable and promising objects for cell-based therapy. eMSCs aggregated into three-dimensional (3D) spheroids demonstrate greater efficiency compared to monolayer 2D eMSCs. However, molecular processes specific mechanisms regulating the effectiveness of remain unknown. Regulation a number physiological reactions in MSCs is associated with functioning Ca2+-permeable mechanosensitive Piezo1 channels. In our previous study, we showed that selective activation by its agonist Yoda1 controls migratory activity Here, aimed determine effect on eMSC spheroid formation spreading. PIEZO1 mRNA expression was lower culture. Spheroids formed or spread presence demonstrated spreading rates control (Yoda1-free) spheroids. The depended substrate stiffness, whereas had similar regardless surface properties. Our results several Piezo1-dependent could be modulated activation.

Language: Английский

Involvement of Piezo 1 in inhibition of shear‐induced platelet activation and arterial thrombosis by ginsenoside Rb1 DOI Creative Commons

Yilin Wang,

Lu Liu,

Lee Jia

et al.

British Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Shear-induced platelet activation and aggregation (SIPA) play crucial roles in arterial thrombosis. Piezo1 is a mechanosensitive calcium channel that promotes hyperactivation under pathological high-shear conditions. This study explores the function of SIPA thrombosis, inhibitory effects mechanisms ginsenoside Rb1 on these processes. Transgenic mice with platelet-specific deficiency (Piezo1ΔPlt) were used to elucidate role A microfluidic system was employed assess aggregation, influx, calpain activity, talin cleavage, integrin αIIbβ3 P-selectin expression shear flow. Cellular thermal shift assay determine binding between Piezo1. Folts-like model evaluate antithrombotic Rb1. platelets reduced induced by high rate 4000 s-1 attenuated thrombosis mouse model. inhibited an IC50 10.8 μM. shear-induced Ca2+-dependent as well thrombus formation Piezo1fl/fl mice. significantly improved stability Treatment Piezo1ΔPlt did not exhibit further Platelet essential for shear. exerted anti-platelet anti-thrombotic at rates via channels, providing potential candidate antiplatelet therapeutic agent.

Language: Английский

Citations

0
Effect of Mechanically Activated Calcium Channels on Passive Stiffness and Contraction Amplitude of Slow Muscle DOI

K. V. Sergeeva,

С. А. Тыганов,

К. А. Зарипова

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 61(1), P. 115 - 121

Published: Jan. 1, 2025

Language: Английский

Citations

0
Piezo1 Ion Channels Regulate the Formation and Spreading of Human Endometrial Mesenchymal Stem Cell Spheroids DOI Open Access
Zuleikha M. Khairullina, Valeria Y. Vasileva, V. I. Chubinskiy-Nadezhdin

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2474 - 2474

Published: March 10, 2025

Mesenchymal stem cells obtained from desquamated endometrium (eMSCs) are considered as reliable and promising objects for cell-based therapy. eMSCs aggregated into three-dimensional (3D) spheroids demonstrate greater efficiency compared to monolayer 2D eMSCs. However, molecular processes specific mechanisms regulating the effectiveness of remain unknown. Regulation a number physiological reactions in MSCs is associated with functioning Ca2+-permeable mechanosensitive Piezo1 channels. In our previous study, we showed that selective activation by its agonist Yoda1 controls migratory activity Here, aimed determine effect on eMSC spheroid formation spreading. PIEZO1 mRNA expression was lower culture. Spheroids formed or spread presence demonstrated spreading rates control (Yoda1-free) spheroids. The depended substrate stiffness, whereas had similar regardless surface properties. Our results several Piezo1-dependent could be modulated activation.

Language: Английский

Citations

0