Theriogenology, Journal Year: 2024, Volume and Issue: 232, P. 79 - 86
Published: Nov. 2, 2024
Language: Английский
Environmental Research, Journal Year: 2024, Volume and Issue: 252, P. 118799 - 118799
Published: March 27, 2024
Language: Английский
Citations
8
Annual Review of Animal Biosciences, Journal Year: 2023, Volume and Issue: 12(1), P. 91 - 112
Published: Nov. 21, 2023
Cloning as it relates to the animal kingdom generally refers production of genetically identical individuals. Because cloning is increasingly subject renewed attention a tool for rescuing endangered or extinct species, seems timely dissect role numerous reproductive techniques encompassed by this term in species conservation. Although typically associated with somatic cell nuclear transfer, recent advent additional that allow genome replication without genetic recombination demands use induced pluripotent stem cells generate gametes embryos, well older methods such embryo splitting, all be included discussion. Additionally, phenomenon natural (e.g., subset fish, birds, invertebrates, and reptilian reproduce via parthenogenesis) must also pointed out. Beyond biology these are practical considerations ethics using procedures species. All examined concert determine whether has place Therefore, we synthesize progress ethical aspects they pertain
Language: Английский
Citations
16
Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 148 - 148
Published: Jan. 9, 2025
A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and selected. The objective this study was investigate the morphokinetic parameters fishing cat-domestic cat interspecies somatic cell nuclear transfer (iSCNT) from one-cell stages, particular, cleavage patterns first division iSCNT IVF embryos, as these play central role euploidy. Domestic vitro fertilization (IVF) were set up controls. results show that morula development rates significantly lower compared their counterparts. All earlier time points embryonic before onset blastulation delayed when with In iSCNT, normal (defined those developed stage) took longer reach stage, morulas more likely undergo compaction, arrested Direct is morphological aberration, seen greater prevalence than control embryos; aberrant displayed rate had undergone cleavage. conclusion, at early stages could used predict potential for stage. imaging potentially tool selecting transfer, hence, improving feline efficiency.
Language: Английский
Citations
0
Zoo Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Human interference reduces wild felid populations. Somatic cell nuclear transfer and the use of induced pluripotent stem cells are potential conservation strategies. To improve efficiency these strategies, it is essential to establish adequate protocols for synchronization in G0/G1 phase cycle. Cell cycle can arrest progression by inhibiting factors involved duplication. However, this step varies among felids has not been successful some species. In addition, effect on applications remains unclear. Therefore, review highlights primary differences that cause variability, most promising results, methods used. Finally, importance biotechnologies involving reprogramming somatic highlighted.
Language: Английский
Citations
0
Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 989 - 989
Published: March 29, 2025
Over 40 years ago, scientists imagined ways cloning could aid conservation of threatened taxa. The Dolly the sheep from adult somatic cells in 1996 was breakthrough that finally enabled potential technology. Until 2020s, research efforts yielded no management applications, leading many to believe is not yet an effective tool. In strong contrast, domestic taxa are cloned routinely for scientific and commercial purposes. this review, we sought understand reasons these divergent trends. We scoured peer-reviewed gray literature sent direct inquiries analyze a more comprehensive history field than analyzed previous reviews. While most reviewers concluded lack reproductive knowledge wildlife species has hindered advances wider found resource limitations (e.g., numbers surrogates, sustainable funding) widely held misconceptions about significant contributors stagnation field. Recent successes programs endangered black-footed ferret (Mustela nigripes) Przewalski's horse (Equus przewalskii), world's first true applied-conservation efforts, demonstrating can be used impact present. When viewed alongside long achievements, emphasize value investing science resources needed meaningfully integrate into management, especially with limited genetic diversity rely on maintenance small populations generations while conservationists work restore habitat mitigate threats wild.
Language: Английский
Citations
0
BioScience, Journal Year: 2024, Volume and Issue: 74(8), P. 561 - 566
Published: July 31, 2024
Abstract Earth's biodiversity is increasingly threatened and at risk. We propose a passive lunar biorepository for long-term storage of prioritized taxa live cryopreserved samples to safeguard support future space exploration planet terraforming. Our initial focus will be on cryopreserving animal skin with fibroblast cells. An exemplar system has been developed using fish fins from the Starry Goby, Asterropteryx semipunctata. Samples expanded into cells, recryopreserved, then tested in an Earth-based laboratory robust packaging sensitivity radiation. Two key factors this are needs reduce damage radiation maintain near –196° Celsius. Certain sites poles may meet these criteria. If possible, further testing would occur International Space Station prior Moon. To secure positive shared future, open call participate decades-long program.
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13675 - 13675
Published: Dec. 21, 2024
Taking into consideration recent reports on the successful creation of cloned rhesus monkeys [...]
Language: Английский
Citations
2
Theriogenology Wild, Journal Year: 2024, Volume and Issue: 5, P. 100096 - 100096
Published: Jan. 1, 2024
Female rhinoceroses have an ovarian reserve that consists of immature oocytes in primordial follicles. Establishing methods to grow and culture these from those follicles the laboratory might fuel efforts towards vitro embryo production this species without need for repeated vivo oocyte collection. In depth understanding biology behind improvement assisted reproductive techniques (ARTs) is only option preventing disappearance functionally extinct such as northern white rhinoceros. follicle development can be obtained by knowledge on rhinoceros histology. addition known features, we identified numerous undescribed structural molecular characteristics adult southern (n=3). Ovarian sections were stained with H&E, PAS or Masson Trichrome analyses (hyaluronic acid detection, immunohistochemistry TUNEL assay) performed identify proliferation, extracellular matrix, pluripotency markers, hormones, enzymes, markers inflammation endocrine glands, blood vessel presence, apoptosis. Besides degenerating, deformed, luteinized follicles, analysis revealed several 'follicle-like structures' (FLS) deviated expected appearance. Most importantly, majority FLS did not contain any comprised a collection cells organised around fluid islands antral cavity. The discovery description aged may play role poor recovery rates ovum pick-up (OPU) older rhinoceroses. It remains investigated whether present ovaries other they serve general indicator success females.
Language: Английский
Citations
1
Tissue and Cell, Journal Year: 2024, Volume and Issue: 93, P. 102708 - 102708
Published: Dec. 25, 2024
Language: Английский
Citations
0
Animal Reproduction, Journal Year: 2024, Volume and Issue: 21(3)
Published: Jan. 1, 2024
Over the past 40 years, assisted reproductive technologies (ARTs) have grown significantly in scale and innovation, from bovine embryo industry's shift vivo derived to vitro produced embryos development of somatic cell-based approaches for production. Domestic animal models been instrumental ARTs wildlife species support One Plan Approach conservation that integrates situ ex population management strategies. While are not sole solution biodiversity crisis, they can offer opportunities maintain, even improve, genetic composition captive wild gene pools over time. This review focuses on application sperm (artificial insemination multiple ovulation/in transfer, respectively) species, highlighting impactful cases which significant progress or innovation has transpired. key messages following decades efforts this field is importance collaboration between researchers practitioners zoological, academic, governmental, private sectors.
Language: Английский
Citations
0